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Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells

Standard

Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells. / Zerangue, N; Malan, M J; Fried, S R; Dazin, P F; Jan, Y N; Jan, L Y; Schwappach, B.

in: P NATL ACAD SCI USA, Jahrgang 98, Nr. 5, 27.02.2001, S. 2431-6.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Zerangue, N, Malan, MJ, Fried, SR, Dazin, PF, Jan, YN, Jan, LY & Schwappach, B 2001, 'Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells', P NATL ACAD SCI USA, Jg. 98, Nr. 5, S. 2431-6. https://doi.org/10.1073/pnas.051630198

APA

Zerangue, N., Malan, M. J., Fried, S. R., Dazin, P. F., Jan, Y. N., Jan, L. Y., & Schwappach, B. (2001). Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells. P NATL ACAD SCI USA, 98(5), 2431-6. https://doi.org/10.1073/pnas.051630198

Vancouver

Zerangue N, Malan MJ, Fried SR, Dazin PF, Jan YN, Jan LY et al. Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells. P NATL ACAD SCI USA. 2001 Feb 27;98(5):2431-6. https://doi.org/10.1073/pnas.051630198

Bibtex

@article{f8f386f063f5426c84e708153fc9b25e,
title = "Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells",
abstract = "To improve the accuracy of predicting membrane protein sorting signals, we developed a general methodology for defining trafficking signal consensus sequences in the environment of the living cell. Our approach uses retroviral gene transfer to create combinatorial expression libraries of trafficking signal variants in mammalian cells, flow cytometry to sort cells based on trafficking phenotype, and quantitative trafficking assays to measure the efficacy of individual signals. Using this strategy to analyze arginine- and lysine-based endoplasmic reticulum localization signals, we demonstrate that small changes in the local sequence context dramatically alter signal strength, generating a broad spectrum of trafficking phenotypes. Finally, using sequences from our screen, we found that the potency of di-lysine, but not di-arginine, mediated endoplasmic reticulum localization was correlated with the strength of interaction with alpha-COP.",
keywords = "Amino Acid Sequence, Animals, Combinatorial Chemistry Techniques, Endoplasmic Reticulum/metabolism, Flow Cytometry, Fluorescent Antibody Technique, Genes, Reporter, Golgi Apparatus/metabolism, Molecular Sequence Data, Signal Transduction, Two-Hybrid System Techniques",
author = "N Zerangue and Malan, {M J} and Fried, {S R} and Dazin, {P F} and Jan, {Y N} and Jan, {L Y} and B Schwappach",
year = "2001",
month = feb,
day = "27",
doi = "10.1073/pnas.051630198",
language = "English",
volume = "98",
pages = "2431--6",
journal = "P NATL ACAD SCI USA",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "5",

}

RIS

TY - JOUR

T1 - Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells

AU - Zerangue, N

AU - Malan, M J

AU - Fried, S R

AU - Dazin, P F

AU - Jan, Y N

AU - Jan, L Y

AU - Schwappach, B

PY - 2001/2/27

Y1 - 2001/2/27

N2 - To improve the accuracy of predicting membrane protein sorting signals, we developed a general methodology for defining trafficking signal consensus sequences in the environment of the living cell. Our approach uses retroviral gene transfer to create combinatorial expression libraries of trafficking signal variants in mammalian cells, flow cytometry to sort cells based on trafficking phenotype, and quantitative trafficking assays to measure the efficacy of individual signals. Using this strategy to analyze arginine- and lysine-based endoplasmic reticulum localization signals, we demonstrate that small changes in the local sequence context dramatically alter signal strength, generating a broad spectrum of trafficking phenotypes. Finally, using sequences from our screen, we found that the potency of di-lysine, but not di-arginine, mediated endoplasmic reticulum localization was correlated with the strength of interaction with alpha-COP.

AB - To improve the accuracy of predicting membrane protein sorting signals, we developed a general methodology for defining trafficking signal consensus sequences in the environment of the living cell. Our approach uses retroviral gene transfer to create combinatorial expression libraries of trafficking signal variants in mammalian cells, flow cytometry to sort cells based on trafficking phenotype, and quantitative trafficking assays to measure the efficacy of individual signals. Using this strategy to analyze arginine- and lysine-based endoplasmic reticulum localization signals, we demonstrate that small changes in the local sequence context dramatically alter signal strength, generating a broad spectrum of trafficking phenotypes. Finally, using sequences from our screen, we found that the potency of di-lysine, but not di-arginine, mediated endoplasmic reticulum localization was correlated with the strength of interaction with alpha-COP.

KW - Amino Acid Sequence

KW - Animals

KW - Combinatorial Chemistry Techniques

KW - Endoplasmic Reticulum/metabolism

KW - Flow Cytometry

KW - Fluorescent Antibody Technique

KW - Genes, Reporter

KW - Golgi Apparatus/metabolism

KW - Molecular Sequence Data

KW - Signal Transduction

KW - Two-Hybrid System Techniques

U2 - 10.1073/pnas.051630198

DO - 10.1073/pnas.051630198

M3 - SCORING: Journal article

C2 - 11226256

VL - 98

SP - 2431

EP - 2436

JO - P NATL ACAD SCI USA

JF - P NATL ACAD SCI USA

SN - 0027-8424

IS - 5

ER -