An optimized fixation and extraction technique for high resolution of inositol phosphate signals in rodent brain.
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An optimized fixation and extraction technique for high resolution of inositol phosphate signals in rodent brain. / Lorke, Dietrich E; Gustke, Heike; Mayr, Georg W.
in: NEUROCHEM RES, Jahrgang 29, Nr. 10, 10, 2004, S. 1887-1896.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - An optimized fixation and extraction technique for high resolution of inositol phosphate signals in rodent brain.
AU - Lorke, Dietrich E
AU - Gustke, Heike
AU - Mayr, Georg W.
PY - 2004
Y1 - 2004
N2 - Members of lower and higher inositol phosphates distinctly participate in signal transduction (1). Relatively little is known regarding possible biological functions of inositol phosphates in functionally different areas of the intact brain. A detailed study on the regional distribution of biologically important inositol phosphates may help elucidate their physiological functions in different brain regions in the regional tissue context. We now show a novel technique which allows fixation and subsequent dissection of whole rat brains into small volume elements for mapping of the whole range of inositol phosphates from Ins(1,4,5)P3 to InsP6. The method has been successfully applied to investigate regional differences of a broader spectrum of inositol phosphates in microdissected brain tissue and to construct 3D-maps of these signaling compounds. The technique can be particularly well employed to investigate regional changes in the spectrum of higher inositol phosphates and phosphoinositides upon neuronal stimulation induced by motor activity or drug treatment.
AB - Members of lower and higher inositol phosphates distinctly participate in signal transduction (1). Relatively little is known regarding possible biological functions of inositol phosphates in functionally different areas of the intact brain. A detailed study on the regional distribution of biologically important inositol phosphates may help elucidate their physiological functions in different brain regions in the regional tissue context. We now show a novel technique which allows fixation and subsequent dissection of whole rat brains into small volume elements for mapping of the whole range of inositol phosphates from Ins(1,4,5)P3 to InsP6. The method has been successfully applied to investigate regional differences of a broader spectrum of inositol phosphates in microdissected brain tissue and to construct 3D-maps of these signaling compounds. The technique can be particularly well employed to investigate regional changes in the spectrum of higher inositol phosphates and phosphoinositides upon neuronal stimulation induced by motor activity or drug treatment.
M3 - SCORING: Zeitschriftenaufsatz
VL - 29
SP - 1887
EP - 1896
JO - NEUROCHEM RES
JF - NEUROCHEM RES
SN - 0364-3190
IS - 10
M1 - 10
ER -