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An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis

Standard

An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis. / Ernst, Nancy; Tiede, Stephan; Tronnier, Volker; Kruse, Charli; Zechel, Christina; Paus, Ralf.

in: EXP DERMATOL, Jahrgang 19, Nr. 6, 06.2010, S. 549-55.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ernst, N, Tiede, S, Tronnier, V, Kruse, C, Zechel, C & Paus, R 2010, 'An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis', EXP DERMATOL, Jg. 19, Nr. 6, S. 549-55. https://doi.org/10.1111/j.1600-0625.2009.01041.x

APA

Ernst, N., Tiede, S., Tronnier, V., Kruse, C., Zechel, C., & Paus, R. (2010). An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis. EXP DERMATOL, 19(6), 549-55. https://doi.org/10.1111/j.1600-0625.2009.01041.x

Vancouver

Ernst N, Tiede S, Tronnier V, Kruse C, Zechel C, Paus R. An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis. EXP DERMATOL. 2010 Jun;19(6):549-55. https://doi.org/10.1111/j.1600-0625.2009.01041.x

Bibtex

@article{ea4c77808e0d49fabf66142f9b29657c,
title = "An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis",
abstract = "Human skin-derived Nestin+ cells serve as a convenient source for autologous, adult, pluripotent progenitor cells that offer new therapeutic possibilities in cell-based regenerative medicine. However, the isolation of human Nestin+ cells has tended to be of very low efficiency and to produce highly variable cell yields. Here we report a standardised protocol that facilitates the isolation and enrichment of Nestin+ progenitor cells from enzymatically digested adult human scalp dermis. The use of distinct media like Dulbecco's modified Eagle medium supplemented with foetal bovine serum or, alternatively, serum-free, supplemented neural stem cell medium greatly affected cell morphology, proliferation and differentiation (e.g. towards a neural versus mesenchymal phenotype). Finally, Nestin+ cells were isolated from a heterogeneous dermis-derived progenitor cell population, which proliferates within clones or floating microspheres under defined serum-free culture conditions. Supplementation of the medium with epidermal growth factor and basic fibroblast growth factor as well as coating with fibronectin allowed the highest enrichment level of Nestin+ progenitors and differentiation towards neural fate. These methodological advances should greatly facilitate the isolation, culture and targeted differentiation of primary, adult human scalp skin dermis-derived Nestin+ cells.",
keywords = "Actins, Adult, Adult Stem Cells, Aged, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cell Separation, Culture Media, Culture Media, Serum-Free, Dermis, Female, Fibronectins, Gene Expression, Humans, Intermediate Filament Proteins, Male, Mesoderm, Middle Aged, Nerve Tissue Proteins, Nestin, Neurofilament Proteins, Neurons, Journal Article, Research Support, Non-U.S. Gov't",
author = "Nancy Ernst and Stephan Tiede and Volker Tronnier and Charli Kruse and Christina Zechel and Ralf Paus",
year = "2010",
month = jun,
doi = "10.1111/j.1600-0625.2009.01041.x",
language = "English",
volume = "19",
pages = "549--55",
journal = "EXP DERMATOL",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - An improved, standardised protocol for the isolation, enrichment and targeted neural differentiation of Nestin+ progenitors from adult human dermis

AU - Ernst, Nancy

AU - Tiede, Stephan

AU - Tronnier, Volker

AU - Kruse, Charli

AU - Zechel, Christina

AU - Paus, Ralf

PY - 2010/6

Y1 - 2010/6

N2 - Human skin-derived Nestin+ cells serve as a convenient source for autologous, adult, pluripotent progenitor cells that offer new therapeutic possibilities in cell-based regenerative medicine. However, the isolation of human Nestin+ cells has tended to be of very low efficiency and to produce highly variable cell yields. Here we report a standardised protocol that facilitates the isolation and enrichment of Nestin+ progenitor cells from enzymatically digested adult human scalp dermis. The use of distinct media like Dulbecco's modified Eagle medium supplemented with foetal bovine serum or, alternatively, serum-free, supplemented neural stem cell medium greatly affected cell morphology, proliferation and differentiation (e.g. towards a neural versus mesenchymal phenotype). Finally, Nestin+ cells were isolated from a heterogeneous dermis-derived progenitor cell population, which proliferates within clones or floating microspheres under defined serum-free culture conditions. Supplementation of the medium with epidermal growth factor and basic fibroblast growth factor as well as coating with fibronectin allowed the highest enrichment level of Nestin+ progenitors and differentiation towards neural fate. These methodological advances should greatly facilitate the isolation, culture and targeted differentiation of primary, adult human scalp skin dermis-derived Nestin+ cells.

AB - Human skin-derived Nestin+ cells serve as a convenient source for autologous, adult, pluripotent progenitor cells that offer new therapeutic possibilities in cell-based regenerative medicine. However, the isolation of human Nestin+ cells has tended to be of very low efficiency and to produce highly variable cell yields. Here we report a standardised protocol that facilitates the isolation and enrichment of Nestin+ progenitor cells from enzymatically digested adult human scalp dermis. The use of distinct media like Dulbecco's modified Eagle medium supplemented with foetal bovine serum or, alternatively, serum-free, supplemented neural stem cell medium greatly affected cell morphology, proliferation and differentiation (e.g. towards a neural versus mesenchymal phenotype). Finally, Nestin+ cells were isolated from a heterogeneous dermis-derived progenitor cell population, which proliferates within clones or floating microspheres under defined serum-free culture conditions. Supplementation of the medium with epidermal growth factor and basic fibroblast growth factor as well as coating with fibronectin allowed the highest enrichment level of Nestin+ progenitors and differentiation towards neural fate. These methodological advances should greatly facilitate the isolation, culture and targeted differentiation of primary, adult human scalp skin dermis-derived Nestin+ cells.

KW - Actins

KW - Adult

KW - Adult Stem Cells

KW - Aged

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cell Separation

KW - Culture Media

KW - Culture Media, Serum-Free

KW - Dermis

KW - Female

KW - Fibronectins

KW - Gene Expression

KW - Humans

KW - Intermediate Filament Proteins

KW - Male

KW - Mesoderm

KW - Middle Aged

KW - Nerve Tissue Proteins

KW - Nestin

KW - Neurofilament Proteins

KW - Neurons

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1600-0625.2009.01041.x

DO - 10.1111/j.1600-0625.2009.01041.x

M3 - SCORING: Journal article

C2 - 20100195

VL - 19

SP - 549

EP - 555

JO - EXP DERMATOL

JF - EXP DERMATOL

SN - 0906-6705

IS - 6

ER -