Altered growth factor requirements and cell cycle control in rat hepatoma cells versus adult rat hepatocytes in culture.
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Altered growth factor requirements and cell cycle control in rat hepatoma cells versus adult rat hepatocytes in culture. / Paul, D; Piasecki, Angelika; Baisch, H; Begemann, M.
in: EUR J CELL BIOL, Jahrgang 46, Nr. 2, 2, 1988, S. 270-274.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Altered growth factor requirements and cell cycle control in rat hepatoma cells versus adult rat hepatocytes in culture.
AU - Paul, D
AU - Piasecki, Angelika
AU - Baisch, H
AU - Begemann, M
PY - 1988
Y1 - 1988
N2 - Adult rat hepatocytes multiply in primary cultures when incubated in arginine-free MX-83 medium supplemented with dialyzed fetal calf serum, insulin, glucagon, hydrocortisone, epidermal growth factor, and transferrin. In the absence of mitogens, the fraction of the cells engaged in DNA synthesis dropped sharply. However, cells initiated DNA synthesis in response to the mitogenic mixture indicating that hepatocyte proliferation is controlled by G1----S transition rates. In contrast, rat hepatoma line DTH-3, derived from Morris 7777 "minimal deviation" hepatoma, required only insulin for proliferation in chemically defined MX-83 medium. The lengths of their cell cycle phases varied with the growth rate. The phases of the growth cycle were proportionately shortened (expanded) when the growth rate was increased (decreased). It is concluded that DTH-3 hepatoma cells, which display a decreased growth factor requirement as compared with adult rat hepatocytes differ from normal hepatocytes by fundamental alterations in the mechanisms controlling the progression of the cell cycle.
AB - Adult rat hepatocytes multiply in primary cultures when incubated in arginine-free MX-83 medium supplemented with dialyzed fetal calf serum, insulin, glucagon, hydrocortisone, epidermal growth factor, and transferrin. In the absence of mitogens, the fraction of the cells engaged in DNA synthesis dropped sharply. However, cells initiated DNA synthesis in response to the mitogenic mixture indicating that hepatocyte proliferation is controlled by G1----S transition rates. In contrast, rat hepatoma line DTH-3, derived from Morris 7777 "minimal deviation" hepatoma, required only insulin for proliferation in chemically defined MX-83 medium. The lengths of their cell cycle phases varied with the growth rate. The phases of the growth cycle were proportionately shortened (expanded) when the growth rate was increased (decreased). It is concluded that DTH-3 hepatoma cells, which display a decreased growth factor requirement as compared with adult rat hepatocytes differ from normal hepatocytes by fundamental alterations in the mechanisms controlling the progression of the cell cycle.
KW - Animals
KW - Cells, Cultured
KW - Rats
KW - Cell Cycle
KW - DNA/biosynthesis
KW - Rats, Inbred Strains
KW - Culture Media/pharmacology
KW - Growth Substances/physiology
KW - Insulin/pharmacology
KW - Liver/cytology
KW - Liver Neoplasms, Experimental/physiopathology/ultrastructure
KW - Tumor Cells, Cultured/drug effects/physiology/ultrastructure
KW - Animals
KW - Cells, Cultured
KW - Rats
KW - Cell Cycle
KW - DNA/biosynthesis
KW - Rats, Inbred Strains
KW - Culture Media/pharmacology
KW - Growth Substances/physiology
KW - Insulin/pharmacology
KW - Liver/cytology
KW - Liver Neoplasms, Experimental/physiopathology/ultrastructure
KW - Tumor Cells, Cultured/drug effects/physiology/ultrastructure
M3 - SCORING: Journal article
VL - 46
SP - 270
EP - 274
JO - EUR J CELL BIOL
JF - EUR J CELL BIOL
SN - 0171-9335
IS - 2
M1 - 2
ER -
