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Alphavbeta5-integrins mediate early steps of metastasis formation

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Alphavbeta5-integrins mediate early steps of metastasis formation. / Enns, Andreas; Korb, Timo; Schlüter, Kerstin; Gassmann, Peter; Spiegel, Hans-Ullrich; Senninger, Norbert; Mitjans, Francesc; Haier, Jörg.

in: EUR J CANCER, Jahrgang 41, Nr. 7, 05.2005, S. 1065-72.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Enns, A, Korb, T, Schlüter, K, Gassmann, P, Spiegel, H-U, Senninger, N, Mitjans, F & Haier, J 2005, 'Alphavbeta5-integrins mediate early steps of metastasis formation', EUR J CANCER, Jg. 41, Nr. 7, S. 1065-72. https://doi.org/10.1016/j.ejca.2004.12.031

APA

Enns, A., Korb, T., Schlüter, K., Gassmann, P., Spiegel, H-U., Senninger, N., Mitjans, F., & Haier, J. (2005). Alphavbeta5-integrins mediate early steps of metastasis formation. EUR J CANCER, 41(7), 1065-72. https://doi.org/10.1016/j.ejca.2004.12.031

Vancouver

Enns A, Korb T, Schlüter K, Gassmann P, Spiegel H-U, Senninger N et al. Alphavbeta5-integrins mediate early steps of metastasis formation. EUR J CANCER. 2005 Mai;41(7):1065-72. https://doi.org/10.1016/j.ejca.2004.12.031

Bibtex

@article{e96abfec8c7d4eeebf17ded72fcc7d5c,
title = "Alphavbeta5-integrins mediate early steps of metastasis formation",
abstract = "Tumour cell adhesion within the microvasculature of host organs, its stabilisation and cell invasion into the host organs, appear to be important steps in the formation of distant metastases. Intravital fluorescence-video microscopy was used to investigate the early steps in metastasis formation of colon carcinoma cells within the liver, which is the main target organ of colorectal carcinomas. The involvement of alphav-integrins was analysed in vivo using HT-29 cells after treatment with different function-blocking antibodies [pan-alphav (n=9 animals), specific alphavbeta3 (n=8 animals) and alphavbeta5 (n=8 animals)] or linear Arg-Gly-Asp (RGD)-containing peptides (RGD-peptides) (n=6 animals). Treatment with anti-alphav and anti-alphavbeta5 antibodies resulted in significantly (P<0.001) decreased tumour cell adhesion in vivo within the hepatic microvasculature. Cells treated with anti-alphavbeta3 antibodies or unspecific immunoglobulin-G (IgG) did not show significant changes in their adhesive properties. Furthermore, inhibition of cell adhesion was achieved by linear RGD-peptides in a dose-dependent manner. Relative numbers of migrated cells were not affected by any of the treatments. These results suggest that alphav-integrins, especially alphavbeta5, can influence the ability of circulating tumour cells to adhere within the hepatic microvessels. In contrast, migration of adherent cells into the liver parenchyma was not affected by alphav-integrin inhibition. Our findings support the hypothesis that specific interactions between circulating tumour cells and host organs are required for organ-specific tumour cell arrest.",
keywords = "Cell Adhesion, Colonic Neoplasms, Enzyme-Linked Immunosorbent Assay, HT29 Cells, Humans, Integrins, Liver Neoplasms, Neoplasm Invasiveness, Oligopeptides, Receptors, Vitronectin",
author = "Andreas Enns and Timo Korb and Kerstin Schl{\"u}ter and Peter Gassmann and Hans-Ullrich Spiegel and Norbert Senninger and Francesc Mitjans and J{\"o}rg Haier",
year = "2005",
month = may,
doi = "10.1016/j.ejca.2004.12.031",
language = "English",
volume = "41",
pages = "1065--72",
journal = "EUR J CANCER",
issn = "0959-8049",
publisher = "Elsevier Limited",
number = "7",

}

RIS

TY - JOUR

T1 - Alphavbeta5-integrins mediate early steps of metastasis formation

AU - Enns, Andreas

AU - Korb, Timo

AU - Schlüter, Kerstin

AU - Gassmann, Peter

AU - Spiegel, Hans-Ullrich

AU - Senninger, Norbert

AU - Mitjans, Francesc

AU - Haier, Jörg

PY - 2005/5

Y1 - 2005/5

N2 - Tumour cell adhesion within the microvasculature of host organs, its stabilisation and cell invasion into the host organs, appear to be important steps in the formation of distant metastases. Intravital fluorescence-video microscopy was used to investigate the early steps in metastasis formation of colon carcinoma cells within the liver, which is the main target organ of colorectal carcinomas. The involvement of alphav-integrins was analysed in vivo using HT-29 cells after treatment with different function-blocking antibodies [pan-alphav (n=9 animals), specific alphavbeta3 (n=8 animals) and alphavbeta5 (n=8 animals)] or linear Arg-Gly-Asp (RGD)-containing peptides (RGD-peptides) (n=6 animals). Treatment with anti-alphav and anti-alphavbeta5 antibodies resulted in significantly (P<0.001) decreased tumour cell adhesion in vivo within the hepatic microvasculature. Cells treated with anti-alphavbeta3 antibodies or unspecific immunoglobulin-G (IgG) did not show significant changes in their adhesive properties. Furthermore, inhibition of cell adhesion was achieved by linear RGD-peptides in a dose-dependent manner. Relative numbers of migrated cells were not affected by any of the treatments. These results suggest that alphav-integrins, especially alphavbeta5, can influence the ability of circulating tumour cells to adhere within the hepatic microvessels. In contrast, migration of adherent cells into the liver parenchyma was not affected by alphav-integrin inhibition. Our findings support the hypothesis that specific interactions between circulating tumour cells and host organs are required for organ-specific tumour cell arrest.

AB - Tumour cell adhesion within the microvasculature of host organs, its stabilisation and cell invasion into the host organs, appear to be important steps in the formation of distant metastases. Intravital fluorescence-video microscopy was used to investigate the early steps in metastasis formation of colon carcinoma cells within the liver, which is the main target organ of colorectal carcinomas. The involvement of alphav-integrins was analysed in vivo using HT-29 cells after treatment with different function-blocking antibodies [pan-alphav (n=9 animals), specific alphavbeta3 (n=8 animals) and alphavbeta5 (n=8 animals)] or linear Arg-Gly-Asp (RGD)-containing peptides (RGD-peptides) (n=6 animals). Treatment with anti-alphav and anti-alphavbeta5 antibodies resulted in significantly (P<0.001) decreased tumour cell adhesion in vivo within the hepatic microvasculature. Cells treated with anti-alphavbeta3 antibodies or unspecific immunoglobulin-G (IgG) did not show significant changes in their adhesive properties. Furthermore, inhibition of cell adhesion was achieved by linear RGD-peptides in a dose-dependent manner. Relative numbers of migrated cells were not affected by any of the treatments. These results suggest that alphav-integrins, especially alphavbeta5, can influence the ability of circulating tumour cells to adhere within the hepatic microvessels. In contrast, migration of adherent cells into the liver parenchyma was not affected by alphav-integrin inhibition. Our findings support the hypothesis that specific interactions between circulating tumour cells and host organs are required for organ-specific tumour cell arrest.

KW - Cell Adhesion

KW - Colonic Neoplasms

KW - Enzyme-Linked Immunosorbent Assay

KW - HT29 Cells

KW - Humans

KW - Integrins

KW - Liver Neoplasms

KW - Neoplasm Invasiveness

KW - Oligopeptides

KW - Receptors, Vitronectin

U2 - 10.1016/j.ejca.2004.12.031

DO - 10.1016/j.ejca.2004.12.031

M3 - SCORING: Journal article

C2 - 15862757

VL - 41

SP - 1065

EP - 1072

JO - EUR J CANCER

JF - EUR J CANCER

SN - 0959-8049

IS - 7

ER -