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All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals

Standard

All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals. / Bergs, Amelie C F; Liewald, Jana F; Rodriguez-Rozada, Silvia; Liu, Qiang; Wirt, Christin; Bessel, Artur; Zeitzschel, Nadja; Durmaz, Hilal; Nozownik, Adrianna; Dill, Holger; Jospin, Maëlle; Vierock, Johannes; Bargmann, Cornelia I; Hegemann, Peter; Wiegert, J Simon; Gottschalk, Alexander.

in: NAT COMMUN, Jahrgang 14, Nr. 1, 06.04.2023, S. 1939.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bergs, ACF, Liewald, JF, Rodriguez-Rozada, S, Liu, Q, Wirt, C, Bessel, A, Zeitzschel, N, Durmaz, H, Nozownik, A, Dill, H, Jospin, M, Vierock, J, Bargmann, CI, Hegemann, P, Wiegert, JS & Gottschalk, A 2023, 'All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals', NAT COMMUN, Jg. 14, Nr. 1, S. 1939. https://doi.org/10.1038/s41467-023-37622-6

APA

Bergs, A. C. F., Liewald, J. F., Rodriguez-Rozada, S., Liu, Q., Wirt, C., Bessel, A., Zeitzschel, N., Durmaz, H., Nozownik, A., Dill, H., Jospin, M., Vierock, J., Bargmann, C. I., Hegemann, P., Wiegert, J. S., & Gottschalk, A. (2023). All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals. NAT COMMUN, 14(1), 1939. https://doi.org/10.1038/s41467-023-37622-6

Vancouver

Bergs ACF, Liewald JF, Rodriguez-Rozada S, Liu Q, Wirt C, Bessel A et al. All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals. NAT COMMUN. 2023 Apr 6;14(1):1939. https://doi.org/10.1038/s41467-023-37622-6

Bibtex

@article{f77fa3219ddb4e0d844693979bc17955,
title = "All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals",
abstract = "Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits can quickly adapt, allowing perturbation, but not true control. Hence, we established an optogenetic voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional optogenetic actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping the cell to distinct potentials. We established the OVC in muscles and neurons of Caenorhabditis elegans, and transferred it to rat hippocampal neurons in slice culture. Fluorescence signals were calibrated to electrically measured potentials, and wavelengths to currents, enabling to determine optical I/V-relationships. The OVC reports on homeostatically altered cellular physiology in mutants and on Ca2+-channel properties, and can dynamically clamp spiking in C. elegans. Combining non-invasive imaging with control capabilities of electrophysiology, the OVC facilitates high-throughput, contact-less electrophysiology in individual cells and paves the way for true optogenetic control in behaving animals.",
keywords = "Animals, Rats, Caenorhabditis elegans/physiology, Action Potentials/physiology, Muscles, Neurons/physiology, Optogenetics/methods",
author = "Bergs, {Amelie C F} and Liewald, {Jana F} and Silvia Rodriguez-Rozada and Qiang Liu and Christin Wirt and Artur Bessel and Nadja Zeitzschel and Hilal Durmaz and Adrianna Nozownik and Holger Dill and Ma{\"e}lle Jospin and Johannes Vierock and Bargmann, {Cornelia I} and Peter Hegemann and Wiegert, {J Simon} and Alexander Gottschalk",
note = "{\textcopyright} 2023. The Author(s).",
year = "2023",
month = apr,
day = "6",
doi = "10.1038/s41467-023-37622-6",
language = "English",
volume = "14",
pages = "1939",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals

AU - Bergs, Amelie C F

AU - Liewald, Jana F

AU - Rodriguez-Rozada, Silvia

AU - Liu, Qiang

AU - Wirt, Christin

AU - Bessel, Artur

AU - Zeitzschel, Nadja

AU - Durmaz, Hilal

AU - Nozownik, Adrianna

AU - Dill, Holger

AU - Jospin, Maëlle

AU - Vierock, Johannes

AU - Bargmann, Cornelia I

AU - Hegemann, Peter

AU - Wiegert, J Simon

AU - Gottschalk, Alexander

N1 - © 2023. The Author(s).

PY - 2023/4/6

Y1 - 2023/4/6

N2 - Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits can quickly adapt, allowing perturbation, but not true control. Hence, we established an optogenetic voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional optogenetic actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping the cell to distinct potentials. We established the OVC in muscles and neurons of Caenorhabditis elegans, and transferred it to rat hippocampal neurons in slice culture. Fluorescence signals were calibrated to electrically measured potentials, and wavelengths to currents, enabling to determine optical I/V-relationships. The OVC reports on homeostatically altered cellular physiology in mutants and on Ca2+-channel properties, and can dynamically clamp spiking in C. elegans. Combining non-invasive imaging with control capabilities of electrophysiology, the OVC facilitates high-throughput, contact-less electrophysiology in individual cells and paves the way for true optogenetic control in behaving animals.

AB - Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits can quickly adapt, allowing perturbation, but not true control. Hence, we established an optogenetic voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional optogenetic actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping the cell to distinct potentials. We established the OVC in muscles and neurons of Caenorhabditis elegans, and transferred it to rat hippocampal neurons in slice culture. Fluorescence signals were calibrated to electrically measured potentials, and wavelengths to currents, enabling to determine optical I/V-relationships. The OVC reports on homeostatically altered cellular physiology in mutants and on Ca2+-channel properties, and can dynamically clamp spiking in C. elegans. Combining non-invasive imaging with control capabilities of electrophysiology, the OVC facilitates high-throughput, contact-less electrophysiology in individual cells and paves the way for true optogenetic control in behaving animals.

KW - Animals

KW - Rats

KW - Caenorhabditis elegans/physiology

KW - Action Potentials/physiology

KW - Muscles

KW - Neurons/physiology

KW - Optogenetics/methods

U2 - 10.1038/s41467-023-37622-6

DO - 10.1038/s41467-023-37622-6

M3 - SCORING: Journal article

C2 - 37024493

VL - 14

SP - 1939

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -