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Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes

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Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes. / Herraiz-Martínez, Adela; Álvarez-García, Jesus; Llach, Anna; Molina, Cristina E; Fernandes, Jacqueline; Ferrero-Gregori, Andreu; Rodríguez, Cristina; Vallmitjana, Alexander; Benítez, Raúl; Padró, Josep M; Martínez-González, José; Cinca, Juan; Hove-Madsen, Leif.

in: CARDIOVASC RES, Jahrgang 106, Nr. 1, 01.04.2015, S. 76-86.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Herraiz-Martínez, A, Álvarez-García, J, Llach, A, Molina, CE, Fernandes, J, Ferrero-Gregori, A, Rodríguez, C, Vallmitjana, A, Benítez, R, Padró, JM, Martínez-González, J, Cinca, J & Hove-Madsen, L 2015, 'Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes', CARDIOVASC RES, Jg. 106, Nr. 1, S. 76-86. https://doi.org/10.1093/cvr/cvv046

APA

Herraiz-Martínez, A., Álvarez-García, J., Llach, A., Molina, C. E., Fernandes, J., Ferrero-Gregori, A., Rodríguez, C., Vallmitjana, A., Benítez, R., Padró, J. M., Martínez-González, J., Cinca, J., & Hove-Madsen, L. (2015). Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes. CARDIOVASC RES, 106(1), 76-86. https://doi.org/10.1093/cvr/cvv046

Vancouver

Herraiz-Martínez A, Álvarez-García J, Llach A, Molina CE, Fernandes J, Ferrero-Gregori A et al. Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes. CARDIOVASC RES. 2015 Apr 1;106(1):76-86. https://doi.org/10.1093/cvr/cvv046

Bibtex

@article{ab690d52b6004f4db60413a3e435356d,
title = "Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes",
abstract = "AIMS: Ageing-related cardiac disorders such as heart failure and atrial fibrillation often present with intracellular calcium homeostasis dysfunction. However, knowledge of the intrinsic effects of ageing on cellular calcium handling in the human heart is sparse. Therefore, this study aimed to analyse how ageing affects key mechanisms that regulate intracellular calcium in human atrial myocytes.METHODS AND RESULTS: Whole membrane currents and intracellular calcium transients were measured in isolated human right atrial myocytes from 80 patients with normal left atrial dimensions and no history of atrial fibrillation. Patients were categorized as young (<55 years, n = 21), middle aged (55-74 years, n = 42), and old (≥75 years, n = 17). Protein levels were determined by western blot. Ageing was associated with the following electrophysiological changes: (i) a 3.2-fold decrease in the calcium transient (P < 0.01); (ii) reduction of the L-type calcium current (ICa) amplitude (2.4 ± 0.3 pA/pF vs. 1.4 ± 0.2 pA/pF, P < 0.01); (iii) lower levels of L-type calcium channel alpha-subunit (P < 0.05); (iv) lower rates of both fast (14.5 ± 0.9 ms vs. 20.9 ± 1.9, P < 0.01) and slow (73 ± 3 vs. 120 ± 12 ms, P < 0.001) ICa inactivation; and (v) a decrease in the sarcoplasmic reticulum calcium content (10.1 ± 0.8 vs. 6.4 ± 0.6 amol/pF, P < 0.005) associated with a significant decrease in both SERCA2 (P < 0.05) and calsequestrin-2 (P < 0.05) protein levels. In contrast, ageing did not affect spontaneous sarcoplasmic reticulum calcium release.CONCLUSION: Ageing is associated with depression of SR calcium content, L-type calcium current, and calcium transient amplitude that may favour a progressive decline in right atrial contractile function with age.",
keywords = "Adult, Aged, Aged, 80 and over, Aging, Calcium, Calcium Channels, L-Type, Calsequestrin, Female, Heart Atria, Homeostasis, Humans, Male, Middle Aged, Myocardial Contraction, Myocytes, Cardiac, Patch-Clamp Techniques, Sarcoplasmic Reticulum, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "Adela Herraiz-Mart{\'i}nez and Jesus {\'A}lvarez-Garc{\'i}a and Anna Llach and Molina, {Cristina E} and Jacqueline Fernandes and Andreu Ferrero-Gregori and Cristina Rodr{\'i}guez and Alexander Vallmitjana and Ra{\'u}l Ben{\'i}tez and Padr{\'o}, {Josep M} and Jos{\'e} Mart{\'i}nez-Gonz{\'a}lez and Juan Cinca and Leif Hove-Madsen",
note = "{\textcopyright} The Author 2015. Published by Oxford University Press on behalf of the European Society of Cardiology.",
year = "2015",
month = apr,
day = "1",
doi = "10.1093/cvr/cvv046",
language = "English",
volume = "106",
pages = "76--86",
journal = "CARDIOVASC RES",
issn = "0008-6363",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes

AU - Herraiz-Martínez, Adela

AU - Álvarez-García, Jesus

AU - Llach, Anna

AU - Molina, Cristina E

AU - Fernandes, Jacqueline

AU - Ferrero-Gregori, Andreu

AU - Rodríguez, Cristina

AU - Vallmitjana, Alexander

AU - Benítez, Raúl

AU - Padró, Josep M

AU - Martínez-González, José

AU - Cinca, Juan

AU - Hove-Madsen, Leif

N1 - © The Author 2015. Published by Oxford University Press on behalf of the European Society of Cardiology.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - AIMS: Ageing-related cardiac disorders such as heart failure and atrial fibrillation often present with intracellular calcium homeostasis dysfunction. However, knowledge of the intrinsic effects of ageing on cellular calcium handling in the human heart is sparse. Therefore, this study aimed to analyse how ageing affects key mechanisms that regulate intracellular calcium in human atrial myocytes.METHODS AND RESULTS: Whole membrane currents and intracellular calcium transients were measured in isolated human right atrial myocytes from 80 patients with normal left atrial dimensions and no history of atrial fibrillation. Patients were categorized as young (<55 years, n = 21), middle aged (55-74 years, n = 42), and old (≥75 years, n = 17). Protein levels were determined by western blot. Ageing was associated with the following electrophysiological changes: (i) a 3.2-fold decrease in the calcium transient (P < 0.01); (ii) reduction of the L-type calcium current (ICa) amplitude (2.4 ± 0.3 pA/pF vs. 1.4 ± 0.2 pA/pF, P < 0.01); (iii) lower levels of L-type calcium channel alpha-subunit (P < 0.05); (iv) lower rates of both fast (14.5 ± 0.9 ms vs. 20.9 ± 1.9, P < 0.01) and slow (73 ± 3 vs. 120 ± 12 ms, P < 0.001) ICa inactivation; and (v) a decrease in the sarcoplasmic reticulum calcium content (10.1 ± 0.8 vs. 6.4 ± 0.6 amol/pF, P < 0.005) associated with a significant decrease in both SERCA2 (P < 0.05) and calsequestrin-2 (P < 0.05) protein levels. In contrast, ageing did not affect spontaneous sarcoplasmic reticulum calcium release.CONCLUSION: Ageing is associated with depression of SR calcium content, L-type calcium current, and calcium transient amplitude that may favour a progressive decline in right atrial contractile function with age.

AB - AIMS: Ageing-related cardiac disorders such as heart failure and atrial fibrillation often present with intracellular calcium homeostasis dysfunction. However, knowledge of the intrinsic effects of ageing on cellular calcium handling in the human heart is sparse. Therefore, this study aimed to analyse how ageing affects key mechanisms that regulate intracellular calcium in human atrial myocytes.METHODS AND RESULTS: Whole membrane currents and intracellular calcium transients were measured in isolated human right atrial myocytes from 80 patients with normal left atrial dimensions and no history of atrial fibrillation. Patients were categorized as young (<55 years, n = 21), middle aged (55-74 years, n = 42), and old (≥75 years, n = 17). Protein levels were determined by western blot. Ageing was associated with the following electrophysiological changes: (i) a 3.2-fold decrease in the calcium transient (P < 0.01); (ii) reduction of the L-type calcium current (ICa) amplitude (2.4 ± 0.3 pA/pF vs. 1.4 ± 0.2 pA/pF, P < 0.01); (iii) lower levels of L-type calcium channel alpha-subunit (P < 0.05); (iv) lower rates of both fast (14.5 ± 0.9 ms vs. 20.9 ± 1.9, P < 0.01) and slow (73 ± 3 vs. 120 ± 12 ms, P < 0.001) ICa inactivation; and (v) a decrease in the sarcoplasmic reticulum calcium content (10.1 ± 0.8 vs. 6.4 ± 0.6 amol/pF, P < 0.005) associated with a significant decrease in both SERCA2 (P < 0.05) and calsequestrin-2 (P < 0.05) protein levels. In contrast, ageing did not affect spontaneous sarcoplasmic reticulum calcium release.CONCLUSION: Ageing is associated with depression of SR calcium content, L-type calcium current, and calcium transient amplitude that may favour a progressive decline in right atrial contractile function with age.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Aging

KW - Calcium

KW - Calcium Channels, L-Type

KW - Calsequestrin

KW - Female

KW - Heart Atria

KW - Homeostasis

KW - Humans

KW - Male

KW - Middle Aged

KW - Myocardial Contraction

KW - Myocytes, Cardiac

KW - Patch-Clamp Techniques

KW - Sarcoplasmic Reticulum

KW - Sarcoplasmic Reticulum Calcium-Transporting ATPases

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1093/cvr/cvv046

DO - 10.1093/cvr/cvv046

M3 - SCORING: Journal article

C2 - 25712961

VL - 106

SP - 76

EP - 86

JO - CARDIOVASC RES

JF - CARDIOVASC RES

SN - 0008-6363

IS - 1

ER -