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Adhesion of small cell lung cancer cells to E- and P-selectin under physiological flow conditions: implications for metastasis formation.

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Adhesion of small cell lung cancer cells to E- and P-selectin under physiological flow conditions: implications for metastasis formation. / Richter, Ulrich; Schröder, Christine; Wicklein, Daniel; Lange, Tobias; Geleff, Silvana; Dippel, Virginia; Schumacher, Udo; Klutmann, Susanne.

in: HISTOCHEM CELL BIOL, Jahrgang 135, Nr. 5, 5, 2011, S. 499-512.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Richter, U, Schröder, C, Wicklein, D, Lange, T, Geleff, S, Dippel, V, Schumacher, U & Klutmann, S 2011, 'Adhesion of small cell lung cancer cells to E- and P-selectin under physiological flow conditions: implications for metastasis formation.', HISTOCHEM CELL BIOL, Jg. 135, Nr. 5, 5, S. 499-512. <http://www.ncbi.nlm.nih.gov/pubmed/21544708?dopt=Citation>

APA

Richter, U., Schröder, C., Wicklein, D., Lange, T., Geleff, S., Dippel, V., Schumacher, U., & Klutmann, S. (2011). Adhesion of small cell lung cancer cells to E- and P-selectin under physiological flow conditions: implications for metastasis formation. HISTOCHEM CELL BIOL, 135(5), 499-512. [5]. http://www.ncbi.nlm.nih.gov/pubmed/21544708?dopt=Citation

Vancouver

Richter U, Schröder C, Wicklein D, Lange T, Geleff S, Dippel V et al. Adhesion of small cell lung cancer cells to E- and P-selectin under physiological flow conditions: implications for metastasis formation. HISTOCHEM CELL BIOL. 2011;135(5):499-512. 5.

Bibtex

@article{826554e86d5f4f65ab468bd5f70c8de4,
title = "Adhesion of small cell lung cancer cells to E- and P-selectin under physiological flow conditions: implications for metastasis formation.",
abstract = "Haematogenous metastasis of small cell lung cancer (SCLC) is still a poorly understood process and represents the life threatening event in this malignancy. In particular, the rate-limiting step within the metastatic cascade is not yet clearly defined although, many findings indicate, that extravasation of circulating tumour cells is crucially important as most tumour cells within the circulation undergo apoptosis. If extravasation of SCLC tumour cells mimics leukocyte-endothelial interactions, SCLC cells should adhere to E- and P-selectins expressed on the luminal surface of activated endothelium. The adhesion to E- and P-selectin under physiological shear stress with regard to adhesive events, rolling behaviour and rolling velocity was determined in the human SCLC cell lines SW2, H69, H82, OH1 and OH3. OH1 SCLC cells adhered best to recombinant human (rh) E-selectin FC-chimeras and human lung endothelial cells (HPMEC), H82 SCLC cells adhered best to activated human umbilical vein endothelial cells (HUVEC) under physiological shear stress. As OH1 cells had also produced by far the highest number of spontaneous lung metastases when xenografted into pfp/rag2 mice in previous experiments our findings implicate that adhesion of SCLC cells to E-selectin is of paramount importance in SCLC metastasis formation.",
keywords = "Humans, Immunohistochemistry, Flow Cytometry, Tumor Cells, Cultured, Cell Adhesion, E-Selectin/*metabolism, Endothelial Cells/metabolism/pathology, Lung Neoplasms/*pathology, Neoplasm Metastasis/*pathology, P-Selectin/*metabolism, Recombinant Proteins/metabolism, Small Cell Lung Carcinoma/*pathology, Humans, Immunohistochemistry, Flow Cytometry, Tumor Cells, Cultured, Cell Adhesion, E-Selectin/*metabolism, Endothelial Cells/metabolism/pathology, Lung Neoplasms/*pathology, Neoplasm Metastasis/*pathology, P-Selectin/*metabolism, Recombinant Proteins/metabolism, Small Cell Lung Carcinoma/*pathology",
author = "Ulrich Richter and Christine Schr{\"o}der and Daniel Wicklein and Tobias Lange and Silvana Geleff and Virginia Dippel and Udo Schumacher and Susanne Klutmann",
year = "2011",
language = "English",
volume = "135",
pages = "499--512",
journal = "HISTOCHEM CELL BIOL",
issn = "0948-6143",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - Adhesion of small cell lung cancer cells to E- and P-selectin under physiological flow conditions: implications for metastasis formation.

AU - Richter, Ulrich

AU - Schröder, Christine

AU - Wicklein, Daniel

AU - Lange, Tobias

AU - Geleff, Silvana

AU - Dippel, Virginia

AU - Schumacher, Udo

AU - Klutmann, Susanne

PY - 2011

Y1 - 2011

N2 - Haematogenous metastasis of small cell lung cancer (SCLC) is still a poorly understood process and represents the life threatening event in this malignancy. In particular, the rate-limiting step within the metastatic cascade is not yet clearly defined although, many findings indicate, that extravasation of circulating tumour cells is crucially important as most tumour cells within the circulation undergo apoptosis. If extravasation of SCLC tumour cells mimics leukocyte-endothelial interactions, SCLC cells should adhere to E- and P-selectins expressed on the luminal surface of activated endothelium. The adhesion to E- and P-selectin under physiological shear stress with regard to adhesive events, rolling behaviour and rolling velocity was determined in the human SCLC cell lines SW2, H69, H82, OH1 and OH3. OH1 SCLC cells adhered best to recombinant human (rh) E-selectin FC-chimeras and human lung endothelial cells (HPMEC), H82 SCLC cells adhered best to activated human umbilical vein endothelial cells (HUVEC) under physiological shear stress. As OH1 cells had also produced by far the highest number of spontaneous lung metastases when xenografted into pfp/rag2 mice in previous experiments our findings implicate that adhesion of SCLC cells to E-selectin is of paramount importance in SCLC metastasis formation.

AB - Haematogenous metastasis of small cell lung cancer (SCLC) is still a poorly understood process and represents the life threatening event in this malignancy. In particular, the rate-limiting step within the metastatic cascade is not yet clearly defined although, many findings indicate, that extravasation of circulating tumour cells is crucially important as most tumour cells within the circulation undergo apoptosis. If extravasation of SCLC tumour cells mimics leukocyte-endothelial interactions, SCLC cells should adhere to E- and P-selectins expressed on the luminal surface of activated endothelium. The adhesion to E- and P-selectin under physiological shear stress with regard to adhesive events, rolling behaviour and rolling velocity was determined in the human SCLC cell lines SW2, H69, H82, OH1 and OH3. OH1 SCLC cells adhered best to recombinant human (rh) E-selectin FC-chimeras and human lung endothelial cells (HPMEC), H82 SCLC cells adhered best to activated human umbilical vein endothelial cells (HUVEC) under physiological shear stress. As OH1 cells had also produced by far the highest number of spontaneous lung metastases when xenografted into pfp/rag2 mice in previous experiments our findings implicate that adhesion of SCLC cells to E-selectin is of paramount importance in SCLC metastasis formation.

KW - Humans

KW - Immunohistochemistry

KW - Flow Cytometry

KW - Tumor Cells, Cultured

KW - Cell Adhesion

KW - E-Selectin/metabolism

KW - Endothelial Cells/metabolism/pathology

KW - Lung Neoplasms/pathology

KW - Neoplasm Metastasis/pathology

KW - P-Selectin/metabolism

KW - Recombinant Proteins/metabolism

KW - Small Cell Lung Carcinoma/pathology

KW - Humans

KW - Immunohistochemistry

KW - Flow Cytometry

KW - Tumor Cells, Cultured

KW - Cell Adhesion

KW - E-Selectin/metabolism

KW - Endothelial Cells/metabolism/pathology

KW - Lung Neoplasms/pathology

KW - Neoplasm Metastasis/pathology

KW - P-Selectin/metabolism

KW - Recombinant Proteins/metabolism

KW - Small Cell Lung Carcinoma/pathology

M3 - SCORING: Journal article

VL - 135

SP - 499

EP - 512

JO - HISTOCHEM CELL BIOL

JF - HISTOCHEM CELL BIOL

SN - 0948-6143

IS - 5

M1 - 5

ER -