Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease

Anna K Lundberg; Lena Jonasson; Göran K Hansson; Reiner K W Mailer

doi:10.1016/j.atherosclerosis.2017.10.026

Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease

Standard

Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease. / Lundberg, Anna K; Jonasson, Lena; Hansson, Göran K; Mailer, Reiner K W.

in: ATHEROSCLEROSIS, Jahrgang 267, 12.2017, S. 27-33.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Lundberg, AK, Jonasson, L, Hansson, GK & Mailer, RKW 2017, 'Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease', ATHEROSCLEROSIS, Jg. 267, S. 27-33. https://doi.org/10.1016/j.atherosclerosis.2017.10.026

APA

Lundberg, A. K., Jonasson, L., Hansson, G. K., & Mailer, R. K. W. (2017). Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease. ATHEROSCLEROSIS, 267, 27-33. https://doi.org/10.1016/j.atherosclerosis.2017.10.026

Vancouver

Lundberg AK, Jonasson L, Hansson GK, Mailer RKW. Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease. ATHEROSCLEROSIS. 2017 Dez;267:27-33. https://doi.org/10.1016/j.atherosclerosis.2017.10.026

Bibtex

@article{9a1b2799b3f744d796f150674971ed17,

title = "Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease",

abstract = "BACKGROUND AND AIMS: The expression of FOXP3 isoforms affects regulatory T (Treg) cell function. Reduced Treg cell function has been associated with coronary artery disease (CAD). However, alternative splicing of FOXP3 in CAD has not been investigated.METHODS: FOXP3 splice variants and IL17A transcripts in peripheral blood mononuclear cells from stable CAD patients and healthy controls were quantified, and FOXP3 isoform expression in response to T cell receptor (TCR) stimulation or LDL was analyzed by flow cytometry.RESULTS: Compared to healthy controls, CAD patients expressed significantly more FOXP3 transcripts that included exon 2, whereas alternative splicing of exon 7 in correlation with IL17A expression was reduced. Moreover, TCR stimulation, as well as exposure to LDL, decreased alternative splicing of FOXP3 in CD4+ T cells in vitro.CONCLUSIONS: Our results demonstrate that blood mononuclear cells in stable CAD patients express a ratio of FOXP3 isoforms that is characteristic for activated CD4+ T cells.",

keywords = "Journal Article",

author = "Lundberg, {Anna K} and Lena Jonasson and Hansson, {G{\"o}ran K} and Mailer, {Reiner K W}",

year = "2017",

month = dec,

doi = "10.1016/j.atherosclerosis.2017.10.026",

language = "English",

volume = "267",

pages = "27--33",

journal = "ATHEROSCLEROSIS",

issn = "0021-9150",

publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - Activation-induced FOXP3 isoform profile in peripheral CD4+ T cells is associated with coronary artery disease

AU - Lundberg, Anna K

AU - Jonasson, Lena

AU - Hansson, Göran K

AU - Mailer, Reiner K W

PY - 2017/12

Y1 - 2017/12

N2 - BACKGROUND AND AIMS: The expression of FOXP3 isoforms affects regulatory T (Treg) cell function. Reduced Treg cell function has been associated with coronary artery disease (CAD). However, alternative splicing of FOXP3 in CAD has not been investigated.METHODS: FOXP3 splice variants and IL17A transcripts in peripheral blood mononuclear cells from stable CAD patients and healthy controls were quantified, and FOXP3 isoform expression in response to T cell receptor (TCR) stimulation or LDL was analyzed by flow cytometry.RESULTS: Compared to healthy controls, CAD patients expressed significantly more FOXP3 transcripts that included exon 2, whereas alternative splicing of exon 7 in correlation with IL17A expression was reduced. Moreover, TCR stimulation, as well as exposure to LDL, decreased alternative splicing of FOXP3 in CD4+ T cells in vitro.CONCLUSIONS: Our results demonstrate that blood mononuclear cells in stable CAD patients express a ratio of FOXP3 isoforms that is characteristic for activated CD4+ T cells.

AB - BACKGROUND AND AIMS: The expression of FOXP3 isoforms affects regulatory T (Treg) cell function. Reduced Treg cell function has been associated with coronary artery disease (CAD). However, alternative splicing of FOXP3 in CAD has not been investigated.METHODS: FOXP3 splice variants and IL17A transcripts in peripheral blood mononuclear cells from stable CAD patients and healthy controls were quantified, and FOXP3 isoform expression in response to T cell receptor (TCR) stimulation or LDL was analyzed by flow cytometry.RESULTS: Compared to healthy controls, CAD patients expressed significantly more FOXP3 transcripts that included exon 2, whereas alternative splicing of exon 7 in correlation with IL17A expression was reduced. Moreover, TCR stimulation, as well as exposure to LDL, decreased alternative splicing of FOXP3 in CD4+ T cells in vitro.CONCLUSIONS: Our results demonstrate that blood mononuclear cells in stable CAD patients express a ratio of FOXP3 isoforms that is characteristic for activated CD4+ T cells.

KW - Journal Article

U2 - 10.1016/j.atherosclerosis.2017.10.026

DO - 10.1016/j.atherosclerosis.2017.10.026

M3 - SCORING: Journal article

C2 - 29100058

VL - 267

SP - 27

EP - 33

JO - ATHEROSCLEROSIS

JF - ATHEROSCLEROSIS

SN - 0021-9150

ER -