Absence of mouse pleiotrophin does not affect bone formation in vivo.
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Absence of mouse pleiotrophin does not affect bone formation in vivo. / Lehmann, Wolfgang; Schinke, Thorsten; Schilling, Arndt; Catalá-Lehnen, Philip; Gebauer, Matthias; Pogoda, Pia; Gerstenfeld, Louis C; Rueger, Johannes M; Einhorn, Thomas A; Amling, Michael.
in: BONE, Jahrgang 35, Nr. 6, 6, 2004, S. 1247-1255.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Absence of mouse pleiotrophin does not affect bone formation in vivo.
AU - Lehmann, Wolfgang
AU - Schinke, Thorsten
AU - Schilling, Arndt
AU - Catalá-Lehnen, Philip
AU - Gebauer, Matthias
AU - Pogoda, Pia
AU - Gerstenfeld, Louis C
AU - Rueger, Johannes M
AU - Einhorn, Thomas A
AU - Amling, Michael
PY - 2004
Y1 - 2004
N2 - Pleiotrophin (Ptn) is an extracellular matrix protein that regulates hippocampal synaptic plasticity and learning behavior in vivo. Since the overexpression of Ptn in transgenic mice leads to increased bone formation, we analyzed whether a deficiency in Ptn expression would have a negative effect on bone remodeling. Bones from Ptn-deficient mice and wild-type littermates were analyzed using radiography, muCT imaging and undecalcified histology. Biomechanical stability was determined in a three-point-bending assay. Cellular activities were assessed using dynamic histomorphometry and the determination of urinary collagen degradation products. Skeletons of Ptn-deficient mice have no gross abnormalities, displayed a normal size, and showed no differences in growth plate organization compared to wild-type littermates. There were no obvious differences in bone mass as determined by radiographic and muCT imaging. The absence of a bone remodeling phenotype in Ptn-deficient mice was further confirmed using static histomorphometry and biomechanical testing. Finally, the number, morphology, and function of osteoclasts, osteoblasts, and osteocytes were not altered in Ptn-deficient mice compared to wild-type littermates. The complete skeletal analysis of Ptn-deficient mice presented here demonstrates that the lack of Ptn in mice does not affect bone formation in vivo. Therefore, Ptn does not play a significant role in normal bone physiology.
AB - Pleiotrophin (Ptn) is an extracellular matrix protein that regulates hippocampal synaptic plasticity and learning behavior in vivo. Since the overexpression of Ptn in transgenic mice leads to increased bone formation, we analyzed whether a deficiency in Ptn expression would have a negative effect on bone remodeling. Bones from Ptn-deficient mice and wild-type littermates were analyzed using radiography, muCT imaging and undecalcified histology. Biomechanical stability was determined in a three-point-bending assay. Cellular activities were assessed using dynamic histomorphometry and the determination of urinary collagen degradation products. Skeletons of Ptn-deficient mice have no gross abnormalities, displayed a normal size, and showed no differences in growth plate organization compared to wild-type littermates. There were no obvious differences in bone mass as determined by radiographic and muCT imaging. The absence of a bone remodeling phenotype in Ptn-deficient mice was further confirmed using static histomorphometry and biomechanical testing. Finally, the number, morphology, and function of osteoclasts, osteoblasts, and osteocytes were not altered in Ptn-deficient mice compared to wild-type littermates. The complete skeletal analysis of Ptn-deficient mice presented here demonstrates that the lack of Ptn in mice does not affect bone formation in vivo. Therefore, Ptn does not play a significant role in normal bone physiology.
M3 - SCORING: Zeitschriftenaufsatz
VL - 35
SP - 1247
EP - 1255
JO - BONE
JF - BONE
SN - 8756-3282
IS - 6
M1 - 6
ER -
