A Versatile Microarray Platform for Capturing Rare Cells
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A Versatile Microarray Platform for Capturing Rare Cells. / Brinkmann , Falko ; Hirtz, Michael; Haller, Anna; Gorges, Tobias M; Vellekoop, Michael J; Riethdorf, Sabine; Müller, Volkmar; Pantel, Klaus; Fuchs, Harald.
in: SCI REP-UK, Jahrgang 5, 23.10.2015, S. Art. 15342.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A Versatile Microarray Platform for Capturing Rare Cells
AU - Brinkmann , Falko
AU - Hirtz, Michael
AU - Haller, Anna
AU - Gorges, Tobias M
AU - Vellekoop, Michael J
AU - Riethdorf, Sabine
AU - Müller, Volkmar
AU - Pantel, Klaus
AU - Fuchs, Harald
PY - 2015/10/23
Y1 - 2015/10/23
N2 - Analyses of rare events occurring at extremely low frequencies in body fluids are still challenging. We established a versatile microarray-based platform able to capture single target cells from large background populations. As use case we chose the challenging application of detecting circulating tumor cells (CTCs) - about one cell in a billion normal blood cells. After incubation with an antibody cocktail, targeted cells are extracted on a microarray in a microfluidic chip. The accessibility of our platform allows for subsequent recovery of targets for further analysis. The microarray facilitates exclusion of false positive capture events by co-localization allowing for detection without fluorescent labelling. Analyzing blood samples from cancer patients with our platform reached and partly outreached gold standard performance, demonstrating feasibility for clinical application. Clinical researchers free choice of antibody cocktail without need for altered chip manufacturing or incubation protocol, allows virtual arbitrary targeting of capture species and therefore wide spread applications in biomedical sciences.
AB - Analyses of rare events occurring at extremely low frequencies in body fluids are still challenging. We established a versatile microarray-based platform able to capture single target cells from large background populations. As use case we chose the challenging application of detecting circulating tumor cells (CTCs) - about one cell in a billion normal blood cells. After incubation with an antibody cocktail, targeted cells are extracted on a microarray in a microfluidic chip. The accessibility of our platform allows for subsequent recovery of targets for further analysis. The microarray facilitates exclusion of false positive capture events by co-localization allowing for detection without fluorescent labelling. Analyzing blood samples from cancer patients with our platform reached and partly outreached gold standard performance, demonstrating feasibility for clinical application. Clinical researchers free choice of antibody cocktail without need for altered chip manufacturing or incubation protocol, allows virtual arbitrary targeting of capture species and therefore wide spread applications in biomedical sciences.
U2 - 10.1038/srep15342
DO - 10.1038/srep15342
M3 - SCORING: Journal article
C2 - 26493176
VL - 5
SP - Art. 15342
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
ER -