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A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein

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A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein. / Dusart, Philip; Fagerberg, Linn; Perisic, Ljubica; Civelek, Mete; Struck, Eike; Hedin, Ulf; Uhlén, Mathias; Trégouët, David-Alexandre; Renné, Thomas; Odeberg, Jacob; Butler, Lynn M.

in: SCI REP-UK, Jahrgang 8, Nr. 1, 02.10.2018, S. 14668.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Dusart, P, Fagerberg, L, Perisic, L, Civelek, M, Struck, E, Hedin, U, Uhlén, M, Trégouët, D-A, Renné, T, Odeberg, J & Butler, LM 2018, 'A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein', SCI REP-UK, Jg. 8, Nr. 1, S. 14668. https://doi.org/10.1038/s41598-018-32859-4

APA

Dusart, P., Fagerberg, L., Perisic, L., Civelek, M., Struck, E., Hedin, U., Uhlén, M., Trégouët, D-A., Renné, T., Odeberg, J., & Butler, L. M. (2018). A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein. SCI REP-UK, 8(1), 14668. https://doi.org/10.1038/s41598-018-32859-4

Vancouver

Dusart P, Fagerberg L, Perisic L, Civelek M, Struck E, Hedin U et al. A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein. SCI REP-UK. 2018 Okt 2;8(1):14668. https://doi.org/10.1038/s41598-018-32859-4

Bibtex

@article{f25e4e3ccc354a37a901a089d64578b2,
title = "A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein",
abstract = "The intermediate filament protein nestin is expressed during embryonic development, but considered largely restricted to areas of regeneration in the adult. Here, we perform a body-wide transcriptome and protein-profiling analysis to reveal that nestin is constitutively, and highly-selectively, expressed in adult human endothelial cells (EC), independent of proliferative status. Correspondingly, we demonstrate that it is not a marker for tumour EC in multiple malignancy types. Imaging of EC from different vascular beds reveals nestin subcellular distribution is shear-modulated. siRNA inhibition of nestin increases EC proliferation, and nestin expression is reduced in atherosclerotic plaque neovessels. eQTL analysis reveals an association between SNPs linked to cardiovascular disease and reduced aortic EC nestin mRNA expression. Our study challenges the dogma that nestin is a marker of proliferation, and provides insight into its regulation and function in EC. Furthermore, our systems-based approach can be applied to investigate body-wide expression profiles of any candidate protein.",
keywords = "Journal Article",
author = "Philip Dusart and Linn Fagerberg and Ljubica Perisic and Mete Civelek and Eike Struck and Ulf Hedin and Mathias Uhl{\'e}n and David-Alexandre Tr{\'e}gou{\"e}t and Thomas Renn{\'e} and Jacob Odeberg and Butler, {Lynn M}",
year = "2018",
month = oct,
day = "2",
doi = "10.1038/s41598-018-32859-4",
language = "English",
volume = "8",
pages = "14668",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein

AU - Dusart, Philip

AU - Fagerberg, Linn

AU - Perisic, Ljubica

AU - Civelek, Mete

AU - Struck, Eike

AU - Hedin, Ulf

AU - Uhlén, Mathias

AU - Trégouët, David-Alexandre

AU - Renné, Thomas

AU - Odeberg, Jacob

AU - Butler, Lynn M

PY - 2018/10/2

Y1 - 2018/10/2

N2 - The intermediate filament protein nestin is expressed during embryonic development, but considered largely restricted to areas of regeneration in the adult. Here, we perform a body-wide transcriptome and protein-profiling analysis to reveal that nestin is constitutively, and highly-selectively, expressed in adult human endothelial cells (EC), independent of proliferative status. Correspondingly, we demonstrate that it is not a marker for tumour EC in multiple malignancy types. Imaging of EC from different vascular beds reveals nestin subcellular distribution is shear-modulated. siRNA inhibition of nestin increases EC proliferation, and nestin expression is reduced in atherosclerotic plaque neovessels. eQTL analysis reveals an association between SNPs linked to cardiovascular disease and reduced aortic EC nestin mRNA expression. Our study challenges the dogma that nestin is a marker of proliferation, and provides insight into its regulation and function in EC. Furthermore, our systems-based approach can be applied to investigate body-wide expression profiles of any candidate protein.

AB - The intermediate filament protein nestin is expressed during embryonic development, but considered largely restricted to areas of regeneration in the adult. Here, we perform a body-wide transcriptome and protein-profiling analysis to reveal that nestin is constitutively, and highly-selectively, expressed in adult human endothelial cells (EC), independent of proliferative status. Correspondingly, we demonstrate that it is not a marker for tumour EC in multiple malignancy types. Imaging of EC from different vascular beds reveals nestin subcellular distribution is shear-modulated. siRNA inhibition of nestin increases EC proliferation, and nestin expression is reduced in atherosclerotic plaque neovessels. eQTL analysis reveals an association between SNPs linked to cardiovascular disease and reduced aortic EC nestin mRNA expression. Our study challenges the dogma that nestin is a marker of proliferation, and provides insight into its regulation and function in EC. Furthermore, our systems-based approach can be applied to investigate body-wide expression profiles of any candidate protein.

KW - Journal Article

U2 - 10.1038/s41598-018-32859-4

DO - 10.1038/s41598-018-32859-4

M3 - SCORING: Journal article

C2 - 30279450

VL - 8

SP - 14668

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

IS - 1

ER -