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A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes

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A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes. / Rauch, Liane; Hennings, Kirsten; Aepfelbacher, Martin.

in: TRAFFIC, Jahrgang 15, Nr. 10, 01.10.2014, S. 1083-98.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Rauch, L, Hennings, K & Aepfelbacher, M 2014, 'A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes', TRAFFIC, Jg. 15, Nr. 10, S. 1083-98. https://doi.org/10.1111/tra.12189

APA

Rauch, L., Hennings, K., & Aepfelbacher, M. (2014). A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes. TRAFFIC, 15(10), 1083-98. https://doi.org/10.1111/tra.12189

Vancouver

Rauch L, Hennings K, Aepfelbacher M. A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes. TRAFFIC. 2014 Okt 1;15(10):1083-98. https://doi.org/10.1111/tra.12189

Bibtex

@article{30edf48d146d48f7b2f8ce16c4461cf9,
title = "A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes",
abstract = "Bacteria that invade human endothelial cells can be efficiently eliminated in phagolysosomes. We investigated the role of vesicle tethering exocyst complex in maturation and function of endothelial cell phagosomes harbouring staphylococci or latex beads. Exocyst complex proteins (Sec5, -8, -10, Exo70) together with recycling endosome marker Rab11 were detected in vesicles that dynamically interacted and seemingly fused with endothelial cell phagosomes. Knockdown of exocyst proteins Sec8 and Exo70 inhibited the accumulation of Rab11-positive vesicles at the phagosomes. Furthermore, knockdown of exocyst proteins and Rab11 greatly reduced acidification of phagosomes and significantly diminished the elimination of invaded staphylococci in endothelial cells. The inhibitory effect of Exo70 knockdown on bacterial elimination could be rescued by constitutively active Rab11-Q70L. Our data suggest that exocyst complex controls the interaction of recycling endocytic vesicles with phagosomes and this process is involved in maturation and functioning of the phagosomes in endothelial cells.",
author = "Liane Rauch and Kirsten Hennings and Martin Aepfelbacher",
note = "{\textcopyright} 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",
year = "2014",
month = oct,
day = "1",
doi = "10.1111/tra.12189",
language = "English",
volume = "15",
pages = "1083--98",
journal = "TRAFFIC",
issn = "1398-9219",
publisher = "Blackwell Munksgaard",
number = "10",

}

RIS

TY - JOUR

T1 - A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes

AU - Rauch, Liane

AU - Hennings, Kirsten

AU - Aepfelbacher, Martin

N1 - © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Bacteria that invade human endothelial cells can be efficiently eliminated in phagolysosomes. We investigated the role of vesicle tethering exocyst complex in maturation and function of endothelial cell phagosomes harbouring staphylococci or latex beads. Exocyst complex proteins (Sec5, -8, -10, Exo70) together with recycling endosome marker Rab11 were detected in vesicles that dynamically interacted and seemingly fused with endothelial cell phagosomes. Knockdown of exocyst proteins Sec8 and Exo70 inhibited the accumulation of Rab11-positive vesicles at the phagosomes. Furthermore, knockdown of exocyst proteins and Rab11 greatly reduced acidification of phagosomes and significantly diminished the elimination of invaded staphylococci in endothelial cells. The inhibitory effect of Exo70 knockdown on bacterial elimination could be rescued by constitutively active Rab11-Q70L. Our data suggest that exocyst complex controls the interaction of recycling endocytic vesicles with phagosomes and this process is involved in maturation and functioning of the phagosomes in endothelial cells.

AB - Bacteria that invade human endothelial cells can be efficiently eliminated in phagolysosomes. We investigated the role of vesicle tethering exocyst complex in maturation and function of endothelial cell phagosomes harbouring staphylococci or latex beads. Exocyst complex proteins (Sec5, -8, -10, Exo70) together with recycling endosome marker Rab11 were detected in vesicles that dynamically interacted and seemingly fused with endothelial cell phagosomes. Knockdown of exocyst proteins Sec8 and Exo70 inhibited the accumulation of Rab11-positive vesicles at the phagosomes. Furthermore, knockdown of exocyst proteins and Rab11 greatly reduced acidification of phagosomes and significantly diminished the elimination of invaded staphylococci in endothelial cells. The inhibitory effect of Exo70 knockdown on bacterial elimination could be rescued by constitutively active Rab11-Q70L. Our data suggest that exocyst complex controls the interaction of recycling endocytic vesicles with phagosomes and this process is involved in maturation and functioning of the phagosomes in endothelial cells.

U2 - 10.1111/tra.12189

DO - 10.1111/tra.12189

M3 - SCORING: Journal article

C2 - 25040264

VL - 15

SP - 1083

EP - 1098

JO - TRAFFIC

JF - TRAFFIC

SN - 1398-9219

IS - 10

ER -