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A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice

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A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice. / Er-Lukowiak, Marco; Duan, Yinghui; Rassendren, Francois; Ulmann, Lauriane; Nicke, Annette; Ufer, Friederike; Friese, Manuel A; Koch-Nolte, Friedrich; Magnus, Tim; Rissiek, Björn.

in: ISCIENCE, Jahrgang 23, Nr. 12, 18.12.2020, S. 101870.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Er-Lukowiak, M, Duan, Y, Rassendren, F, Ulmann, L, Nicke, A, Ufer, F, Friese, MA, Koch-Nolte, F, Magnus, T & Rissiek, B 2020, 'A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice', ISCIENCE, Jg. 23, Nr. 12, S. 101870. https://doi.org/10.1016/j.isci.2020.101870

APA

Er-Lukowiak, M., Duan, Y., Rassendren, F., Ulmann, L., Nicke, A., Ufer, F., Friese, M. A., Koch-Nolte, F., Magnus, T., & Rissiek, B. (2020). A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice. ISCIENCE, 23(12), 101870. https://doi.org/10.1016/j.isci.2020.101870

Vancouver

Er-Lukowiak M, Duan Y, Rassendren F, Ulmann L, Nicke A, Ufer F et al. A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice. ISCIENCE. 2020 Dez 18;23(12):101870. https://doi.org/10.1016/j.isci.2020.101870

Bibtex

@article{25b6043cf0174621bd60e93643ca799d,
title = "A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice",
abstract = "Among laboratory mouse strains many genes are differentially expressed in the same cell population. As consequence, gene targeting in 129-derived embryonic stem cells (ESCs) and backcrossing the modified mice onto the C57BL/6 background can introduce passenger mutations in the close proximity of the targeted gene. Here, we demonstrate that several transgenic mice carry a P2rx7 passenger mutation that affects the function of T cells. By the example of P2rx4tm1Rass we demonstrate that P2X4ko T cells express higher levels of P2X7 and are more sensitive toward the P2X7 activators ATP and NAD+, rendering these cells more vulnerable toward NAD-induced cell death (NICD) compared with wild type (WT). The enhanced NICD sensitivity confounded functional assays e.g. cytokine production and cell migration. Our results need to be considered when working with P2rx4tm1Rass mice or other 129-based transgenic strains that target P2rx7 neighboring genes.",
author = "Marco Er-Lukowiak and Yinghui Duan and Francois Rassendren and Lauriane Ulmann and Annette Nicke and Friederike Ufer and Friese, {Manuel A} and Friedrich Koch-Nolte and Tim Magnus and Bj{\"o}rn Rissiek",
note = "{\textcopyright} 2020 The Author(s).",
year = "2020",
month = dec,
day = "18",
doi = "10.1016/j.isci.2020.101870",
language = "English",
volume = "23",
pages = "101870",
journal = "ISCIENCE",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "12",

}

RIS

TY - JOUR

T1 - A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice

AU - Er-Lukowiak, Marco

AU - Duan, Yinghui

AU - Rassendren, Francois

AU - Ulmann, Lauriane

AU - Nicke, Annette

AU - Ufer, Friederike

AU - Friese, Manuel A

AU - Koch-Nolte, Friedrich

AU - Magnus, Tim

AU - Rissiek, Björn

N1 - © 2020 The Author(s).

PY - 2020/12/18

Y1 - 2020/12/18

N2 - Among laboratory mouse strains many genes are differentially expressed in the same cell population. As consequence, gene targeting in 129-derived embryonic stem cells (ESCs) and backcrossing the modified mice onto the C57BL/6 background can introduce passenger mutations in the close proximity of the targeted gene. Here, we demonstrate that several transgenic mice carry a P2rx7 passenger mutation that affects the function of T cells. By the example of P2rx4tm1Rass we demonstrate that P2X4ko T cells express higher levels of P2X7 and are more sensitive toward the P2X7 activators ATP and NAD+, rendering these cells more vulnerable toward NAD-induced cell death (NICD) compared with wild type (WT). The enhanced NICD sensitivity confounded functional assays e.g. cytokine production and cell migration. Our results need to be considered when working with P2rx4tm1Rass mice or other 129-based transgenic strains that target P2rx7 neighboring genes.

AB - Among laboratory mouse strains many genes are differentially expressed in the same cell population. As consequence, gene targeting in 129-derived embryonic stem cells (ESCs) and backcrossing the modified mice onto the C57BL/6 background can introduce passenger mutations in the close proximity of the targeted gene. Here, we demonstrate that several transgenic mice carry a P2rx7 passenger mutation that affects the function of T cells. By the example of P2rx4tm1Rass we demonstrate that P2X4ko T cells express higher levels of P2X7 and are more sensitive toward the P2X7 activators ATP and NAD+, rendering these cells more vulnerable toward NAD-induced cell death (NICD) compared with wild type (WT). The enhanced NICD sensitivity confounded functional assays e.g. cytokine production and cell migration. Our results need to be considered when working with P2rx4tm1Rass mice or other 129-based transgenic strains that target P2rx7 neighboring genes.

U2 - 10.1016/j.isci.2020.101870

DO - 10.1016/j.isci.2020.101870

M3 - SCORING: Journal article

C2 - 33336163

VL - 23

SP - 101870

JO - ISCIENCE

JF - ISCIENCE

SN - 2589-0042

IS - 12

ER -