PortalPublikationenA novel 'sort-suicide' fusion gene vector for T cell manipul...

A novel 'sort-suicide' fusion gene vector for T cell manipulation

Standard

A novel 'sort-suicide' fusion gene vector for T cell manipulation. / Fehse, B; Kustikova, O S; Li, Z; Wahlers, A; Bohn, W; Beyer, W R; Chalmers, D; Tiberghien, P; Kühlcke, K; Zander, A R; Baum, C.

in: GENE THER, Jahrgang 9, Nr. 23, 12.2002, S. 1633-1638.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › Kurzpublikation › Forschung › Begutachtung

Harvard

Fehse, B, Kustikova, OS, Li, Z, Wahlers, A, Bohn, W, Beyer, WR, Chalmers, D, Tiberghien, P, Kühlcke, K, Zander, AR & Baum, C 2002, 'A novel 'sort-suicide' fusion gene vector for T cell manipulation', GENE THER, Jg. 9, Nr. 23, S. 1633-1638. https://doi.org/10.1038/sj.gt.3301828

APA

Fehse, B., Kustikova, O. S., Li, Z., Wahlers, A., Bohn, W., Beyer, W. R., Chalmers, D., Tiberghien, P., Kühlcke, K., Zander, A. R., & Baum, C. (2002). A novel 'sort-suicide' fusion gene vector for T cell manipulation. GENE THER, 9(23), 1633-1638. https://doi.org/10.1038/sj.gt.3301828

Vancouver

Fehse B, Kustikova OS, Li Z, Wahlers A, Bohn W, Beyer WR et al. A novel 'sort-suicide' fusion gene vector for T cell manipulation. GENE THER. 2002 Dez;9(23):1633-1638. https://doi.org/10.1038/sj.gt.3301828

Bibtex

@article{43b7922736af47ea9f96c3a0f02cab78,
title = "A novel 'sort-suicide' fusion gene vector for T cell manipulation",
abstract = "Retroviral suicide gene vectors have successfully been used in clinical studies to improve the safety of adoptive immunotherapy with allogeneic T lymphocytes in the treatment of malignant and viral diseases. At the same time these studies have revealed several problems that are yet to be resolved including impaired T cell function due to long ex vivo culture. Here we present new retroviral vectors co-expressing truncated CD34, a gene transfer marker which ensures rapid enrichment of transduced cells using commercially available GMP-approved devices, and a splice-corrected variant of Herpes simplex virus thymidine kinase (scHSVtk) which confers high sensitivity to the prodrug ganciclovir. We show that a retroviral hybrid vector, MP71, based on the myeloproliferative sarcoma virus (MPSV) and the murine embryonic stem cell virus (MESV), encoding a tCD34/scHSVtk fusion protein mediates high expression of the 'sort-suicide' selection marker, thereby allowing for highly efficient purification and selective elimination of transduced cells.",
keywords = "Antigens, CD34/genetics, Ganciclovir/pharmacology, Genetic Markers, Genetic Vectors, Humans, Immunotherapy, Adoptive/methods, Jurkat Cells, Lymphocyte Depletion/methods, Microscopy, Confocal, Retroviridae/genetics, T-Lymphocytes/drug effects, Transduction, Genetic",
author = "B Fehse and Kustikova, {O S} and Z Li and A Wahlers and W Bohn and Beyer, {W R} and D Chalmers and P Tiberghien and K K{\"u}hlcke and Zander, {A R} and C Baum",
year = "2002",
month = dec,
doi = "10.1038/sj.gt.3301828",
language = "English",
volume = "9",
pages = "1633--1638",
journal = "GENE THER",
issn = "0969-7128",
publisher = "NATURE PUBLISHING GROUP",
number = "23",

}

RIS

TY - JOUR

T1 - A novel 'sort-suicide' fusion gene vector for T cell manipulation

AU - Fehse, B

AU - Kustikova, O S

AU - Li, Z

AU - Wahlers, A

AU - Bohn, W

AU - Beyer, W R

AU - Chalmers, D

AU - Tiberghien, P

AU - Kühlcke, K

AU - Zander, A R

AU - Baum, C

PY - 2002/12

Y1 - 2002/12

N2 - Retroviral suicide gene vectors have successfully been used in clinical studies to improve the safety of adoptive immunotherapy with allogeneic T lymphocytes in the treatment of malignant and viral diseases. At the same time these studies have revealed several problems that are yet to be resolved including impaired T cell function due to long ex vivo culture. Here we present new retroviral vectors co-expressing truncated CD34, a gene transfer marker which ensures rapid enrichment of transduced cells using commercially available GMP-approved devices, and a splice-corrected variant of Herpes simplex virus thymidine kinase (scHSVtk) which confers high sensitivity to the prodrug ganciclovir. We show that a retroviral hybrid vector, MP71, based on the myeloproliferative sarcoma virus (MPSV) and the murine embryonic stem cell virus (MESV), encoding a tCD34/scHSVtk fusion protein mediates high expression of the 'sort-suicide' selection marker, thereby allowing for highly efficient purification and selective elimination of transduced cells.

AB - Retroviral suicide gene vectors have successfully been used in clinical studies to improve the safety of adoptive immunotherapy with allogeneic T lymphocytes in the treatment of malignant and viral diseases. At the same time these studies have revealed several problems that are yet to be resolved including impaired T cell function due to long ex vivo culture. Here we present new retroviral vectors co-expressing truncated CD34, a gene transfer marker which ensures rapid enrichment of transduced cells using commercially available GMP-approved devices, and a splice-corrected variant of Herpes simplex virus thymidine kinase (scHSVtk) which confers high sensitivity to the prodrug ganciclovir. We show that a retroviral hybrid vector, MP71, based on the myeloproliferative sarcoma virus (MPSV) and the murine embryonic stem cell virus (MESV), encoding a tCD34/scHSVtk fusion protein mediates high expression of the 'sort-suicide' selection marker, thereby allowing for highly efficient purification and selective elimination of transduced cells.

KW - Antigens, CD34/genetics

KW - Ganciclovir/pharmacology

KW - Genetic Markers

KW - Genetic Vectors

KW - Humans

KW - Immunotherapy, Adoptive/methods

KW - Jurkat Cells

KW - Lymphocyte Depletion/methods

KW - Microscopy, Confocal

KW - Retroviridae/genetics

KW - T-Lymphocytes/drug effects

KW - Transduction, Genetic

U2 - 10.1038/sj.gt.3301828

DO - 10.1038/sj.gt.3301828

M3 - Short publication

C2 - 12424616

VL - 9

SP - 1633

EP - 1638

JO - GENE THER

JF - GENE THER

SN - 0969-7128

IS - 23

ER -