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A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA

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A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA. / Papp, Stefanie; Richardt, Ulricke; Fleischer, Bernhard; Osterloh, Anke.

in: Monoclon Antib Immunodiagn Immunother, Jahrgang 32, Nr. 2, 01.04.2013, S. 98-104.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Papp, S, Richardt, U, Fleischer, B & Osterloh, A 2013, 'A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA', Monoclon Antib Immunodiagn Immunother, Jg. 32, Nr. 2, S. 98-104. https://doi.org/10.1089/mab.2012.0080

APA

Papp, S., Richardt, U., Fleischer, B., & Osterloh, A. (2013). A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA. Monoclon Antib Immunodiagn Immunother, 32(2), 98-104. https://doi.org/10.1089/mab.2012.0080

Vancouver

Papp S, Richardt U, Fleischer B, Osterloh A. A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA. Monoclon Antib Immunodiagn Immunother. 2013 Apr 1;32(2):98-104. https://doi.org/10.1089/mab.2012.0080

Bibtex

@article{6517ee47f9974bf6952356a01be83ed6,
title = "A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA",
abstract = "CD83 is expressed on mature dendritic cells (DCs) and many immune cells, including B and T lymphocytes upon activation. CD83 is essential for the development of CD4 single positive T cells and involved in the regulation of peripheral T and B cell responses. Here we generated a new monoclonal antibody against human CD83, which we named ELBE-1. ELBE-1 recognizes a linear epitope and cannot only be applied for Western blot analysis and flow cytometry, but also detects soluble CD83 (sCD83) by ELISA. As with other anti-CD83 antibodies described thus far, ELBE-1 does not influence T cell activation. Nevertheless, it represents a useful new tool for the detection and functional characterization of CD83.",
keywords = "Animals, Antibodies, Monoclonal, Murine-Derived, Antigens, CD, Blotting, Western, COS Cells, Cell Proliferation, Cercopithecus aethiops, Coculture Techniques, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Feeder Cells, Female, Flow Cytometry, Humans, Hybridomas, Immunoglobulins, Leukocytes, Mononuclear, Lymphocyte Activation, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, T-Lymphocytes",
author = "Stefanie Papp and Ulricke Richardt and Bernhard Fleischer and Anke Osterloh",
year = "2013",
month = apr,
day = "1",
doi = "10.1089/mab.2012.0080",
language = "English",
volume = "32",
pages = "98--104",
journal = "Monoclon Antib Immunodiagn Immunother",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA

AU - Papp, Stefanie

AU - Richardt, Ulricke

AU - Fleischer, Bernhard

AU - Osterloh, Anke

PY - 2013/4/1

Y1 - 2013/4/1

N2 - CD83 is expressed on mature dendritic cells (DCs) and many immune cells, including B and T lymphocytes upon activation. CD83 is essential for the development of CD4 single positive T cells and involved in the regulation of peripheral T and B cell responses. Here we generated a new monoclonal antibody against human CD83, which we named ELBE-1. ELBE-1 recognizes a linear epitope and cannot only be applied for Western blot analysis and flow cytometry, but also detects soluble CD83 (sCD83) by ELISA. As with other anti-CD83 antibodies described thus far, ELBE-1 does not influence T cell activation. Nevertheless, it represents a useful new tool for the detection and functional characterization of CD83.

AB - CD83 is expressed on mature dendritic cells (DCs) and many immune cells, including B and T lymphocytes upon activation. CD83 is essential for the development of CD4 single positive T cells and involved in the regulation of peripheral T and B cell responses. Here we generated a new monoclonal antibody against human CD83, which we named ELBE-1. ELBE-1 recognizes a linear epitope and cannot only be applied for Western blot analysis and flow cytometry, but also detects soluble CD83 (sCD83) by ELISA. As with other anti-CD83 antibodies described thus far, ELBE-1 does not influence T cell activation. Nevertheless, it represents a useful new tool for the detection and functional characterization of CD83.

KW - Animals

KW - Antibodies, Monoclonal, Murine-Derived

KW - Antigens, CD

KW - Blotting, Western

KW - COS Cells

KW - Cell Proliferation

KW - Cercopithecus aethiops

KW - Coculture Techniques

KW - Enzyme-Linked Immunosorbent Assay

KW - Epitope Mapping

KW - Feeder Cells

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Hybridomas

KW - Immunoglobulins

KW - Leukocytes, Mononuclear

KW - Lymphocyte Activation

KW - Membrane Glycoproteins

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C3H

KW - T-Lymphocytes

U2 - 10.1089/mab.2012.0080

DO - 10.1089/mab.2012.0080

M3 - SCORING: Journal article

C2 - 23607344

VL - 32

SP - 98

EP - 104

JO - Monoclon Antib Immunodiagn Immunother

JF - Monoclon Antib Immunodiagn Immunother

SN - 2167-9436

IS - 2

ER -