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A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes

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A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes. / Rinschen, Markus M; Gödel, Markus; Grahammer, Florian; Zschiedrich, Stefan; Helmstädter, Martin; Kretz, Oliver; Zarei, Mostafa; Braun, Daniela A; Dittrich, Sebastian; Pahmeyer, Caroline; Schroder, Patricia; Teetzen, Carolin; Gee, HeonYung; Daouk, Ghaleb; Pohl, Martin; Kuhn, Elisa; Schermer, Bernhard; Küttner, Victoria; Boerries, Melanie; Busch, Hauke; Schiffer, Mario; Bergmann, Carsten; Krüger, Marcus; Hildebrandt, Friedhelm; Dengjel, Joern; Benzing, Thomas; Huber, Tobias B.

in: CELL REP, Jahrgang 23, Nr. 8, 22.05.2018, S. 2495-2508.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Rinschen, MM, Gödel, M, Grahammer, F, Zschiedrich, S, Helmstädter, M, Kretz, O, Zarei, M, Braun, DA, Dittrich, S, Pahmeyer, C, Schroder, P, Teetzen, C, Gee, H, Daouk, G, Pohl, M, Kuhn, E, Schermer, B, Küttner, V, Boerries, M, Busch, H, Schiffer, M, Bergmann, C, Krüger, M, Hildebrandt, F, Dengjel, J, Benzing, T & Huber, TB 2018, 'A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes', CELL REP, Jg. 23, Nr. 8, S. 2495-2508. https://doi.org/10.1016/j.celrep.2018.04.059

APA

Rinschen, M. M., Gödel, M., Grahammer, F., Zschiedrich, S., Helmstädter, M., Kretz, O., Zarei, M., Braun, D. A., Dittrich, S., Pahmeyer, C., Schroder, P., Teetzen, C., Gee, H., Daouk, G., Pohl, M., Kuhn, E., Schermer, B., Küttner, V., Boerries, M., ... Huber, T. B. (2018). A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes. CELL REP, 23(8), 2495-2508. https://doi.org/10.1016/j.celrep.2018.04.059

Vancouver

Rinschen MM, Gödel M, Grahammer F, Zschiedrich S, Helmstädter M, Kretz O et al. A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes. CELL REP. 2018 Mai 22;23(8):2495-2508. https://doi.org/10.1016/j.celrep.2018.04.059

Bibtex

@article{ca6a70291a484bf9bd6c6d760aa78e8c,
title = "A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes",
abstract = "Damage to and loss of glomerular podocytes has been identified as the culprit lesion in progressive kidney diseases. Here, we combine mass spectrometry-based proteomics with mRNA sequencing, bioinformatics, and hypothesis-driven studies to provide a comprehensive and quantitative map of mammalian podocytes that identifies unanticipated signaling pathways. Comparison of the in vivo datasets with proteomics data from podocyte cell cultures showed a limited value of available cell culture models. Moreover, in vivo stable isotope labeling by amino acids uncovered surprisingly rapid synthesis of mitochondrial proteins under steady-state conditions that was perturbed under autophagy-deficient, disease-susceptible conditions. Integration of acquired omics dimensions suggested FARP1 as a candidate essential for podocyte function, which could be substantiated by genetic analysis in humans and knockdown experiments in zebrafish. This work exemplifies how the integration of multi-omics datasets can identify a framework of cell-type-specific features relevant for organ health and disease.",
keywords = "Journal Article",
author = "Rinschen, {Markus M} and Markus G{\"o}del and Florian Grahammer and Stefan Zschiedrich and Martin Helmst{\"a}dter and Oliver Kretz and Mostafa Zarei and Braun, {Daniela A} and Sebastian Dittrich and Caroline Pahmeyer and Patricia Schroder and Carolin Teetzen and HeonYung Gee and Ghaleb Daouk and Martin Pohl and Elisa Kuhn and Bernhard Schermer and Victoria K{\"u}ttner and Melanie Boerries and Hauke Busch and Mario Schiffer and Carsten Bergmann and Marcus Kr{\"u}ger and Friedhelm Hildebrandt and Joern Dengjel and Thomas Benzing and Huber, {Tobias B}",
note = "Copyright {\textcopyright} 2018 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = may,
day = "22",
doi = "10.1016/j.celrep.2018.04.059",
language = "English",
volume = "23",
pages = "2495--2508",
journal = "CELL REP",
issn = "2211-1247",
publisher = "Elsevier",
number = "8",

}

RIS

TY - JOUR

T1 - A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes

AU - Rinschen, Markus M

AU - Gödel, Markus

AU - Grahammer, Florian

AU - Zschiedrich, Stefan

AU - Helmstädter, Martin

AU - Kretz, Oliver

AU - Zarei, Mostafa

AU - Braun, Daniela A

AU - Dittrich, Sebastian

AU - Pahmeyer, Caroline

AU - Schroder, Patricia

AU - Teetzen, Carolin

AU - Gee, HeonYung

AU - Daouk, Ghaleb

AU - Pohl, Martin

AU - Kuhn, Elisa

AU - Schermer, Bernhard

AU - Küttner, Victoria

AU - Boerries, Melanie

AU - Busch, Hauke

AU - Schiffer, Mario

AU - Bergmann, Carsten

AU - Krüger, Marcus

AU - Hildebrandt, Friedhelm

AU - Dengjel, Joern

AU - Benzing, Thomas

AU - Huber, Tobias B

N1 - Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2018/5/22

Y1 - 2018/5/22

N2 - Damage to and loss of glomerular podocytes has been identified as the culprit lesion in progressive kidney diseases. Here, we combine mass spectrometry-based proteomics with mRNA sequencing, bioinformatics, and hypothesis-driven studies to provide a comprehensive and quantitative map of mammalian podocytes that identifies unanticipated signaling pathways. Comparison of the in vivo datasets with proteomics data from podocyte cell cultures showed a limited value of available cell culture models. Moreover, in vivo stable isotope labeling by amino acids uncovered surprisingly rapid synthesis of mitochondrial proteins under steady-state conditions that was perturbed under autophagy-deficient, disease-susceptible conditions. Integration of acquired omics dimensions suggested FARP1 as a candidate essential for podocyte function, which could be substantiated by genetic analysis in humans and knockdown experiments in zebrafish. This work exemplifies how the integration of multi-omics datasets can identify a framework of cell-type-specific features relevant for organ health and disease.

AB - Damage to and loss of glomerular podocytes has been identified as the culprit lesion in progressive kidney diseases. Here, we combine mass spectrometry-based proteomics with mRNA sequencing, bioinformatics, and hypothesis-driven studies to provide a comprehensive and quantitative map of mammalian podocytes that identifies unanticipated signaling pathways. Comparison of the in vivo datasets with proteomics data from podocyte cell cultures showed a limited value of available cell culture models. Moreover, in vivo stable isotope labeling by amino acids uncovered surprisingly rapid synthesis of mitochondrial proteins under steady-state conditions that was perturbed under autophagy-deficient, disease-susceptible conditions. Integration of acquired omics dimensions suggested FARP1 as a candidate essential for podocyte function, which could be substantiated by genetic analysis in humans and knockdown experiments in zebrafish. This work exemplifies how the integration of multi-omics datasets can identify a framework of cell-type-specific features relevant for organ health and disease.

KW - Journal Article

U2 - 10.1016/j.celrep.2018.04.059

DO - 10.1016/j.celrep.2018.04.059

M3 - SCORING: Journal article

C2 - 29791858

VL - 23

SP - 2495

EP - 2508

JO - CELL REP

JF - CELL REP

SN - 2211-1247

IS - 8

ER -