A hybrid vector for ligand-directed tumor targeting and molecular imaging

Amin Hajitou; Martin Trepel; Caroline E Lilley; Suren Soghomonyan; Mian M Alauddin; Frank C Marini; Bradley H Restel; Michael G Ozawa; Catherine A Moya; Roberto Rangel; Yan V Sun; Karim Zaoui; Manfred Schmidt; Christof von Kalle; Matthew D Weitzman; Juri G Gelovani; Renata Pasqualini; Wadih Arap

doi:10.1016/j.cell.2006.02.042

A hybrid vector for ligand-directed tumor targeting and molecular imaging

Standard

A hybrid vector for ligand-directed tumor targeting and molecular imaging. / Hajitou, Amin; Trepel, Martin; Lilley, Caroline E; Soghomonyan, Suren; Alauddin, Mian M; Marini, Frank C; Restel, Bradley H; Ozawa, Michael G; Moya, Catherine A; Rangel, Roberto; Sun, Yan V; Zaoui, Karim; Schmidt, Manfred; von Kalle, Christof; Weitzman, Matthew D; Gelovani, Juri G; Pasqualini, Renata; Arap, Wadih.

in: CELL, Jahrgang 125, Nr. 2, 21.04.2006, S. 385-98.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Hajitou, A, Trepel, M, Lilley, CE, Soghomonyan, S, Alauddin, MM, Marini, FC, Restel, BH, Ozawa, MG, Moya, CA, Rangel, R, Sun, YV, Zaoui, K, Schmidt, M, von Kalle, C, Weitzman, MD, Gelovani, JG, Pasqualini, R & Arap, W 2006, 'A hybrid vector for ligand-directed tumor targeting and molecular imaging', CELL, Jg. 125, Nr. 2, S. 385-98. https://doi.org/10.1016/j.cell.2006.02.042

APA

Hajitou, A., Trepel, M., Lilley, C. E., Soghomonyan, S., Alauddin, M. M., Marini, F. C., Restel, B. H., Ozawa, M. G., Moya, C. A., Rangel, R., Sun, Y. V., Zaoui, K., Schmidt, M., von Kalle, C., Weitzman, M. D., Gelovani, J. G., Pasqualini, R., & Arap, W. (2006). A hybrid vector for ligand-directed tumor targeting and molecular imaging. CELL, 125(2), 385-98. https://doi.org/10.1016/j.cell.2006.02.042

Vancouver

Hajitou A, Trepel M, Lilley CE, Soghomonyan S, Alauddin MM, Marini FC et al. A hybrid vector for ligand-directed tumor targeting and molecular imaging. CELL. 2006 Apr 21;125(2):385-98. https://doi.org/10.1016/j.cell.2006.02.042

Bibtex

@article{a77b7f6beffe48d7939c1077e119851a,

title = "A hybrid vector for ligand-directed tumor targeting and molecular imaging",

abstract = "Merging tumor targeting and molecular-genetic imaging into an integrated platform is limited by lack of strategies to enable systemic yet ligand-directed delivery and imaging of specific transgenes. Many eukaryotic viruses serve for transgene delivery but require elimination of native tropism for mammalian cells; in contrast, prokaryotic viruses can be adapted to bind to mammalian receptors but are otherwise poor vehicles. Here we introduce a system containing cis-elements from adeno-associated virus (AAV) and single-stranded bacteriophage. Our AAV/phage (AAVP) prototype targets an integrin. We show that AAVP provides superior tumor transduction over phage and that incorporation of inverted terminal repeats is associated with improved fate of the delivered transgene. Moreover, we show that the temporal dynamics and spatial heterogeneity of gene expression mediated by targeted AAVP can be monitored by positron emission tomography. This new class of targeted hybrid viral particles will enable a wide range of applications in biology and medicine.",

keywords = "Animals, Antiviral Agents, Bacteriophages, Dependovirus, Diagnostic Imaging, Ganciclovir, Gene Transfer Techniques, Genetic Vectors, Integrin alphaV, Ligands, Mice, Mice, Nude, Molecular Biology, Neoplasm Transplantation, Neoplasms, Transduction, Genetic, Transgenes, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.",

author = "Amin Hajitou and Martin Trepel and Lilley, {Caroline E} and Suren Soghomonyan and Alauddin, {Mian M} and Marini, {Frank C} and Restel, {Bradley H} and Ozawa, {Michael G} and Moya, {Catherine A} and Roberto Rangel and Sun, {Yan V} and Karim Zaoui and Manfred Schmidt and {von Kalle}, Christof and Weitzman, {Matthew D} and Gelovani, {Juri G} and Renata Pasqualini and Wadih Arap",

year = "2006",

month = apr,

day = "21",

doi = "10.1016/j.cell.2006.02.042",

language = "English",

volume = "125",

pages = "385--98",

journal = "CELL",

issn = "0092-8674",

publisher = "Cell Press",

number = "2",

}

RIS

TY - JOUR

T1 - A hybrid vector for ligand-directed tumor targeting and molecular imaging

AU - Hajitou, Amin

AU - Trepel, Martin

AU - Lilley, Caroline E

AU - Soghomonyan, Suren

AU - Alauddin, Mian M

AU - Marini, Frank C

AU - Restel, Bradley H

AU - Ozawa, Michael G

AU - Moya, Catherine A

AU - Rangel, Roberto

AU - Sun, Yan V

AU - Zaoui, Karim

AU - Schmidt, Manfred

AU - von Kalle, Christof

AU - Weitzman, Matthew D

AU - Gelovani, Juri G

AU - Pasqualini, Renata

AU - Arap, Wadih

PY - 2006/4/21

Y1 - 2006/4/21

N2 - Merging tumor targeting and molecular-genetic imaging into an integrated platform is limited by lack of strategies to enable systemic yet ligand-directed delivery and imaging of specific transgenes. Many eukaryotic viruses serve for transgene delivery but require elimination of native tropism for mammalian cells; in contrast, prokaryotic viruses can be adapted to bind to mammalian receptors but are otherwise poor vehicles. Here we introduce a system containing cis-elements from adeno-associated virus (AAV) and single-stranded bacteriophage. Our AAV/phage (AAVP) prototype targets an integrin. We show that AAVP provides superior tumor transduction over phage and that incorporation of inverted terminal repeats is associated with improved fate of the delivered transgene. Moreover, we show that the temporal dynamics and spatial heterogeneity of gene expression mediated by targeted AAVP can be monitored by positron emission tomography. This new class of targeted hybrid viral particles will enable a wide range of applications in biology and medicine.

AB - Merging tumor targeting and molecular-genetic imaging into an integrated platform is limited by lack of strategies to enable systemic yet ligand-directed delivery and imaging of specific transgenes. Many eukaryotic viruses serve for transgene delivery but require elimination of native tropism for mammalian cells; in contrast, prokaryotic viruses can be adapted to bind to mammalian receptors but are otherwise poor vehicles. Here we introduce a system containing cis-elements from adeno-associated virus (AAV) and single-stranded bacteriophage. Our AAV/phage (AAVP) prototype targets an integrin. We show that AAVP provides superior tumor transduction over phage and that incorporation of inverted terminal repeats is associated with improved fate of the delivered transgene. Moreover, we show that the temporal dynamics and spatial heterogeneity of gene expression mediated by targeted AAVP can be monitored by positron emission tomography. This new class of targeted hybrid viral particles will enable a wide range of applications in biology and medicine.

KW - Animals

KW - Antiviral Agents

KW - Bacteriophages

KW - Dependovirus

KW - Diagnostic Imaging

KW - Ganciclovir

KW - Gene Transfer Techniques

KW - Genetic Vectors

KW - Integrin alphaV

KW - Ligands

KW - Mice

KW - Mice, Nude

KW - Molecular Biology

KW - Neoplasm Transplantation

KW - Neoplasms

KW - Transduction, Genetic

KW - Transgenes

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

U2 - 10.1016/j.cell.2006.02.042

DO - 10.1016/j.cell.2006.02.042

M3 - SCORING: Journal article

C2 - 16630824

VL - 125

SP - 385

EP - 398

JO - CELL

JF - CELL

SN - 0092-8674

IS - 2

ER -