A CMV-induced adaptive human Vδ1+ γδ T cell clone recognizes HLA-DR
Beteiligte Einrichtungen
Abstract
The innate and adaptive roles of γδ T cells and their clonal γδ T cell receptors (TCRs) in immune responses are still unclear. Recent studies of γδ TCR repertoire dynamics showed massive expansion of individual Vδ1+ γδ T cell clones during viral infection. To judge whether such expansion is random or actually represents TCR-dependent adaptive immune responses, information about their cognate TCR ligands is required. Here, we used CRISPR/Cas9-mediated screening to identify HLA-DRA, RFXAP, RFX5, and CIITA as required for target cell recognition of a CMV-induced Vγ3Vδ1+ TCR, and further characterization revealed a direct interaction of this Vδ1+ TCR with the MHC II complex HLA-DR. Since MHC II is strongly upregulated by interferon-γ, these results suggest an inflammation-induced MHC-dependent immune response of γδ T cells.
Bibliografische Daten
Originalsprache | Englisch |
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Aufsatznummer | e20212525 |
ISSN | 0022-1007 |
DOIs | |
Status | Veröffentlicht - 05.09.2022 |
Anmerkungen des Dekanats
© 2022 Deseke et al.
PubMed | 35852466 |
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