A Chicken Embryo Model for the Study of Umbilical and Supraumbilical Body Wall Malformations

TY - JOUR

T1 - A Chicken Embryo Model for the Study of Umbilical and Supraumbilical Body Wall Malformations

AU - Ridderbusch, Ina

AU - Bergholz, Robert

AU - Fattouh, Miriam

AU - Eschenburg, Georg

AU - Roth, Beate

AU - Appl, Birgit

AU - Maenner, Joerg

AU - Reinshagen, Konrad

AU - Kluth, Dietrich

N1 - Georg Thieme Verlag KG Stuttgart · New York.

PY - 2015

Y1 - 2015

N2 - Background/Purpose The embryology of ventral body wall malformations is only partially understood, although their incidence is relatively common. As only few experimental data exist on the development of those defects, the aim of our study was to compare the teratogenic effect of trypan blue (TB) and suramin (SA) in their capability to induce umbilical and supraumbilical abdominal wall malformations in a chicken egg model. Materials and Methods A total of 255 fertilized chicken eggs were incubated at 38°C and 75% relative humidity. Embryos were treated in ovo on incubation day 2.5 (Hamburger/Hamilton (HH) stage 13). The eggshell was windowed, and solutions of TB or SA were injected into the coelomic cavity at the region of the umbilicus. The window was closed and the embryos reincubated until examination on day 8 (HH 34). Results A total of 60 embryos survived in each group. The largest number of embryos presented with defects in the umbilical and supraumbilical region (25% in the SA group and 40% in the TB group). A combination of both defects (thoracoabdominoschisis) was seen in 20% of the TB and 8.3% of the SA groups, respectively. Associated anomalies found in both groups were head and eye defects, abnormal pelvic configurations, leg deformities, and mild forms of cloacal exstrophies. Conclusions TB and SA have both a high potential to induce umbilical and supraumbilical ventral body wall malformations in chicken embryos. This novel animal model might help to establish a more profound understanding of the developmental steps in ventral body wall formation and the embryology for its malformations.

AB - Background/Purpose The embryology of ventral body wall malformations is only partially understood, although their incidence is relatively common. As only few experimental data exist on the development of those defects, the aim of our study was to compare the teratogenic effect of trypan blue (TB) and suramin (SA) in their capability to induce umbilical and supraumbilical abdominal wall malformations in a chicken egg model. Materials and Methods A total of 255 fertilized chicken eggs were incubated at 38°C and 75% relative humidity. Embryos were treated in ovo on incubation day 2.5 (Hamburger/Hamilton (HH) stage 13). The eggshell was windowed, and solutions of TB or SA were injected into the coelomic cavity at the region of the umbilicus. The window was closed and the embryos reincubated until examination on day 8 (HH 34). Results A total of 60 embryos survived in each group. The largest number of embryos presented with defects in the umbilical and supraumbilical region (25% in the SA group and 40% in the TB group). A combination of both defects (thoracoabdominoschisis) was seen in 20% of the TB and 8.3% of the SA groups, respectively. Associated anomalies found in both groups were head and eye defects, abnormal pelvic configurations, leg deformities, and mild forms of cloacal exstrophies. Conclusions TB and SA have both a high potential to induce umbilical and supraumbilical ventral body wall malformations in chicken embryos. This novel animal model might help to establish a more profound understanding of the developmental steps in ventral body wall formation and the embryology for its malformations.

U2 - 10.1055/s-0034-1371718

DO - 10.1055/s-0034-1371718

M3 - SCORING: Journal article

C2 - 24744060

VL - 25

SP - 257

EP - 261

JO - EUR J PEDIATR SURG

JF - EUR J PEDIATR SURG

SN - 0939-7248

IS - 3

ER -