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A biomechanical, micro-computertomographic and histological analysis of the influence of diclofenac and prednisolone on fracture healing in vivo

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A biomechanical, micro-computertomographic and histological analysis of the influence of diclofenac and prednisolone on fracture healing in vivo. / Bissinger, Oliver; Kreutzer, Kilian; Götz, Carolin; Hapfelmeier, Alexander; Pautke, Christoph; Vogt, Stephan; Wexel, Gabriele; Wolff, Klaus-Dietrich; Tischer, Thomas; Prodinger, Peter Michael.

in: BMC MUSCULOSKEL DIS, Jahrgang 17, Nr. 1, 2016, S. 383.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bissinger, O, Kreutzer, K, Götz, C, Hapfelmeier, A, Pautke, C, Vogt, S, Wexel, G, Wolff, K-D, Tischer, T & Prodinger, PM 2016, 'A biomechanical, micro-computertomographic and histological analysis of the influence of diclofenac and prednisolone on fracture healing in vivo', BMC MUSCULOSKEL DIS, Jg. 17, Nr. 1, S. 383. https://doi.org/10.1186/s12891-016-1241-2

APA

Bissinger, O., Kreutzer, K., Götz, C., Hapfelmeier, A., Pautke, C., Vogt, S., Wexel, G., Wolff, K-D., Tischer, T., & Prodinger, P. M. (2016). A biomechanical, micro-computertomographic and histological analysis of the influence of diclofenac and prednisolone on fracture healing in vivo. BMC MUSCULOSKEL DIS, 17(1), 383. https://doi.org/10.1186/s12891-016-1241-2

Vancouver

Bissinger O, Kreutzer K, Götz C, Hapfelmeier A, Pautke C, Vogt S et al. A biomechanical, micro-computertomographic and histological analysis of the influence of diclofenac and prednisolone on fracture healing in vivo. BMC MUSCULOSKEL DIS. 2016;17(1):383. https://doi.org/10.1186/s12891-016-1241-2

Bibtex

@article{b47e0d4e7e584e0a8412ffef55e26a23,
title = "A biomechanical, micro-computertomographic and histological analysis of the influence of diclofenac and prednisolone on fracture healing in vivo",
abstract = "BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAIDs) have long been suspected of negatively affecting fracture healing, although numerous disputes still exist and little data are available regarding diclofenac. Glucocorticoids interfere in this process over a similar and even broader mechanism of action. As many previously conducted studies evaluated either morphological changes or biomechanical properties of treated bones, the conjunction of both structural measures is completely missing. Therefore, it was our aim to evaluate the effects of diclofenac and prednisolone on the fracture callus biomechanically, morphologically and by 3-dimensional (3D) microstructural analysis.METHODS: Femura of diclofenac-, prednisolone- or placebo-treated rats were pinned and a closed transverse fracture was generated. After 21 days, biomechanics, micro-CT (μCT) and histology were examined.RESULTS: The diclofenac group showed significantly impaired fracture healing compared with the control group by biomechanics and μCT (e.g. stiffness: 57.31 ± 31.11 N/mm vs. 122.44 ± 81.16 N/mm, p = 0.030; callus volume: 47.05 ± 15.67 mm3 vs. 67.19 ± 14.90 mm3, p = 0.037, trabecular thickness: 0.0937 mm ± 0.003 vs. 0.0983 mm ± 0.003, p = 0.023), as confirmed by histology. Biomechanics of the prednisolone group showed obviously lower absolute values than the control group. These alterations were confirmed in conjunction with μCT and histology.CONCLUSIONS: The inhibiting effects of both substances were not only mediated by absolute parameters (e.g. breaking load, BV), but we have shown, for the first time, that additional changes occurred in the microstructural bony network. Especially in patients at risk for delayed bone healing (arteriosclerosis, diabetes mellitus, smoking), the administration of these drugs should be weighed carefully.",
keywords = "Journal Article",
author = "Oliver Bissinger and Kilian Kreutzer and Carolin G{\"o}tz and Alexander Hapfelmeier and Christoph Pautke and Stephan Vogt and Gabriele Wexel and Klaus-Dietrich Wolff and Thomas Tischer and Prodinger, {Peter Michael}",
year = "2016",
doi = "10.1186/s12891-016-1241-2",
language = "English",
volume = "17",
pages = "383",
journal = "BMC MUSCULOSKEL DIS",
issn = "1471-2474",
publisher = "BioMed Central Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - A biomechanical, micro-computertomographic and histological analysis of the influence of diclofenac and prednisolone on fracture healing in vivo

AU - Bissinger, Oliver

AU - Kreutzer, Kilian

AU - Götz, Carolin

AU - Hapfelmeier, Alexander

AU - Pautke, Christoph

AU - Vogt, Stephan

AU - Wexel, Gabriele

AU - Wolff, Klaus-Dietrich

AU - Tischer, Thomas

AU - Prodinger, Peter Michael

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAIDs) have long been suspected of negatively affecting fracture healing, although numerous disputes still exist and little data are available regarding diclofenac. Glucocorticoids interfere in this process over a similar and even broader mechanism of action. As many previously conducted studies evaluated either morphological changes or biomechanical properties of treated bones, the conjunction of both structural measures is completely missing. Therefore, it was our aim to evaluate the effects of diclofenac and prednisolone on the fracture callus biomechanically, morphologically and by 3-dimensional (3D) microstructural analysis.METHODS: Femura of diclofenac-, prednisolone- or placebo-treated rats were pinned and a closed transverse fracture was generated. After 21 days, biomechanics, micro-CT (μCT) and histology were examined.RESULTS: The diclofenac group showed significantly impaired fracture healing compared with the control group by biomechanics and μCT (e.g. stiffness: 57.31 ± 31.11 N/mm vs. 122.44 ± 81.16 N/mm, p = 0.030; callus volume: 47.05 ± 15.67 mm3 vs. 67.19 ± 14.90 mm3, p = 0.037, trabecular thickness: 0.0937 mm ± 0.003 vs. 0.0983 mm ± 0.003, p = 0.023), as confirmed by histology. Biomechanics of the prednisolone group showed obviously lower absolute values than the control group. These alterations were confirmed in conjunction with μCT and histology.CONCLUSIONS: The inhibiting effects of both substances were not only mediated by absolute parameters (e.g. breaking load, BV), but we have shown, for the first time, that additional changes occurred in the microstructural bony network. Especially in patients at risk for delayed bone healing (arteriosclerosis, diabetes mellitus, smoking), the administration of these drugs should be weighed carefully.

AB - BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAIDs) have long been suspected of negatively affecting fracture healing, although numerous disputes still exist and little data are available regarding diclofenac. Glucocorticoids interfere in this process over a similar and even broader mechanism of action. As many previously conducted studies evaluated either morphological changes or biomechanical properties of treated bones, the conjunction of both structural measures is completely missing. Therefore, it was our aim to evaluate the effects of diclofenac and prednisolone on the fracture callus biomechanically, morphologically and by 3-dimensional (3D) microstructural analysis.METHODS: Femura of diclofenac-, prednisolone- or placebo-treated rats were pinned and a closed transverse fracture was generated. After 21 days, biomechanics, micro-CT (μCT) and histology were examined.RESULTS: The diclofenac group showed significantly impaired fracture healing compared with the control group by biomechanics and μCT (e.g. stiffness: 57.31 ± 31.11 N/mm vs. 122.44 ± 81.16 N/mm, p = 0.030; callus volume: 47.05 ± 15.67 mm3 vs. 67.19 ± 14.90 mm3, p = 0.037, trabecular thickness: 0.0937 mm ± 0.003 vs. 0.0983 mm ± 0.003, p = 0.023), as confirmed by histology. Biomechanics of the prednisolone group showed obviously lower absolute values than the control group. These alterations were confirmed in conjunction with μCT and histology.CONCLUSIONS: The inhibiting effects of both substances were not only mediated by absolute parameters (e.g. breaking load, BV), but we have shown, for the first time, that additional changes occurred in the microstructural bony network. Especially in patients at risk for delayed bone healing (arteriosclerosis, diabetes mellitus, smoking), the administration of these drugs should be weighed carefully.

KW - Journal Article

U2 - 10.1186/s12891-016-1241-2

DO - 10.1186/s12891-016-1241-2

M3 - SCORING: Journal article

C2 - 27596101

VL - 17

SP - 383

JO - BMC MUSCULOSKEL DIS

JF - BMC MUSCULOSKEL DIS

SN - 1471-2474

IS - 1

ER -