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3D skin models for 3R research

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3D skin models for 3R research : The potential of 3D reconstructed skin models to study skin barrier function. / Niehues, Hanna; Bouwstra, Joke A; El Ghalbzouri, Abdoelwaheb; Brandner, Johanna M; Zeeuwen, Patrick L J M; van den Bogaard, Ellen H.

in: EXP DERMATOL, Jahrgang 27, Nr. 5, 05.2018, S. 501-511.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Review › Forschung

Harvard

Niehues, H, Bouwstra, JA, El Ghalbzouri, A, Brandner, JM, Zeeuwen, PLJM & van den Bogaard, EH 2018, '3D skin models for 3R research: The potential of 3D reconstructed skin models to study skin barrier function', EXP DERMATOL, Jg. 27, Nr. 5, S. 501-511. https://doi.org/10.1111/exd.13531

APA

Niehues, H., Bouwstra, J. A., El Ghalbzouri, A., Brandner, J. M., Zeeuwen, P. L. J. M., & van den Bogaard, E. H. (2018). 3D skin models for 3R research: The potential of 3D reconstructed skin models to study skin barrier function. EXP DERMATOL, 27(5), 501-511. https://doi.org/10.1111/exd.13531

Vancouver

Niehues H, Bouwstra JA, El Ghalbzouri A, Brandner JM, Zeeuwen PLJM, van den Bogaard EH. 3D skin models for 3R research: The potential of 3D reconstructed skin models to study skin barrier function. EXP DERMATOL. 2018 Mai;27(5):501-511. https://doi.org/10.1111/exd.13531

Bibtex

@article{823592d688504d23b0cf066bb698ee0b,
title = "3D skin models for 3R research: The potential of 3D reconstructed skin models to study skin barrier function",
abstract = "The skin barrier is an important shield regulating the outside-in as well as inside-out penetration of water, nutrients, ions and environmental stimuli. We can distinguish four different barrier compartments: the physical, chemical, immunological and microbial skin barrier. Well-functioning of those is needed to protect our body from the environment. To better understand the function and the contribution of barrier dysfunction in skin diseases, 3D skin or epidermal models are a valuable tool for in vitro studies. In this review, we summarize the development and application of different skin models in skin barrier research. During the last years, enormous effort was made on optimizing these models to better mimic the in vivo composition of the skin, by fine-tuning cell culture media, culture conditions and including additional cells and tissue components. Thereby, in vitro barrier formation and function has been improved significantly. Moreover, in this review we point towards changes and chances for in vitro 3D skin models to be used for skin barrier research in the nearby future.",
keywords = "Animal Use Alternatives, Humans, In Vitro Techniques, Microbiota, Models, Biological, Permeability, Skin/metabolism, Tight Junctions",
author = "Hanna Niehues and Bouwstra, {Joke A} and {El Ghalbzouri}, Abdoelwaheb and Brandner, {Johanna M} and Zeeuwen, {Patrick L J M} and {van den Bogaard}, {Ellen H}",
note = "{\textcopyright} 2018 The Authors. Experimental Dermatology Published by John Wiley & Sons Ltd.",
year = "2018",
month = may,
doi = "10.1111/exd.13531",
language = "English",
volume = "27",
pages = "501--511",
journal = "EXP DERMATOL",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - 3D skin models for 3R research

T2 - The potential of 3D reconstructed skin models to study skin barrier function

AU - Niehues, Hanna

AU - Bouwstra, Joke A

AU - El Ghalbzouri, Abdoelwaheb

AU - Brandner, Johanna M

AU - Zeeuwen, Patrick L J M

AU - van den Bogaard, Ellen H

N1 - © 2018 The Authors. Experimental Dermatology Published by John Wiley & Sons Ltd.

PY - 2018/5

Y1 - 2018/5

N2 - The skin barrier is an important shield regulating the outside-in as well as inside-out penetration of water, nutrients, ions and environmental stimuli. We can distinguish four different barrier compartments: the physical, chemical, immunological and microbial skin barrier. Well-functioning of those is needed to protect our body from the environment. To better understand the function and the contribution of barrier dysfunction in skin diseases, 3D skin or epidermal models are a valuable tool for in vitro studies. In this review, we summarize the development and application of different skin models in skin barrier research. During the last years, enormous effort was made on optimizing these models to better mimic the in vivo composition of the skin, by fine-tuning cell culture media, culture conditions and including additional cells and tissue components. Thereby, in vitro barrier formation and function has been improved significantly. Moreover, in this review we point towards changes and chances for in vitro 3D skin models to be used for skin barrier research in the nearby future.

AB - The skin barrier is an important shield regulating the outside-in as well as inside-out penetration of water, nutrients, ions and environmental stimuli. We can distinguish four different barrier compartments: the physical, chemical, immunological and microbial skin barrier. Well-functioning of those is needed to protect our body from the environment. To better understand the function and the contribution of barrier dysfunction in skin diseases, 3D skin or epidermal models are a valuable tool for in vitro studies. In this review, we summarize the development and application of different skin models in skin barrier research. During the last years, enormous effort was made on optimizing these models to better mimic the in vivo composition of the skin, by fine-tuning cell culture media, culture conditions and including additional cells and tissue components. Thereby, in vitro barrier formation and function has been improved significantly. Moreover, in this review we point towards changes and chances for in vitro 3D skin models to be used for skin barrier research in the nearby future.

KW - Animal Use Alternatives

KW - Humans

KW - In Vitro Techniques

KW - Microbiota

KW - Models, Biological

KW - Permeability

KW - Skin/metabolism

KW - Tight Junctions

U2 - 10.1111/exd.13531

DO - 10.1111/exd.13531

M3 - SCORING: Review article

C2 - 29518287

VL - 27

SP - 501

EP - 511

JO - EXP DERMATOL

JF - EXP DERMATOL

SN - 0906-6705

IS - 5

ER -