Research ExplorerPublicationsThe HARE chip for efficient time-resolved serial synchrotron...

The HARE chip for efficient time-resolved serial synchrotron crystallography

Standard

The HARE chip for efficient time-resolved serial synchrotron crystallography. / Mehrabi, Pedram; Müller-Werkmeister, Henrike M; Leimkohl, Jan Philipp; Schikora, Hendrik; Ninkovic, Jelena; Krivokuca, Silvia; Andriček, Ladislav; Epp, Sascha W; Sherrell, Darren; Owen, Robin L; Pearson, Arwen R; Tellkamp, Friedjof; Schulz, Eike C; Miller, R J Dwayne.

In: J SYNCHROTRON RADIAT, Vol. 27, No. Pt 2, 01.03.2020, p. 360-370.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mehrabi, P, Müller-Werkmeister, HM, Leimkohl, JP, Schikora, H, Ninkovic, J, Krivokuca, S, Andriček, L, Epp, SW, Sherrell, D, Owen, RL, Pearson, AR, Tellkamp, F, Schulz, EC & Miller, RJD 2020, 'The HARE chip for efficient time-resolved serial synchrotron crystallography', J SYNCHROTRON RADIAT, vol. 27, no. Pt 2, pp. 360-370. https://doi.org/10.1107/S1600577520000685

APA

Mehrabi, P., Müller-Werkmeister, H. M., Leimkohl, J. P., Schikora, H., Ninkovic, J., Krivokuca, S., Andriček, L., Epp, S. W., Sherrell, D., Owen, R. L., Pearson, A. R., Tellkamp, F., Schulz, E. C., & Miller, R. J. D. (2020). The HARE chip for efficient time-resolved serial synchrotron crystallography. J SYNCHROTRON RADIAT, 27(Pt 2), 360-370. https://doi.org/10.1107/S1600577520000685

Vancouver

Mehrabi P, Müller-Werkmeister HM, Leimkohl JP, Schikora H, Ninkovic J, Krivokuca S et al. The HARE chip for efficient time-resolved serial synchrotron crystallography. J SYNCHROTRON RADIAT. 2020 Mar 1;27(Pt 2):360-370. https://doi.org/10.1107/S1600577520000685

Bibtex

@article{c80b1fae6fe7420dabf3a42cbfa74467,
title = "The HARE chip for efficient time-resolved serial synchrotron crystallography",
abstract = "Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.",
author = "Pedram Mehrabi and M{\"u}ller-Werkmeister, {Henrike M} and Leimkohl, {Jan Philipp} and Hendrik Schikora and Jelena Ninkovic and Silvia Krivokuca and Ladislav Andri{\v c}ek and Epp, {Sascha W} and Darren Sherrell and Owen, {Robin L} and Pearson, {Arwen R} and Friedjof Tellkamp and Schulz, {Eike C} and Miller, {R J Dwayne}",
note = "open access.",
year = "2020",
month = mar,
day = "1",
doi = "10.1107/S1600577520000685",
language = "English",
volume = "27",
pages = "360--370",
journal = "J SYNCHROTRON RADIAT",
issn = "0909-0495",
publisher = "International Union of Crystallography",
number = "Pt 2",

}

RIS

TY - JOUR

T1 - The HARE chip for efficient time-resolved serial synchrotron crystallography

AU - Mehrabi, Pedram

AU - Müller-Werkmeister, Henrike M

AU - Leimkohl, Jan Philipp

AU - Schikora, Hendrik

AU - Ninkovic, Jelena

AU - Krivokuca, Silvia

AU - Andriček, Ladislav

AU - Epp, Sascha W

AU - Sherrell, Darren

AU - Owen, Robin L

AU - Pearson, Arwen R

AU - Tellkamp, Friedjof

AU - Schulz, Eike C

AU - Miller, R J Dwayne

N1 - open access.

PY - 2020/3/1

Y1 - 2020/3/1

N2 - Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.

AB - Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.

U2 - 10.1107/S1600577520000685

DO - 10.1107/S1600577520000685

M3 - SCORING: Journal article

C2 - 32153274

VL - 27

SP - 360

EP - 370

JO - J SYNCHROTRON RADIAT

JF - J SYNCHROTRON RADIAT

SN - 0909-0495

IS - Pt 2

ER -