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The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons.

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The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons. / Cui, Xuezhi; Weng, Ying-Qi; Frappé, Isabelle; Burgess, Alison; Cruz, Girão Da; Schachner, Melitta; Schachner, Melitta; Aubert, Isabelle.

In: BRAIN BEHAV, Vol. 1, No. 2, 2, 2011, p. 73-86.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Cui, X, Weng, Y-Q, Frappé, I, Burgess, A, Cruz, GD, Schachner, M, Schachner, M & Aubert, I 2011, 'The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons.', BRAIN BEHAV, vol. 1, no. 2, 2, pp. 73-86. https://doi.org/10.1002/brb3.15

APA

Cui, X., Weng, Y-Q., Frappé, I., Burgess, A., Cruz, G. D., Schachner, M., Schachner, M., & Aubert, I. (2011). The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons. BRAIN BEHAV, 1(2), 73-86. [2]. https://doi.org/10.1002/brb3.15

Vancouver

Cui X, Weng Y-Q, Frappé I, Burgess A, Cruz GD, Schachner M et al. The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons. BRAIN BEHAV. 2011;1(2):73-86. 2. https://doi.org/10.1002/brb3.15

Bibtex

@article{802a0dd6977c491eab7dcba3143430e7,
title = "The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons.",
abstract = "Mutations in the L1 gene cause severe brain malformations and mental retardation. We investigated the potential roles of L1 in the regulation of choline acetyltransferase (ChAT) and in the development of septal cholinergic neurons, which are known to project to the hippocampus and play key roles in cognitive functions. Using stereological approaches, we detected significantly fewer ChAT-positive cholinergic neurons in the medial septum and vertical limb of the diagonal band of Broca (MS/VDB) of 2-week-old L1-deficient mice compared to wild-type littermates (1644 ± 137 vs. 2051 ± 165, P = 0.038). ChAT protein levels in the septum were 53% lower in 2-week-old L1-deficient mice compared to wild-type littermates. ChAT activity in the septum was significantly reduced in L1-deficient mice compared to wild-type littermates at 1 (34%) and 2 (40%) weeks of age. In vitro, increasing doses of L1-Fc induced ChAT activity in septal neurons with a significant linear trend (*P = 0.0065). At 4 weeks of age in the septum and at all time points investigated in the caudate-putamen (CPu), the number of ChAT-positive neurons and the levels of ChAT activity were not statistically different between L1-deficient mice and wild-type littermates. The total number of cells positive for the neuronal nuclear antigen (NeuN) in the MS/VDB and CPu was not statistically different in L1-deficient mice compared to wild-type littermates, and comparable expression of the cell cycle marker Ki67 was observed. Our results indicate that L1 is required for the timely maturation of septal cholinergic neurons and that L1 promotes the expression and activity of ChAT in septal neurons.",
author = "Xuezhi Cui and Ying-Qi Weng and Isabelle Frapp{\'e} and Alison Burgess and Cruz, {Gir{\~a}o Da} and Melitta Schachner and Melitta Schachner and Isabelle Aubert",
year = "2011",
doi = "10.1002/brb3.15",
language = "English",
volume = "1",
pages = "73--86",
journal = "BRAIN BEHAV",
issn = "2162-3279",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - The cell adhesion molecule L1 regulates the expression of choline acetyltransferase and the development of septal cholinergic neurons.

AU - Cui, Xuezhi

AU - Weng, Ying-Qi

AU - Frappé, Isabelle

AU - Burgess, Alison

AU - Cruz, Girão Da

AU - Schachner, Melitta

AU - Schachner, Melitta

AU - Aubert, Isabelle

PY - 2011

Y1 - 2011

N2 - Mutations in the L1 gene cause severe brain malformations and mental retardation. We investigated the potential roles of L1 in the regulation of choline acetyltransferase (ChAT) and in the development of septal cholinergic neurons, which are known to project to the hippocampus and play key roles in cognitive functions. Using stereological approaches, we detected significantly fewer ChAT-positive cholinergic neurons in the medial septum and vertical limb of the diagonal band of Broca (MS/VDB) of 2-week-old L1-deficient mice compared to wild-type littermates (1644 ± 137 vs. 2051 ± 165, P = 0.038). ChAT protein levels in the septum were 53% lower in 2-week-old L1-deficient mice compared to wild-type littermates. ChAT activity in the septum was significantly reduced in L1-deficient mice compared to wild-type littermates at 1 (34%) and 2 (40%) weeks of age. In vitro, increasing doses of L1-Fc induced ChAT activity in septal neurons with a significant linear trend (*P = 0.0065). At 4 weeks of age in the septum and at all time points investigated in the caudate-putamen (CPu), the number of ChAT-positive neurons and the levels of ChAT activity were not statistically different between L1-deficient mice and wild-type littermates. The total number of cells positive for the neuronal nuclear antigen (NeuN) in the MS/VDB and CPu was not statistically different in L1-deficient mice compared to wild-type littermates, and comparable expression of the cell cycle marker Ki67 was observed. Our results indicate that L1 is required for the timely maturation of septal cholinergic neurons and that L1 promotes the expression and activity of ChAT in septal neurons.

AB - Mutations in the L1 gene cause severe brain malformations and mental retardation. We investigated the potential roles of L1 in the regulation of choline acetyltransferase (ChAT) and in the development of septal cholinergic neurons, which are known to project to the hippocampus and play key roles in cognitive functions. Using stereological approaches, we detected significantly fewer ChAT-positive cholinergic neurons in the medial septum and vertical limb of the diagonal band of Broca (MS/VDB) of 2-week-old L1-deficient mice compared to wild-type littermates (1644 ± 137 vs. 2051 ± 165, P = 0.038). ChAT protein levels in the septum were 53% lower in 2-week-old L1-deficient mice compared to wild-type littermates. ChAT activity in the septum was significantly reduced in L1-deficient mice compared to wild-type littermates at 1 (34%) and 2 (40%) weeks of age. In vitro, increasing doses of L1-Fc induced ChAT activity in septal neurons with a significant linear trend (*P = 0.0065). At 4 weeks of age in the septum and at all time points investigated in the caudate-putamen (CPu), the number of ChAT-positive neurons and the levels of ChAT activity were not statistically different between L1-deficient mice and wild-type littermates. The total number of cells positive for the neuronal nuclear antigen (NeuN) in the MS/VDB and CPu was not statistically different in L1-deficient mice compared to wild-type littermates, and comparable expression of the cell cycle marker Ki67 was observed. Our results indicate that L1 is required for the timely maturation of septal cholinergic neurons and that L1 promotes the expression and activity of ChAT in septal neurons.

U2 - 10.1002/brb3.15

DO - 10.1002/brb3.15

M3 - SCORING: Journal article

VL - 1

SP - 73

EP - 86

JO - BRAIN BEHAV

JF - BRAIN BEHAV

SN - 2162-3279

IS - 2

M1 - 2

ER -