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Specific CD4+ T Cell Responses to Ancestral SARS-CoV-2 in Children Increase With Age and Show Cross-Reactivity to Beta Variant

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Specific CD4+ T Cell Responses to Ancestral SARS-CoV-2 in Children Increase With Age and Show Cross-Reactivity to Beta Variant. / Paul, Kevin; Sibbertsen, Freya; Weiskopf, Daniela; Lütgehetmann, Marc; Barroso, Madalena; Danecka, Marta K.; Glau, Laura; Hecher, Laura; Hermann, Katharina; Kohl, Aloisa; Oh, Jun; Wiesch, Julian Schulze zur; Sette, Alessandro; Tolosa, Eva; Vettorazzi, Eik; Woidy, Mathias; Zapf, Antonia; Zazara, Dimitra E.; Mir, Thomas S.; Muntau, Ania C.; Gersting, Søren W.; Dunay, Gábor A.

In: FRONT IMMUNOL, Vol. 13, 867577, 15.07.2022, p. 867577.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Paul, K, Sibbertsen, F, Weiskopf, D, Lütgehetmann, M, Barroso, M, Danecka, MK, Glau, L, Hecher, L, Hermann, K, Kohl, A, Oh, J, Wiesch, JSZ, Sette, A, Tolosa, E, Vettorazzi, E, Woidy, M, Zapf, A, Zazara, DE, Mir, TS, Muntau, AC, Gersting, SW & Dunay, GA 2022, 'Specific CD4+ T Cell Responses to Ancestral SARS-CoV-2 in Children Increase With Age and Show Cross-Reactivity to Beta Variant', FRONT IMMUNOL, vol. 13, 867577, pp. 867577. https://doi.org/10.3389/fimmu.2022.867577

APA

Paul, K., Sibbertsen, F., Weiskopf, D., Lütgehetmann, M., Barroso, M., Danecka, M. K., Glau, L., Hecher, L., Hermann, K., Kohl, A., Oh, J., Wiesch, J. S. Z., Sette, A., Tolosa, E., Vettorazzi, E., Woidy, M., Zapf, A., Zazara, D. E., Mir, T. S., ... Dunay, G. A. (2022). Specific CD4+ T Cell Responses to Ancestral SARS-CoV-2 in Children Increase With Age and Show Cross-Reactivity to Beta Variant. FRONT IMMUNOL, 13, 867577. [867577]. https://doi.org/10.3389/fimmu.2022.867577

Vancouver

Paul K, Sibbertsen F, Weiskopf D, Lütgehetmann M, Barroso M, Danecka MK et al. Specific CD4+ T Cell Responses to Ancestral SARS-CoV-2 in Children Increase With Age and Show Cross-Reactivity to Beta Variant. FRONT IMMUNOL. 2022 Jul 15;13:867577. 867577. https://doi.org/10.3389/fimmu.2022.867577

Bibtex

@article{9e897f1a03e54551b62e61b4d6bac746,
title = "Specific CD4+ T Cell Responses to Ancestral SARS-CoV-2 in Children Increase With Age and Show Cross-Reactivity to Beta Variant",
abstract = "SARS-CoV-2 is still a major burden for global health despite effective vaccines. With the reduction of social distancing measures, infection rates are increasing in children, while data on the pediatric immune response to SARS-CoV-2 infection is still lacking. Although the typical disease course in children has been mild, emerging variants may present new challenges in this age group. Peripheral blood mononuclear cells (PBMC) from 51 convalescent children, 24 seronegative siblings from early 2020, and 51 unexposed controls were stimulated with SARS-CoV-2-derived peptide MegaPools from the ancestral and beta variants. Flow cytometric determination of activation-induced markers and secreted cytokines were used to quantify the CD4+ T cell response. The average time after infection was over 80 days. CD4+ T cell responses were detected in 61% of convalescent children and were markedly reduced in preschool children. Cross-reactive T cells for the SARS-CoV-2 beta variant were identified in 45% of cases after infection with an ancestral SARS-CoV-2 variant. The CD4+ T cell response was accompanied most predominantly by IFN-γ and Granzyme B secretion. An antiviral CD4+ T cell response was present in children after ancestral SARS-CoV-2 infection, which was reduced in the youngest age group. We detected significant cross-reactivity of CD4+ T cell responses to the more recently evolved immune-escaping beta variant. Our findings have epidemiologic relevance for children regarding novel viral variants of concern and vaccination efforts.",
keywords = "CD4-Positive T-Lymphocytes, COVID-19, Child, Child, Preschool, Humans, Leukocytes, Mononuclear, SARS-CoV-2",
author = "Kevin Paul and Freya Sibbertsen and Daniela Weiskopf and Marc L{\"u}tgehetmann and Madalena Barroso and Danecka, {Marta K.} and Laura Glau and Laura Hecher and Katharina Hermann and Aloisa Kohl and Jun Oh and Wiesch, {Julian Schulze zur} and Alessandro Sette and Eva Tolosa and Eik Vettorazzi and Mathias Woidy and Antonia Zapf and Zazara, {Dimitra E.} and Mir, {Thomas S.} and Muntau, {Ania C.} and Gersting, {S{\o}ren W.} and Dunay, {G{\'a}bor A.}",
note = "Copyright {\textcopyright} 2022 Paul, Sibbertsen, Weiskopf, L{\"u}tgehetmann, Barroso, Danecka, Glau, Hecher, Hermann, Kohl, Oh, Schulze zur Wiesch, Sette, Tolosa, Vettorazzi, Woidy, Zapf, Zazara, Mir, Muntau, Gersting and Dunay.",
year = "2022",
month = jul,
day = "15",
doi = "10.3389/fimmu.2022.867577",
language = "English",
volume = "13",
pages = "867577",
journal = "FRONT IMMUNOL",
issn = "1664-3224",
publisher = "Lausanne : Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Specific CD4+ T Cell Responses to Ancestral SARS-CoV-2 in Children Increase With Age and Show Cross-Reactivity to Beta Variant

AU - Paul, Kevin

AU - Sibbertsen, Freya

AU - Weiskopf, Daniela

AU - Lütgehetmann, Marc

AU - Barroso, Madalena

AU - Danecka, Marta K.

AU - Glau, Laura

AU - Hecher, Laura

AU - Hermann, Katharina

AU - Kohl, Aloisa

AU - Oh, Jun

AU - Wiesch, Julian Schulze zur

AU - Sette, Alessandro

AU - Tolosa, Eva

AU - Vettorazzi, Eik

AU - Woidy, Mathias

AU - Zapf, Antonia

AU - Zazara, Dimitra E.

AU - Mir, Thomas S.

AU - Muntau, Ania C.

AU - Gersting, Søren W.

AU - Dunay, Gábor A.

N1 - Copyright © 2022 Paul, Sibbertsen, Weiskopf, Lütgehetmann, Barroso, Danecka, Glau, Hecher, Hermann, Kohl, Oh, Schulze zur Wiesch, Sette, Tolosa, Vettorazzi, Woidy, Zapf, Zazara, Mir, Muntau, Gersting and Dunay.

PY - 2022/7/15

Y1 - 2022/7/15

N2 - SARS-CoV-2 is still a major burden for global health despite effective vaccines. With the reduction of social distancing measures, infection rates are increasing in children, while data on the pediatric immune response to SARS-CoV-2 infection is still lacking. Although the typical disease course in children has been mild, emerging variants may present new challenges in this age group. Peripheral blood mononuclear cells (PBMC) from 51 convalescent children, 24 seronegative siblings from early 2020, and 51 unexposed controls were stimulated with SARS-CoV-2-derived peptide MegaPools from the ancestral and beta variants. Flow cytometric determination of activation-induced markers and secreted cytokines were used to quantify the CD4+ T cell response. The average time after infection was over 80 days. CD4+ T cell responses were detected in 61% of convalescent children and were markedly reduced in preschool children. Cross-reactive T cells for the SARS-CoV-2 beta variant were identified in 45% of cases after infection with an ancestral SARS-CoV-2 variant. The CD4+ T cell response was accompanied most predominantly by IFN-γ and Granzyme B secretion. An antiviral CD4+ T cell response was present in children after ancestral SARS-CoV-2 infection, which was reduced in the youngest age group. We detected significant cross-reactivity of CD4+ T cell responses to the more recently evolved immune-escaping beta variant. Our findings have epidemiologic relevance for children regarding novel viral variants of concern and vaccination efforts.

AB - SARS-CoV-2 is still a major burden for global health despite effective vaccines. With the reduction of social distancing measures, infection rates are increasing in children, while data on the pediatric immune response to SARS-CoV-2 infection is still lacking. Although the typical disease course in children has been mild, emerging variants may present new challenges in this age group. Peripheral blood mononuclear cells (PBMC) from 51 convalescent children, 24 seronegative siblings from early 2020, and 51 unexposed controls were stimulated with SARS-CoV-2-derived peptide MegaPools from the ancestral and beta variants. Flow cytometric determination of activation-induced markers and secreted cytokines were used to quantify the CD4+ T cell response. The average time after infection was over 80 days. CD4+ T cell responses were detected in 61% of convalescent children and were markedly reduced in preschool children. Cross-reactive T cells for the SARS-CoV-2 beta variant were identified in 45% of cases after infection with an ancestral SARS-CoV-2 variant. The CD4+ T cell response was accompanied most predominantly by IFN-γ and Granzyme B secretion. An antiviral CD4+ T cell response was present in children after ancestral SARS-CoV-2 infection, which was reduced in the youngest age group. We detected significant cross-reactivity of CD4+ T cell responses to the more recently evolved immune-escaping beta variant. Our findings have epidemiologic relevance for children regarding novel viral variants of concern and vaccination efforts.

KW - CD4-Positive T-Lymphocytes

KW - COVID-19

KW - Child

KW - Child, Preschool

KW - Humans

KW - Leukocytes, Mononuclear

KW - SARS-CoV-2

U2 - 10.3389/fimmu.2022.867577

DO - 10.3389/fimmu.2022.867577

M3 - SCORING: Journal article

C2 - 35911689

VL - 13

SP - 867577

JO - FRONT IMMUNOL

JF - FRONT IMMUNOL

SN - 1664-3224

M1 - 867577

ER -