Research ExplorerPublicationsRole for Escherichia coli YidD in membrane protein insertion

Role for Escherichia coli YidD in membrane protein insertion

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Role for Escherichia coli YidD in membrane protein insertion. / Yu, Zhong; Lavèn, Mariëlle; Klepsch, Mirjam; de Gier, Jan-Willem; Bitter, Wilbert; van Ulsen, Peter; Luirink, Joen.

In: Journal of bacteriology, Vol. 193, No. 19, 10.2011, p. 5242-51.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Yu, Z, Lavèn, M, Klepsch, M, de Gier, J-W, Bitter, W, van Ulsen, P & Luirink, J 2011, 'Role for Escherichia coli YidD in membrane protein insertion', Journal of bacteriology, vol. 193, no. 19, pp. 5242-51. https://doi.org/10.1128/JB.05429-11

APA

Yu, Z., Lavèn, M., Klepsch, M., de Gier, J-W., Bitter, W., van Ulsen, P., & Luirink, J. (2011). Role for Escherichia coli YidD in membrane protein insertion. Journal of bacteriology, 193(19), 5242-51. https://doi.org/10.1128/JB.05429-11

Vancouver

Yu Z, Lavèn M, Klepsch M, de Gier J-W, Bitter W, van Ulsen P et al. Role for Escherichia coli YidD in membrane protein insertion. Journal of bacteriology. 2011 Oct;193(19):5242-51. https://doi.org/10.1128/JB.05429-11

Bibtex

@article{fa615083bb2c44c68f38a60b5a7f7e45,
title = "Role for Escherichia coli YidD in membrane protein insertion",
abstract = "YidC has an essential but poorly defined function in membrane protein insertion and folding in bacteria. The yidC gene is located in a gene cluster that is highly conserved in Gram-negative bacteria, the gene order being rpmH, rnpA, yidD, yidC, and trmE. Here, we show that Escherichia coli yidD, which overlaps with rnpA and is only 2 bp upstream of yidC, is expressed and localizes to the inner membrane, probably through an amphipathic helix. Inactivation of yidD had no discernible effect on cell growth and viability. However, compared to control cells, ΔyidD cells were affected in the insertion and processing of three YidC-dependent inner membrane proteins. Furthermore, in vitro cross-linking showed that YidD is in proximity of a nascent inner membrane protein during its localization in the Sec-YidC translocon, suggesting that YidD might be involved in the insertion process.",
keywords = "Amino Acid Sequence, Cell Membrane, Escherichia coli, Escherichia coli Proteins, Membrane Proteins, Membrane Transport Proteins, Microscopy, Fluorescence, Molecular Sequence Data, Protein Transport, Sequence Homology, Amino Acid, Journal Article",
author = "Zhong Yu and Mari{\"e}lle Lav{\`e}n and Mirjam Klepsch and {de Gier}, Jan-Willem and Wilbert Bitter and {van Ulsen}, Peter and Joen Luirink",
year = "2011",
month = oct,
doi = "10.1128/JB.05429-11",
language = "English",
volume = "193",
pages = "5242--51",
journal = "J BACTERIOL",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "19",

}

RIS

TY - JOUR

T1 - Role for Escherichia coli YidD in membrane protein insertion

AU - Yu, Zhong

AU - Lavèn, Mariëlle

AU - Klepsch, Mirjam

AU - de Gier, Jan-Willem

AU - Bitter, Wilbert

AU - van Ulsen, Peter

AU - Luirink, Joen

PY - 2011/10

Y1 - 2011/10

N2 - YidC has an essential but poorly defined function in membrane protein insertion and folding in bacteria. The yidC gene is located in a gene cluster that is highly conserved in Gram-negative bacteria, the gene order being rpmH, rnpA, yidD, yidC, and trmE. Here, we show that Escherichia coli yidD, which overlaps with rnpA and is only 2 bp upstream of yidC, is expressed and localizes to the inner membrane, probably through an amphipathic helix. Inactivation of yidD had no discernible effect on cell growth and viability. However, compared to control cells, ΔyidD cells were affected in the insertion and processing of three YidC-dependent inner membrane proteins. Furthermore, in vitro cross-linking showed that YidD is in proximity of a nascent inner membrane protein during its localization in the Sec-YidC translocon, suggesting that YidD might be involved in the insertion process.

AB - YidC has an essential but poorly defined function in membrane protein insertion and folding in bacteria. The yidC gene is located in a gene cluster that is highly conserved in Gram-negative bacteria, the gene order being rpmH, rnpA, yidD, yidC, and trmE. Here, we show that Escherichia coli yidD, which overlaps with rnpA and is only 2 bp upstream of yidC, is expressed and localizes to the inner membrane, probably through an amphipathic helix. Inactivation of yidD had no discernible effect on cell growth and viability. However, compared to control cells, ΔyidD cells were affected in the insertion and processing of three YidC-dependent inner membrane proteins. Furthermore, in vitro cross-linking showed that YidD is in proximity of a nascent inner membrane protein during its localization in the Sec-YidC translocon, suggesting that YidD might be involved in the insertion process.

KW - Amino Acid Sequence

KW - Cell Membrane

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Membrane Proteins

KW - Membrane Transport Proteins

KW - Microscopy, Fluorescence

KW - Molecular Sequence Data

KW - Protein Transport

KW - Sequence Homology, Amino Acid

KW - Journal Article

U2 - 10.1128/JB.05429-11

DO - 10.1128/JB.05429-11

M3 - SCORING: Journal article

C2 - 21803992

VL - 193

SP - 5242

EP - 5251

JO - J BACTERIOL

JF - J BACTERIOL

SN - 0021-9193

IS - 19

ER -