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Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa.

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Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. / Stierl, Manuela; Stumpf, Patrick; Udwari, Daniel; Gueta, Ronnie; Hagedorn, Rolf; Losi, Aba; Gärtner, Wolfgang; Petereit, Linda; Efetova, Marina; Schwarzel, Martin; Oertner, Thomas G.; Nagel, Georg; Hegemann, Peter.

In: J BIOL CHEM, Vol. 286, No. 2, 2, 2011, p. 1181-1188.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Stierl, M, Stumpf, P, Udwari, D, Gueta, R, Hagedorn, R, Losi, A, Gärtner, W, Petereit, L, Efetova, M, Schwarzel, M, Oertner, TG, Nagel, G & Hegemann, P 2011, 'Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa.', J BIOL CHEM, vol. 286, no. 2, 2, pp. 1181-1188. <http://www.ncbi.nlm.nih.gov/pubmed/21030594?dopt=Citation>

APA

Stierl, M., Stumpf, P., Udwari, D., Gueta, R., Hagedorn, R., Losi, A., Gärtner, W., Petereit, L., Efetova, M., Schwarzel, M., Oertner, T. G., Nagel, G., & Hegemann, P. (2011). Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J BIOL CHEM, 286(2), 1181-1188. [2]. http://www.ncbi.nlm.nih.gov/pubmed/21030594?dopt=Citation

Vancouver

Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A et al. Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J BIOL CHEM. 2011;286(2):1181-1188. 2.

Bibtex

@article{b63282eef3924a48b0d6d0df567a715c,
title = "Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa.",
abstract = "The recent success of channelrhodopsin in optogenetics has also caused increasing interest in enzymes that are directly activated by light. We have identified in the genome of the bacterium Beggiatoa a DNA sequence encoding an adenylyl cyclase directly linked to a BLUF (blue light receptor using FAD) type light sensor domain. In Escherichia coli and Xenopus oocytes, this photoactivated adenylyl cyclase (bPAC) showed cyclase activity that is low in darkness but increased 300-fold in the light. This enzymatic activity decays thermally within 20 s in parallel with the red-shifted BLUF photointermediate. bPAC is well expressed in pyramidal neurons and, in combination with cyclic nucleotide gated channels, causes efficient light-induced depolarization. In the Drosophila central nervous system, bPAC mediates light-dependent cAMP increase and behavioral changes in freely moving animals. bPAC seems a perfect optogenetic tool for light modulation of cAMP in neuronal cells and tissues and for studying cAMP-dependent processes in live animals.",
keywords = "Animals, Humans, Rats, Amino Acid Sequence, Molecular Sequence Data, Rats, Wistar, Xenopus, Hippocampus/cytology, *Light, *Adenylate Cyclase/genetics/metabolism/radiation effects, Animals, Genetically Modified, Beggiatoa/*enzymology/*genetics, Cyclic AMP/*metabolism, Cystic Fibrosis Transmembrane Conductance Regulator/genetics, Drosophila/enzymology/genetics, Enzyme Activation/radiation effects, Escherichia coli/enzymology/genetics, Excitatory Postsynaptic Potentials/physiology, Neurons/cytology/physiology, Oocytes/physiology, Photochemistry, Animals, Humans, Rats, Amino Acid Sequence, Molecular Sequence Data, Rats, Wistar, Xenopus, Hippocampus/cytology, *Light, *Adenylate Cyclase/genetics/metabolism/radiation effects, Animals, Genetically Modified, Beggiatoa/*enzymology/*genetics, Cyclic AMP/*metabolism, Cystic Fibrosis Transmembrane Conductance Regulator/genetics, Drosophila/enzymology/genetics, Enzyme Activation/radiation effects, Escherichia coli/enzymology/genetics, Excitatory Postsynaptic Potentials/physiology, Neurons/cytology/physiology, Oocytes/physiology, Photochemistry",
author = "Manuela Stierl and Patrick Stumpf and Daniel Udwari and Ronnie Gueta and Rolf Hagedorn and Aba Losi and Wolfgang G{\"a}rtner and Linda Petereit and Marina Efetova and Martin Schwarzel and Oertner, {Thomas G.} and Georg Nagel and Peter Hegemann",
year = "2011",
language = "English",
volume = "286",
pages = "1181--1188",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa.

AU - Stierl, Manuela

AU - Stumpf, Patrick

AU - Udwari, Daniel

AU - Gueta, Ronnie

AU - Hagedorn, Rolf

AU - Losi, Aba

AU - Gärtner, Wolfgang

AU - Petereit, Linda

AU - Efetova, Marina

AU - Schwarzel, Martin

AU - Oertner, Thomas G.

AU - Nagel, Georg

AU - Hegemann, Peter

PY - 2011

Y1 - 2011

N2 - The recent success of channelrhodopsin in optogenetics has also caused increasing interest in enzymes that are directly activated by light. We have identified in the genome of the bacterium Beggiatoa a DNA sequence encoding an adenylyl cyclase directly linked to a BLUF (blue light receptor using FAD) type light sensor domain. In Escherichia coli and Xenopus oocytes, this photoactivated adenylyl cyclase (bPAC) showed cyclase activity that is low in darkness but increased 300-fold in the light. This enzymatic activity decays thermally within 20 s in parallel with the red-shifted BLUF photointermediate. bPAC is well expressed in pyramidal neurons and, in combination with cyclic nucleotide gated channels, causes efficient light-induced depolarization. In the Drosophila central nervous system, bPAC mediates light-dependent cAMP increase and behavioral changes in freely moving animals. bPAC seems a perfect optogenetic tool for light modulation of cAMP in neuronal cells and tissues and for studying cAMP-dependent processes in live animals.

AB - The recent success of channelrhodopsin in optogenetics has also caused increasing interest in enzymes that are directly activated by light. We have identified in the genome of the bacterium Beggiatoa a DNA sequence encoding an adenylyl cyclase directly linked to a BLUF (blue light receptor using FAD) type light sensor domain. In Escherichia coli and Xenopus oocytes, this photoactivated adenylyl cyclase (bPAC) showed cyclase activity that is low in darkness but increased 300-fold in the light. This enzymatic activity decays thermally within 20 s in parallel with the red-shifted BLUF photointermediate. bPAC is well expressed in pyramidal neurons and, in combination with cyclic nucleotide gated channels, causes efficient light-induced depolarization. In the Drosophila central nervous system, bPAC mediates light-dependent cAMP increase and behavioral changes in freely moving animals. bPAC seems a perfect optogenetic tool for light modulation of cAMP in neuronal cells and tissues and for studying cAMP-dependent processes in live animals.

KW - Animals

KW - Humans

KW - Rats

KW - Amino Acid Sequence

KW - Molecular Sequence Data

KW - Rats, Wistar

KW - Xenopus

KW - Hippocampus/cytology

KW - Light

KW - Adenylate Cyclase/genetics/metabolism/radiation effects

KW - Animals, Genetically Modified

KW - Beggiatoa/enzymology/genetics

KW - Cyclic AMP/metabolism

KW - Cystic Fibrosis Transmembrane Conductance Regulator/genetics

KW - Drosophila/enzymology/genetics

KW - Enzyme Activation/radiation effects

KW - Escherichia coli/enzymology/genetics

KW - Excitatory Postsynaptic Potentials/physiology

KW - Neurons/cytology/physiology

KW - Oocytes/physiology

KW - Photochemistry

KW - Animals

KW - Humans

KW - Rats

KW - Amino Acid Sequence

KW - Molecular Sequence Data

KW - Rats, Wistar

KW - Xenopus

KW - Hippocampus/cytology

KW - Light

KW - Adenylate Cyclase/genetics/metabolism/radiation effects

KW - Animals, Genetically Modified

KW - Beggiatoa/enzymology/genetics

KW - Cyclic AMP/metabolism

KW - Cystic Fibrosis Transmembrane Conductance Regulator/genetics

KW - Drosophila/enzymology/genetics

KW - Enzyme Activation/radiation effects

KW - Escherichia coli/enzymology/genetics

KW - Excitatory Postsynaptic Potentials/physiology

KW - Neurons/cytology/physiology

KW - Oocytes/physiology

KW - Photochemistry

M3 - SCORING: Journal article

VL - 286

SP - 1181

EP - 1188

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 2

M1 - 2

ER -