Laboratory intercomparison of the cytokinesis-block micronucleus assay
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Laboratory intercomparison of the cytokinesis-block micronucleus assay. / Romm, H; Barnard, S; Boulay-Greene, H; De Amicis, A; De Sanctis, S; Franco, M; Herodin, F; Jones, A; Kulka, U; Lista, F; Martigne, P; Moquet, J; Oestreicher, U; Rothkamm, K; Thierens, H; Valente, M; Vandersickel, V; Vral, A; Braselmann, H; Meineke, V; Abend, M; Beinke, C.
In: RADIAT RES, Vol. 180, No. 2, 08.2013, p. 120-8.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Laboratory intercomparison of the cytokinesis-block micronucleus assay
AU - Romm, H
AU - Barnard, S
AU - Boulay-Greene, H
AU - De Amicis, A
AU - De Sanctis, S
AU - Franco, M
AU - Herodin, F
AU - Jones, A
AU - Kulka, U
AU - Lista, F
AU - Martigne, P
AU - Moquet, J
AU - Oestreicher, U
AU - Rothkamm, K
AU - Thierens, H
AU - Valente, M
AU - Vandersickel, V
AU - Vral, A
AU - Braselmann, H
AU - Meineke, V
AU - Abend, M
AU - Beinke, C
PY - 2013/8
Y1 - 2013/8
N2 - The focus of the study is an intercomparison of laboratories' dose-assessment performances using the cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as blind samples (0.1-6.4 Gy) were sent to the participants. The CBMN assay was performed according to protocols individually established and varying among participating laboratories. The time taken to report dose estimates was documented for each laboratory. Additional information concerning laboratory organization/characteristics as well as assay performance was collected. The mean absolute difference (MAD) was calculated and radiation doses were merged into four triage categories reflecting clinical aspects to calculate accuracy, sensitivity and specificity. The earliest report time was 4 days after sample arrival. The CBMN dose estimates were reported with high accuracy (MAD values of 0.20-0.50 Gy at doses below 6.4 Gy for both manual and automated scoring procedures), but showed a limitation of the assay at the dose point of 6.4 Gy, which resulted in a clear dose underestimation in all cases. The MAD values (without 6.4 Gy) differed significantly (P = 0.03) between manual (0.25 Gy, SEM = 0.06, n = 4) or automated scoring procedures (0.37 Gy, SEM = 0.08, n = 5), but lowest MAD were equal (0.2 Gy) for both scoring procedures. Likewise, both scoring procedures led to the same allocation of dose estimates to triage categories of clinical significance (about 83% accuracy and up to 100% specificity).
AB - The focus of the study is an intercomparison of laboratories' dose-assessment performances using the cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as blind samples (0.1-6.4 Gy) were sent to the participants. The CBMN assay was performed according to protocols individually established and varying among participating laboratories. The time taken to report dose estimates was documented for each laboratory. Additional information concerning laboratory organization/characteristics as well as assay performance was collected. The mean absolute difference (MAD) was calculated and radiation doses were merged into four triage categories reflecting clinical aspects to calculate accuracy, sensitivity and specificity. The earliest report time was 4 days after sample arrival. The CBMN dose estimates were reported with high accuracy (MAD values of 0.20-0.50 Gy at doses below 6.4 Gy for both manual and automated scoring procedures), but showed a limitation of the assay at the dose point of 6.4 Gy, which resulted in a clear dose underestimation in all cases. The MAD values (without 6.4 Gy) differed significantly (P = 0.03) between manual (0.25 Gy, SEM = 0.06, n = 4) or automated scoring procedures (0.37 Gy, SEM = 0.08, n = 5), but lowest MAD were equal (0.2 Gy) for both scoring procedures. Likewise, both scoring procedures led to the same allocation of dose estimates to triage categories of clinical significance (about 83% accuracy and up to 100% specificity).
KW - Adult
KW - Automation
KW - Biological Assay/methods
KW - Cells, Cultured/radiation effects
KW - Cytokinesis/radiation effects
KW - Dose-Response Relationship, Radiation
KW - Humans
KW - Laboratory Proficiency Testing
KW - Leukocytes/radiation effects
KW - Male
KW - Micronucleus Tests/methods
KW - Radiation Injuries/diagnosis
KW - Radioactive Hazard Release
KW - Radiometry/methods
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Single-Blind Method
KW - Time Factors
KW - Triage/methods
U2 - 10.1667/RR3234.1
DO - 10.1667/RR3234.1
M3 - SCORING: Journal article
C2 - 23862731
VL - 180
SP - 120
EP - 128
IS - 2
ER -