Research ExplorerPublicationsIdentification and characterisation of novel Mss4-binding Ra...

Identification and characterisation of novel Mss4-binding Rab GTPases

Standard

Identification and characterisation of novel Mss4-binding Rab GTPases. / Wixler, Viktor; Wixler, Ludmilla; Altenfeld, Anika; Ludwig, Stephan; Goody, Roger S; Itzen, Aymelt.

In: BIOL CHEM, Vol. 392, No. 3, 03.2011, p. 239-48.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wixler, V, Wixler, L, Altenfeld, A, Ludwig, S, Goody, RS & Itzen, A 2011, 'Identification and characterisation of novel Mss4-binding Rab GTPases', BIOL CHEM, vol. 392, no. 3, pp. 239-48. https://doi.org/10.1515/BC.2011.022

APA

Wixler, V., Wixler, L., Altenfeld, A., Ludwig, S., Goody, R. S., & Itzen, A. (2011). Identification and characterisation of novel Mss4-binding Rab GTPases. BIOL CHEM, 392(3), 239-48. https://doi.org/10.1515/BC.2011.022

Vancouver

Wixler V, Wixler L, Altenfeld A, Ludwig S, Goody RS, Itzen A. Identification and characterisation of novel Mss4-binding Rab GTPases. BIOL CHEM. 2011 Mar;392(3):239-48. https://doi.org/10.1515/BC.2011.022

Bibtex

@article{ff1752c936ef4878bd568f9a532636c0,
title = "Identification and characterisation of novel Mss4-binding Rab GTPases",
abstract = "The Mss4 (mammalian suppressor of yeast Sec4) is an evolutionarily highly conserved protein and is expressed in all mammalian tissues. Although its precise biological function is still elusive, it has been shown to associate with a subset of secretory Rab proteins (Rab1b, Rab3a, Rab8a, Rab10) and to possess a rather low guanine nucleotide exchange factor (GEF) activity towards them in vitro (Rab1, Rab3a and Rab8a). By screening a human placenta cDNA library with Mss4 as bait, we identified several Rab GTPases (Rab12, Rab13 and Rab18) as novel Mss4-binding Rab proteins. Only exocytic but no endocytic Rab GTPases were found in our search. The binding of Mss4 to Rab proteins was confirmed by direct yeast two-hybrid interaction, by co-immunoprecipitation from lysates of mammalian cells, by immunofluorescence colocalisation as well as by direct in vitro binding studies. Analysis of Mss4 catalytic activity towards different Rab substrates confirmed that it is a somewhat inefficient GEF. These data, together with our mutational analysis of Mss4-Rab binding capacity, support the already proposed idea that Mss4 functions rather as a chaperone for exocytic Rab GTPases than as a GEF.",
keywords = "Amino Acid Sequence, Animals, Fluorescent Antibody Technique, Guanine Nucleotide Exchange Factors, HEK293 Cells, Humans, Mice, Molecular Chaperones, Molecular Sequence Data, Mutation, NIH 3T3 Cells, Protein Binding, rab GTP-Binding Proteins, Journal Article, Research Support, Non-U.S. Gov't",
author = "Viktor Wixler and Ludmilla Wixler and Anika Altenfeld and Stephan Ludwig and Goody, {Roger S} and Aymelt Itzen",
year = "2011",
month = mar,
doi = "10.1515/BC.2011.022",
language = "English",
volume = "392",
pages = "239--48",
journal = "BIOL CHEM",
issn = "1431-6730",
publisher = "Walter de Gruyter GmbH & Co. KG",
number = "3",

}

RIS

TY - JOUR

T1 - Identification and characterisation of novel Mss4-binding Rab GTPases

AU - Wixler, Viktor

AU - Wixler, Ludmilla

AU - Altenfeld, Anika

AU - Ludwig, Stephan

AU - Goody, Roger S

AU - Itzen, Aymelt

PY - 2011/3

Y1 - 2011/3

N2 - The Mss4 (mammalian suppressor of yeast Sec4) is an evolutionarily highly conserved protein and is expressed in all mammalian tissues. Although its precise biological function is still elusive, it has been shown to associate with a subset of secretory Rab proteins (Rab1b, Rab3a, Rab8a, Rab10) and to possess a rather low guanine nucleotide exchange factor (GEF) activity towards them in vitro (Rab1, Rab3a and Rab8a). By screening a human placenta cDNA library with Mss4 as bait, we identified several Rab GTPases (Rab12, Rab13 and Rab18) as novel Mss4-binding Rab proteins. Only exocytic but no endocytic Rab GTPases were found in our search. The binding of Mss4 to Rab proteins was confirmed by direct yeast two-hybrid interaction, by co-immunoprecipitation from lysates of mammalian cells, by immunofluorescence colocalisation as well as by direct in vitro binding studies. Analysis of Mss4 catalytic activity towards different Rab substrates confirmed that it is a somewhat inefficient GEF. These data, together with our mutational analysis of Mss4-Rab binding capacity, support the already proposed idea that Mss4 functions rather as a chaperone for exocytic Rab GTPases than as a GEF.

AB - The Mss4 (mammalian suppressor of yeast Sec4) is an evolutionarily highly conserved protein and is expressed in all mammalian tissues. Although its precise biological function is still elusive, it has been shown to associate with a subset of secretory Rab proteins (Rab1b, Rab3a, Rab8a, Rab10) and to possess a rather low guanine nucleotide exchange factor (GEF) activity towards them in vitro (Rab1, Rab3a and Rab8a). By screening a human placenta cDNA library with Mss4 as bait, we identified several Rab GTPases (Rab12, Rab13 and Rab18) as novel Mss4-binding Rab proteins. Only exocytic but no endocytic Rab GTPases were found in our search. The binding of Mss4 to Rab proteins was confirmed by direct yeast two-hybrid interaction, by co-immunoprecipitation from lysates of mammalian cells, by immunofluorescence colocalisation as well as by direct in vitro binding studies. Analysis of Mss4 catalytic activity towards different Rab substrates confirmed that it is a somewhat inefficient GEF. These data, together with our mutational analysis of Mss4-Rab binding capacity, support the already proposed idea that Mss4 functions rather as a chaperone for exocytic Rab GTPases than as a GEF.

KW - Amino Acid Sequence

KW - Animals

KW - Fluorescent Antibody Technique

KW - Guanine Nucleotide Exchange Factors

KW - HEK293 Cells

KW - Humans

KW - Mice

KW - Molecular Chaperones

KW - Molecular Sequence Data

KW - Mutation

KW - NIH 3T3 Cells

KW - Protein Binding

KW - rab GTP-Binding Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1515/BC.2011.022

DO - 10.1515/BC.2011.022

M3 - SCORING: Journal article

C2 - 21194374

VL - 392

SP - 239

EP - 248

JO - BIOL CHEM

JF - BIOL CHEM

SN - 1431-6730

IS - 3

ER -