Human and Murine T-Helper Cell Recovery from Organs and Tissues

Abstract

Isolation of cells from organs and tissues represents a challenge for many researchers. Cell yield, purity, and cell death are common problems associated with it, which greatly affect experimental results in terms of reproducibility and biological observations. Here, we describe state-of-the-art protocols of how to isolate CD4+ T cells from both human and murine organs and tissues, reducing at minimum cell death, while increasing at the same time cell yield and purity.

Bibliographical data

Original languageEnglish
ISSN1064-3745
DOIs
Publication statusPublished - 2021
PubMed 33928539