From high-throughput cell culture screening to mouse model: identification of new inhibitor classes against prion disease

  • Markus Geissen
  • Fabienne Leidel
  • Martin Eiden
  • Thomas Hirschberger
  • Christine Fast
  • Uwe Bertsch
  • Paul Tavan
  • Armin Giese
  • Hans Kretzschmar
  • Hermann M Schatzl
  • Martin H Groschup

Related Research units

Abstract

Transmissible spongiform encephalopathies (TSE) or prion diseases belong to a category of fatal and so far untreatable neurodegenerative conditions. All prion diseases are characterized by both degeneration in the central nervous system (CNS) in humans and animals and the deposition and accumulation of Proteinase K-resistant prion protein (PrP(res)). Until now, no pharmaceutical product has been available to cure these diseases or to alleviate their associated symptoms. Here, a cell-culture screening system is described that allows for the large-scale analysis of the PrP(res) inhibitory potential of a library of compounds and the identification of structural motifs leading potent compounds able to cause PrP(res) clearance at the cellular level. Based on different scrapie-infected cell lines, 10,000 substances were tested, out of which 530 potential inhibitors were identified. After re-screening and validation using a series of dilutions, 14 compounds were identified as the most effective. These 14 compounds were then used for therapeutic studies in a mouse bioassay to test and verify their in vivo potency. Two compounds exhibited therapeutic potential in the mouse model by significantly extending the survival time of intracerebrally infected mice, when treated 90 days after infection with scrapie.

Bibliographical data

Original languageEnglish
ISSN1860-7179
DOIs
Publication statusPublished - 04.10.2011

Comment Deanary

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PubMed 21755599