Effect of local and intravenous lidocaine on ongoing activity in injured afferent nerve fibers.
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Effect of local and intravenous lidocaine on ongoing activity in injured afferent nerve fibers. / Kirillova, Irina; Teliban, Alina; Gorodetskaya, Natalia; Grossmann, Lydia; Bartsch, Fabian; Rausch, Vanessa; Struck, Marek; Tode, Jan; Baron, Ralf; Jänig, Wilfrid.
In: PAIN, Vol. 152, No. 7, 7, 2011, p. 1562-1571.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of local and intravenous lidocaine on ongoing activity in injured afferent nerve fibers.
AU - Kirillova, Irina
AU - Teliban, Alina
AU - Gorodetskaya, Natalia
AU - Grossmann, Lydia
AU - Bartsch, Fabian
AU - Rausch, Vanessa
AU - Struck, Marek
AU - Tode, Jan
AU - Baron, Ralf
AU - Jänig, Wilfrid
PY - 2011
Y1 - 2011
N2 - Lidocaine applied systemically or locally attenuates neuropathic pain in patients. Here we tested the hypothesis that ectopic activity in injured afferent A- or C-fibers is suppressed by lidocaine. In rats the sural nerve (skin nerve) or lateral gastrocnemius-soleus nerve (muscle nerve) was crushed. Four to 11 days after crush lesion afferent fibers were isolated from the lesioned nerves in bundles rostral to the injury site. Ongoing ectopic activity was recorded from 75 A-fibers (muscle N=43, skin N=32) and 69 C-fibers (muscle N=30, skin N=39). Most afferent fibers were functionally characterized by their responses to mechanical and thermal (mostly heat) stimuli applied at or distal to the nerve injury site. Low-threshold cold-sensitive cutaneous C-fibers were excluded from the analysis. Lidocaine was either applied to the nerve at or distal to the injury site in concentrations of 1 to 1000 ?g/mL or injected i.v. in doses of 0.09 to 9 mg/kg (skin) or 0.047 to 4.7 mg/kg (muscle). Local application of lidocaine depressed ectopic activity in A- and C-fibers dose-dependently. Depression was weaker in C- than in A-fibers. Intravenous application of lidocaine depressed ongoing ectopic activity in A- and C-fibers dose-dependently. Responses to heat or mechanical stimulation of the injured nerve were not suppressed at the highest concentrations of lidocaine. The results support the hypothesis that decrease of neuropathic pain following local or systemic application of a local anesthetic is related to decrease of ectopic ongoing activity in injured afferent nerve fibers.
AB - Lidocaine applied systemically or locally attenuates neuropathic pain in patients. Here we tested the hypothesis that ectopic activity in injured afferent A- or C-fibers is suppressed by lidocaine. In rats the sural nerve (skin nerve) or lateral gastrocnemius-soleus nerve (muscle nerve) was crushed. Four to 11 days after crush lesion afferent fibers were isolated from the lesioned nerves in bundles rostral to the injury site. Ongoing ectopic activity was recorded from 75 A-fibers (muscle N=43, skin N=32) and 69 C-fibers (muscle N=30, skin N=39). Most afferent fibers were functionally characterized by their responses to mechanical and thermal (mostly heat) stimuli applied at or distal to the nerve injury site. Low-threshold cold-sensitive cutaneous C-fibers were excluded from the analysis. Lidocaine was either applied to the nerve at or distal to the injury site in concentrations of 1 to 1000 ?g/mL or injected i.v. in doses of 0.09 to 9 mg/kg (skin) or 0.047 to 4.7 mg/kg (muscle). Local application of lidocaine depressed ectopic activity in A- and C-fibers dose-dependently. Depression was weaker in C- than in A-fibers. Intravenous application of lidocaine depressed ongoing ectopic activity in A- and C-fibers dose-dependently. Responses to heat or mechanical stimulation of the injured nerve were not suppressed at the highest concentrations of lidocaine. The results support the hypothesis that decrease of neuropathic pain following local or systemic application of a local anesthetic is related to decrease of ectopic ongoing activity in injured afferent nerve fibers.
KW - Animals
KW - Male
KW - Time Factors
KW - Disease Models, Animal
KW - Rats
KW - Statistics, Nonparametric
KW - Electric Stimulation
KW - Rats, Wistar
KW - Drug Administration Routes
KW - Action Potentials/drug effects
KW - Anesthetics, Local/administration & dosage
KW - Blood Pressure/drug effects
KW - Lidocaine/administration & dosage
KW - Nerve Fibers/drug effects/physiology
KW - Nerve Fibers, Myelinated/drug effects
KW - Nerve Fibers, Unmyelinated/drug effects
KW - Pain Threshold/drug effects
KW - Peripheral Nervous System Diseases/drug therapy/etiology
KW - Reaction Time/drug effects
KW - Skin/innervation
KW - Animals
KW - Male
KW - Time Factors
KW - Disease Models, Animal
KW - Rats
KW - Statistics, Nonparametric
KW - Electric Stimulation
KW - Rats, Wistar
KW - Drug Administration Routes
KW - Action Potentials/drug effects
KW - Anesthetics, Local/administration & dosage
KW - Blood Pressure/drug effects
KW - Lidocaine/administration & dosage
KW - Nerve Fibers/drug effects/physiology
KW - Nerve Fibers, Myelinated/drug effects
KW - Nerve Fibers, Unmyelinated/drug effects
KW - Pain Threshold/drug effects
KW - Peripheral Nervous System Diseases/drug therapy/etiology
KW - Reaction Time/drug effects
KW - Skin/innervation
M3 - SCORING: Journal article
VL - 152
SP - 1562
EP - 1571
JO - PAIN
JF - PAIN
SN - 0304-3959
IS - 7
M1 - 7
ER -
