Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow

  • René Riedel
  • Richard Addo
  • Marta Ferreira-Gomes
  • Gitta Anne Heinz
  • Frederik Heinrich
  • Jannis Kummer
  • Victor Greiff
  • Daniel Schulz
  • Cora Klaeden
  • Rebecca Cornelis
  • Ulrike Menzel
  • Stefan Kröger
  • Ulrik Stervbo
  • Ralf Köhler
  • Claudia Haftmann
  • Silvia Kühnel
  • Katrin Lehmann
  • Patrick Maschmeyer
  • Mairi McGrath
  • Sandra Naundorf
  • Stefanie Hahne
  • Özen Sercan-Alp
  • Francesco Siracusa
  • Jonathan Stefanowski
  • Melanie Weber
  • Kerstin Westendorf
  • Jakob Zimmermann
  • Anja E Hauser
  • Sai T Reddy
  • Pawel Durek
  • Hyun-Dong Chang
  • Mir-Farzin Mashreghi
  • Andreas Radbruch

Abstract

At present, it is not clear how memory B lymphocytes are maintained over time, and whether only as circulating cells or also residing in particular tissues. Here we describe distinct populations of isotype-switched memory B lymphocytes (Bsm) of murine spleen and bone marrow, identified according to individual transcriptional signature and B cell receptor repertoire. A population of marginal zone-like cells is located exclusively in the spleen, while a population of quiescent Bsm is found only in the bone marrow. Three further resident populations, present in spleen and bone marrow, represent transitional and follicular B cells and B1 cells, respectively. A population representing 10-20% of spleen and bone marrow memory B cells is the only one qualifying as circulating. In the bone marrow, all cells individually dock onto VCAM1+ stromal cells and, reminiscent of resident memory T and plasma cells, are void of activation, proliferation and mobility.

Bibliographical data

Original languageEnglish
Article number2570
ISSN2041-1723
DOIs
Publication statusPublished - 22.05.2020
PubMed 32444631