Developmental refinement of vesicle cycling at Schaffer collateral synapses

Tobias Rose; Philipp Schoenenberger; Karel Jezek; Thomas G Oertner

doi:10.1016/j.neuron.2013.01.021

Developmental refinement of vesicle cycling at Schaffer collateral synapses

Standard

Developmental refinement of vesicle cycling at Schaffer collateral synapses. / Rose, Tobias; Schoenenberger, Philipp; Jezek, Karel; Oertner, Thomas G.

In: NEURON, Vol. 77, No. 6, 20.03.2013, p. 1109-21.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rose, T, Schoenenberger, P, Jezek, K & Oertner, TG 2013, 'Developmental refinement of vesicle cycling at Schaffer collateral synapses', NEURON, vol. 77, no. 6, pp. 1109-21. https://doi.org/10.1016/j.neuron.2013.01.021

APA

Rose, T., Schoenenberger, P., Jezek, K., & Oertner, T. G. (2013). Developmental refinement of vesicle cycling at Schaffer collateral synapses. NEURON, 77(6), 1109-21. https://doi.org/10.1016/j.neuron.2013.01.021

Vancouver

Rose T, Schoenenberger P, Jezek K, Oertner TG. Developmental refinement of vesicle cycling at Schaffer collateral synapses. NEURON. 2013 Mar 20;77(6):1109-21. https://doi.org/10.1016/j.neuron.2013.01.021

Bibtex

@article{16dd5393d8224638996034eccb731703,

title = "Developmental refinement of vesicle cycling at Schaffer collateral synapses",

abstract = "At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called {"}resting pool.{"} Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.",

keywords = "Animals, Animals, Newborn, CA1 Region, Hippocampal, Endocytosis, Organ Culture Techniques, Rats, Rats, Wistar, Synapses, Synaptic Vesicles",

author = "Tobias Rose and Philipp Schoenenberger and Karel Jezek and Oertner, {Thomas G}",

year = "2013",

month = mar,

day = "20",

doi = "10.1016/j.neuron.2013.01.021",

language = "English",

volume = "77",

pages = "1109--21",

journal = "NEURON",

issn = "0896-6273",

publisher = "Cell Press",

number = "6",

}

RIS

TY - JOUR

T1 - Developmental refinement of vesicle cycling at Schaffer collateral synapses

AU - Rose, Tobias

AU - Schoenenberger, Philipp

AU - Jezek, Karel

AU - Oertner, Thomas G

PY - 2013/3/20

Y1 - 2013/3/20

N2 - At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called "resting pool." Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.

AB - At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called "resting pool." Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.

KW - Animals

KW - Animals, Newborn

KW - CA1 Region, Hippocampal

KW - Endocytosis

KW - Organ Culture Techniques

KW - Rats

KW - Rats, Wistar

KW - Synapses

KW - Synaptic Vesicles

U2 - 10.1016/j.neuron.2013.01.021

DO - 10.1016/j.neuron.2013.01.021

M3 - SCORING: Journal article

C2 - 23522046

VL - 77

SP - 1109

EP - 1121

JO - NEURON

JF - NEURON

SN - 0896-6273

IS - 6

ER -