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Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes

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Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes. / Molina, Cristina E; Llach, Anna; Alvarez-Lacalle, Enrique; Tort, Lluis; Benítez, Raul; Hove-Madsen, Leif.

In: PLOS ONE, Vol. 6, No. 8, 2011, p. e23708.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Molina, CE, Llach, A, Alvarez-Lacalle, E, Tort, L, Benítez, R & Hove-Madsen, L 2011, 'Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes', PLOS ONE, vol. 6, no. 8, pp. e23708. https://doi.org/10.1371/journal.pone.0023708

APA

Molina, C. E., Llach, A., Alvarez-Lacalle, E., Tort, L., Benítez, R., & Hove-Madsen, L. (2011). Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes. PLOS ONE, 6(8), e23708. https://doi.org/10.1371/journal.pone.0023708

Vancouver

Molina CE, Llach A, Alvarez-Lacalle E, Tort L, Benítez R, Hove-Madsen L. Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes. PLOS ONE. 2011;6(8):e23708. https://doi.org/10.1371/journal.pone.0023708

Bibtex

@article{c20dc0c438b74db796121662cb5b07d5,
title = "Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes",
abstract = "Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1.45±0.03 times the baseline fluorescence and the time to half maximal decay of sparks was 27±3 ms. Spark frequency was 0.88 sparks µm(-1) min(-1) while calcium waves were 8.5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F(0)) from 1.77±0.17 to 1.12±0.18 (p = 0.002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0.6±0.3 min(-1) to 8.1±2.0 min(-1), p = 0.001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.",
keywords = "Animals, Calcium, Calcium Signaling, Excitation Contraction Coupling, Membrane Potentials, Myocytes, Cardiac, Oncorhynchus mykiss, Patch-Clamp Techniques, Sarcoplasmic Reticulum, Species Specificity, Zebrafish, Journal Article, Research Support, Non-U.S. Gov't",
author = "Molina, {Cristina E} and Anna Llach and Enrique Alvarez-Lacalle and Lluis Tort and Raul Ben{\'i}tez and Leif Hove-Madsen",
year = "2011",
doi = "10.1371/journal.pone.0023708",
language = "English",
volume = "6",
pages = "e23708",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes

AU - Molina, Cristina E

AU - Llach, Anna

AU - Alvarez-Lacalle, Enrique

AU - Tort, Lluis

AU - Benítez, Raul

AU - Hove-Madsen, Leif

PY - 2011

Y1 - 2011

N2 - Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1.45±0.03 times the baseline fluorescence and the time to half maximal decay of sparks was 27±3 ms. Spark frequency was 0.88 sparks µm(-1) min(-1) while calcium waves were 8.5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F(0)) from 1.77±0.17 to 1.12±0.18 (p = 0.002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0.6±0.3 min(-1) to 8.1±2.0 min(-1), p = 0.001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.

AB - Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1.45±0.03 times the baseline fluorescence and the time to half maximal decay of sparks was 27±3 ms. Spark frequency was 0.88 sparks µm(-1) min(-1) while calcium waves were 8.5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F(0)) from 1.77±0.17 to 1.12±0.18 (p = 0.002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0.6±0.3 min(-1) to 8.1±2.0 min(-1), p = 0.001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.

KW - Animals

KW - Calcium

KW - Calcium Signaling

KW - Excitation Contraction Coupling

KW - Membrane Potentials

KW - Myocytes, Cardiac

KW - Oncorhynchus mykiss

KW - Patch-Clamp Techniques

KW - Sarcoplasmic Reticulum

KW - Species Specificity

KW - Zebrafish

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1371/journal.pone.0023708

DO - 10.1371/journal.pone.0023708

M3 - SCORING: Journal article

C2 - 21897853

VL - 6

SP - e23708

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 8

ER -