The HARE chip for efficient time-resolved serial synchrotron crystallography
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The HARE chip for efficient time-resolved serial synchrotron crystallography. / Mehrabi, Pedram; Müller-Werkmeister, Henrike M; Leimkohl, Jan Philipp; Schikora, Hendrik; Ninkovic, Jelena; Krivokuca, Silvia; Andriček, Ladislav; Epp, Sascha W; Sherrell, Darren; Owen, Robin L; Pearson, Arwen R; Tellkamp, Friedjof; Schulz, Eike C; Miller, R J Dwayne.
in: J SYNCHROTRON RADIAT, Jahrgang 27, Nr. Pt 2, 01.03.2020, S. 360-370.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The HARE chip for efficient time-resolved serial synchrotron crystallography
AU - Mehrabi, Pedram
AU - Müller-Werkmeister, Henrike M
AU - Leimkohl, Jan Philipp
AU - Schikora, Hendrik
AU - Ninkovic, Jelena
AU - Krivokuca, Silvia
AU - Andriček, Ladislav
AU - Epp, Sascha W
AU - Sherrell, Darren
AU - Owen, Robin L
AU - Pearson, Arwen R
AU - Tellkamp, Friedjof
AU - Schulz, Eike C
AU - Miller, R J Dwayne
N1 - open access.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.
AB - Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.
U2 - 10.1107/S1600577520000685
DO - 10.1107/S1600577520000685
M3 - SCORING: Journal article
C2 - 32153274
VL - 27
SP - 360
EP - 370
JO - J SYNCHROTRON RADIAT
JF - J SYNCHROTRON RADIAT
SN - 0909-0495
IS - Pt 2
ER -