Role of the postnatal radial glial scaffold for the development of the dentate gyrus as revealed by Reelin signaling mutant mice
Standard
Role of the postnatal radial glial scaffold for the development of the dentate gyrus as revealed by Reelin signaling mutant mice. / Brunne, Bianka; Franco, Santos; Bouché, Elisabeth; Herz, Joachim; Howell, Brian W; Pahle, Jasmine; Müller, Ulrich; May, Petra; Frotscher, Michael; Bock, Hans H.
in: GLIA, Jahrgang 61, Nr. 8, 01.08.2013, S. 1347-63.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Role of the postnatal radial glial scaffold for the development of the dentate gyrus as revealed by Reelin signaling mutant mice
AU - Brunne, Bianka
AU - Franco, Santos
AU - Bouché, Elisabeth
AU - Herz, Joachim
AU - Howell, Brian W
AU - Pahle, Jasmine
AU - Müller, Ulrich
AU - May, Petra
AU - Frotscher, Michael
AU - Bock, Hans H
N1 - Copyright © 2013 Wiley Periodicals, Inc.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - During dentate gyrus development, the early embryonic radial glial scaffold is replaced by a secondary glial scaffold around birth. In contrast to neocortical and early dentate gyrus radial glial cells, these postnatal glial cells are severely altered with regard to position and morphology in reeler mice lacking the secreted protein Reelin. In this study, we focus on the functional impact of these defects. Most radial glial cells throughout the nervous system serve as scaffolds for migrating neurons and precursor cells for both neurogenesis and gliogenesis. Precursor cell function has been demonstrated for secondary radial glial cells but the exact function of these late glial cells in granule cell migration and positioning is not clear. No data exist concerning the interplay between granule neurons and late radial glial cells during dentate gyrus development. Herein, we show that despite the severe morphological defects in the reeler dentate gyrus, the precursor function of secondary radial glial cells is not impaired during development in reeler mice. In addition, selective ablation of Disabled-1, an intracellular adaptor protein essential for Reelin signaling, in neurons but not in glial cells allowed us to distinguish effects of Reelin signaling on radial glial cells from possible secondary effects based on defective granule cells positioning.
AB - During dentate gyrus development, the early embryonic radial glial scaffold is replaced by a secondary glial scaffold around birth. In contrast to neocortical and early dentate gyrus radial glial cells, these postnatal glial cells are severely altered with regard to position and morphology in reeler mice lacking the secreted protein Reelin. In this study, we focus on the functional impact of these defects. Most radial glial cells throughout the nervous system serve as scaffolds for migrating neurons and precursor cells for both neurogenesis and gliogenesis. Precursor cell function has been demonstrated for secondary radial glial cells but the exact function of these late glial cells in granule cell migration and positioning is not clear. No data exist concerning the interplay between granule neurons and late radial glial cells during dentate gyrus development. Herein, we show that despite the severe morphological defects in the reeler dentate gyrus, the precursor function of secondary radial glial cells is not impaired during development in reeler mice. In addition, selective ablation of Disabled-1, an intracellular adaptor protein essential for Reelin signaling, in neurons but not in glial cells allowed us to distinguish effects of Reelin signaling on radial glial cells from possible secondary effects based on defective granule cells positioning.
KW - Animals
KW - Animals, Newborn
KW - Cell Adhesion Molecules, Neuronal
KW - Cells, Cultured
KW - Dentate Gyrus
KW - Ependymoglial Cells
KW - Extracellular Matrix Proteins
KW - Mice
KW - Mice, 129 Strain
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - Mice, Neurologic Mutants
KW - Mutation
KW - Nerve Tissue Proteins
KW - Phenotype
KW - Recombinant Proteins
KW - Serine Endopeptidases
KW - Signal Transduction
KW - Stem Cells
U2 - 10.1002/glia.22519
DO - 10.1002/glia.22519
M3 - SCORING: Journal article
C2 - 23828756
VL - 61
SP - 1347
EP - 1363
JO - GLIA
JF - GLIA
SN - 0894-1491
IS - 8
ER -