Role for Escherichia coli YidD in membrane protein insertion
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Role for Escherichia coli YidD in membrane protein insertion. / Yu, Zhong; Lavèn, Mariëlle; Klepsch, Mirjam; de Gier, Jan-Willem; Bitter, Wilbert; van Ulsen, Peter; Luirink, Joen.
in: Journal of bacteriology, Jahrgang 193, Nr. 19, 10.2011, S. 5242-51.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Role for Escherichia coli YidD in membrane protein insertion
AU - Yu, Zhong
AU - Lavèn, Mariëlle
AU - Klepsch, Mirjam
AU - de Gier, Jan-Willem
AU - Bitter, Wilbert
AU - van Ulsen, Peter
AU - Luirink, Joen
PY - 2011/10
Y1 - 2011/10
N2 - YidC has an essential but poorly defined function in membrane protein insertion and folding in bacteria. The yidC gene is located in a gene cluster that is highly conserved in Gram-negative bacteria, the gene order being rpmH, rnpA, yidD, yidC, and trmE. Here, we show that Escherichia coli yidD, which overlaps with rnpA and is only 2 bp upstream of yidC, is expressed and localizes to the inner membrane, probably through an amphipathic helix. Inactivation of yidD had no discernible effect on cell growth and viability. However, compared to control cells, ΔyidD cells were affected in the insertion and processing of three YidC-dependent inner membrane proteins. Furthermore, in vitro cross-linking showed that YidD is in proximity of a nascent inner membrane protein during its localization in the Sec-YidC translocon, suggesting that YidD might be involved in the insertion process.
AB - YidC has an essential but poorly defined function in membrane protein insertion and folding in bacteria. The yidC gene is located in a gene cluster that is highly conserved in Gram-negative bacteria, the gene order being rpmH, rnpA, yidD, yidC, and trmE. Here, we show that Escherichia coli yidD, which overlaps with rnpA and is only 2 bp upstream of yidC, is expressed and localizes to the inner membrane, probably through an amphipathic helix. Inactivation of yidD had no discernible effect on cell growth and viability. However, compared to control cells, ΔyidD cells were affected in the insertion and processing of three YidC-dependent inner membrane proteins. Furthermore, in vitro cross-linking showed that YidD is in proximity of a nascent inner membrane protein during its localization in the Sec-YidC translocon, suggesting that YidD might be involved in the insertion process.
KW - Amino Acid Sequence
KW - Cell Membrane
KW - Escherichia coli
KW - Escherichia coli Proteins
KW - Membrane Proteins
KW - Membrane Transport Proteins
KW - Microscopy, Fluorescence
KW - Molecular Sequence Data
KW - Protein Transport
KW - Sequence Homology, Amino Acid
KW - Journal Article
U2 - 10.1128/JB.05429-11
DO - 10.1128/JB.05429-11
M3 - SCORING: Journal article
C2 - 21803992
VL - 193
SP - 5242
EP - 5251
JO - J BACTERIOL
JF - J BACTERIOL
SN - 0021-9193
IS - 19
ER -