Region-specific alteration of GABAergic markers in the brain of heterozygous reeler mice.
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Region-specific alteration of GABAergic markers in the brain of heterozygous reeler mice. / Nullmeier, S; Panther, P; Dobrowolny, H; Frotscher, Michael; Zhao, S; Schwegler, H; Wolf, R.
in: EUR J NEUROSCI, Jahrgang 33, Nr. 4, 4, 2011, S. 689-698.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Region-specific alteration of GABAergic markers in the brain of heterozygous reeler mice.
AU - Nullmeier, S
AU - Panther, P
AU - Dobrowolny, H
AU - Frotscher, Michael
AU - Zhao, S
AU - Schwegler, H
AU - Wolf, R
PY - 2011
Y1 - 2011
N2 - Heterozygous reeler mice (HRM), haploinsufficient for reelin, have been proposed to be a genetic mouse model of schizophrenia. Beside behavioural similarities, HRM also demonstrate several neuroanatomical traits similar to patients suffering from schizophrenia. In the present study using immunocytochemical procedures, we investigated HRM and wild-type mice (WT) for differences in the numbers and densities of glutamic acid decarboxylase (GAD)67 and parvalbumin (PARV)-immunoreactive (IR) neurons in the hippocampus, tyrosine hydroxylase (TH)-IR neurons in the ventral tegmental area (VTA) and substantia nigra (SN), and serotonin transporter (5-HT-T)-IR neurons of the raphe nuclei. We found that HRM, compared with WT, show a significant decrease of GAD67-IR neurons in hippocampal subregion CA1 [stratum pyramidale (SP)], CA2 [stratum oriens (SO), stratum pyramidale (SP) and stratum radiatum (SR)] and dentate gyrus [granule cell layer (GL)], and also a significant decrease of PARV-containing neurons in CA1 (SO, SP) and CA2 (SP). No morphological differences were found in the SN/VTA or raphe nuclei. In conclusion, these results support a hippocampal ?-aminobutyric acid (GABA)ergic dysfunction in HRM as previously described by other authors, and may be based on a downregulation of GAD67 and PARV expressions. In summary, the reelin haploinsufficient mouse may provide a useful model for studying the interaction between reelin and hippocampal GABAergic system, its effect on dendritic spine maturation and plasticity related to schizophrenia.
AB - Heterozygous reeler mice (HRM), haploinsufficient for reelin, have been proposed to be a genetic mouse model of schizophrenia. Beside behavioural similarities, HRM also demonstrate several neuroanatomical traits similar to patients suffering from schizophrenia. In the present study using immunocytochemical procedures, we investigated HRM and wild-type mice (WT) for differences in the numbers and densities of glutamic acid decarboxylase (GAD)67 and parvalbumin (PARV)-immunoreactive (IR) neurons in the hippocampus, tyrosine hydroxylase (TH)-IR neurons in the ventral tegmental area (VTA) and substantia nigra (SN), and serotonin transporter (5-HT-T)-IR neurons of the raphe nuclei. We found that HRM, compared with WT, show a significant decrease of GAD67-IR neurons in hippocampal subregion CA1 [stratum pyramidale (SP)], CA2 [stratum oriens (SO), stratum pyramidale (SP) and stratum radiatum (SR)] and dentate gyrus [granule cell layer (GL)], and also a significant decrease of PARV-containing neurons in CA1 (SO, SP) and CA2 (SP). No morphological differences were found in the SN/VTA or raphe nuclei. In conclusion, these results support a hippocampal ?-aminobutyric acid (GABA)ergic dysfunction in HRM as previously described by other authors, and may be based on a downregulation of GAD67 and PARV expressions. In summary, the reelin haploinsufficient mouse may provide a useful model for studying the interaction between reelin and hippocampal GABAergic system, its effect on dendritic spine maturation and plasticity related to schizophrenia.
KW - Animals
KW - Humans
KW - Male
KW - Female
KW - Mice
KW - Cell Adhesion Molecules, Neuronal/genetics/metabolism
KW - Nerve Tissue Proteins/genetics/metabolism
KW - Neurons/cytology/metabolism
KW - Biological Markers/metabolism
KW - Brain/anatomy & histology/metabolism
KW - Extracellular Matrix Proteins/genetics/metabolism
KW - Glutamate Decarboxylase/genetics/metabolism
KW - Mice, Neurologic Mutants
KW - Schizophrenia/physiopathology
KW - Serine Endopeptidases/genetics/metabolism
KW - Tyrosine 3-Monooxygenase/metabolism
KW - gamma-Aminobutyric Acid/metabolism
KW - Animals
KW - Humans
KW - Male
KW - Female
KW - Mice
KW - Cell Adhesion Molecules, Neuronal/genetics/metabolism
KW - Nerve Tissue Proteins/genetics/metabolism
KW - Neurons/cytology/metabolism
KW - Biological Markers/metabolism
KW - Brain/anatomy & histology/metabolism
KW - Extracellular Matrix Proteins/genetics/metabolism
KW - Glutamate Decarboxylase/genetics/metabolism
KW - Mice, Neurologic Mutants
KW - Schizophrenia/physiopathology
KW - Serine Endopeptidases/genetics/metabolism
KW - Tyrosine 3-Monooxygenase/metabolism
KW - gamma-Aminobutyric Acid/metabolism
M3 - SCORING: Journal article
VL - 33
SP - 689
EP - 698
JO - EUR J NEUROSCI
JF - EUR J NEUROSCI
SN - 0953-816X
IS - 4
M1 - 4
ER -