Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells

Sebastian Thaler; Johanna Hofmann; Karl-Ulrich Bartz-Schmidt; Frank Schuettauf; Christos Haritoglou; Efdal Yoeruek

doi:10.1016/j.jcrs.2010.12.051

Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery

Standard

Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery : capsule staining properties and cytotoxicity to human cultured corneal endothelial cells. / Thaler, Sebastian; Hofmann, Johanna; Bartz-Schmidt, Karl-Ulrich; Schuettauf, Frank; Haritoglou, Christos; Yoeruek, Efdal.

in: J CATARACT REFR SURG, Jahrgang 37, Nr. 6, 06.2011, S. 1147-53.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Thaler, S, Hofmann, J, Bartz-Schmidt, K-U, Schuettauf, F, Haritoglou, C & Yoeruek, E 2011, 'Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells', J CATARACT REFR SURG, Jg. 37, Nr. 6, S. 1147-53. https://doi.org/10.1016/j.jcrs.2010.12.051

APA

Thaler, S., Hofmann, J., Bartz-Schmidt, K-U., Schuettauf, F., Haritoglou, C., & Yoeruek, E. (2011). Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells. J CATARACT REFR SURG, 37(6), 1147-53. https://doi.org/10.1016/j.jcrs.2010.12.051

Vancouver

Thaler S, Hofmann J, Bartz-Schmidt K-U, Schuettauf F, Haritoglou C, Yoeruek E. Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells. J CATARACT REFR SURG. 2011 Jun;37(6):1147-53. https://doi.org/10.1016/j.jcrs.2010.12.051

Bibtex

@article{32b7ad5e03c940959af4ca1b0b2d5783,

title = "Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells",

abstract = "PURPOSE: To evaluate capsule-staining properties and biocompatibility of the triarylmethane dyes methyl blue and aniline blue compared with patent blue and trypan blue on cultured human corneal endothelial cells.SETTING: Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany.DESIGN: Experimental study.METHODS: Human corneal endothelial cell cultures were harvested from human donor cells and exposed to various concentrations (0.025 to 5.0 mg/mL) of methyl blue, aniline blue, patent blue, and trypan blue. Cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test after 24 hours of incubation. Calcein live cell staining was performed at the same time point. The dyes were also used to stain pig lens capsules in vitro by incubating the lenses for 1 minute with 3 concentrations (0.5, 1.5, and 2.5 mg/mL) of dye, after which the staining properties were evaluated.RESULTS: No significant cytotoxicity was detected for patent blue and methyl blue at any tested concentration. However, aniline blue exerted significant cytotoxicity at concentrations of 1.5 mg/mL or higher and trypan blue at 2.5 mg/mL or higher. Capsule staining of the tested triarylmethane dyes was suitable for performing capsulorhexis, but only at higher concentrations than with trypan blue.CONCLUSIONS: High concentrations and long incubation times of trypan blue and aniline blue showed significant cytotoxicity to human cultured endothelial cells in contrast to patent blue and methyl blue. All tested dyes were able to stain lens capsules sufficiently for capsulorhexis creation.FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.",

keywords = "Aniline Compounds/toxicity, Animals, Benzenesulfonates/toxicity, Cataract Extraction, Cells, Cultured, Coloring Agents/toxicity, Endothelium, Corneal/drug effects, Fluoresceins/metabolism, Formazans, Humans, Lens Capsule, Crystalline/anatomy & histology, Rosaniline Dyes/toxicity, Staining and Labeling/methods, Swine, Tetrazolium Salts, Trypan Blue/toxicity",

author = "Sebastian Thaler and Johanna Hofmann and Karl-Ulrich Bartz-Schmidt and Frank Schuettauf and Christos Haritoglou and Efdal Yoeruek",

year = "2011",

month = jun,

doi = "10.1016/j.jcrs.2010.12.051",

language = "English",

volume = "37",

pages = "1147--53",

journal = "J CATARACT REFR SURG",

issn = "0886-3350",

publisher = "Elsevier Inc.",

number = "6",

}

RIS

TY - JOUR

T1 - Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery

T2 - capsule staining properties and cytotoxicity to human cultured corneal endothelial cells

AU - Thaler, Sebastian

AU - Hofmann, Johanna

AU - Bartz-Schmidt, Karl-Ulrich

AU - Schuettauf, Frank

AU - Haritoglou, Christos

AU - Yoeruek, Efdal

PY - 2011/6

Y1 - 2011/6

N2 - PURPOSE: To evaluate capsule-staining properties and biocompatibility of the triarylmethane dyes methyl blue and aniline blue compared with patent blue and trypan blue on cultured human corneal endothelial cells.SETTING: Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany.DESIGN: Experimental study.METHODS: Human corneal endothelial cell cultures were harvested from human donor cells and exposed to various concentrations (0.025 to 5.0 mg/mL) of methyl blue, aniline blue, patent blue, and trypan blue. Cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test after 24 hours of incubation. Calcein live cell staining was performed at the same time point. The dyes were also used to stain pig lens capsules in vitro by incubating the lenses for 1 minute with 3 concentrations (0.5, 1.5, and 2.5 mg/mL) of dye, after which the staining properties were evaluated.RESULTS: No significant cytotoxicity was detected for patent blue and methyl blue at any tested concentration. However, aniline blue exerted significant cytotoxicity at concentrations of 1.5 mg/mL or higher and trypan blue at 2.5 mg/mL or higher. Capsule staining of the tested triarylmethane dyes was suitable for performing capsulorhexis, but only at higher concentrations than with trypan blue.CONCLUSIONS: High concentrations and long incubation times of trypan blue and aniline blue showed significant cytotoxicity to human cultured endothelial cells in contrast to patent blue and methyl blue. All tested dyes were able to stain lens capsules sufficiently for capsulorhexis creation.FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.

AB - PURPOSE: To evaluate capsule-staining properties and biocompatibility of the triarylmethane dyes methyl blue and aniline blue compared with patent blue and trypan blue on cultured human corneal endothelial cells.SETTING: Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany.DESIGN: Experimental study.METHODS: Human corneal endothelial cell cultures were harvested from human donor cells and exposed to various concentrations (0.025 to 5.0 mg/mL) of methyl blue, aniline blue, patent blue, and trypan blue. Cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test after 24 hours of incubation. Calcein live cell staining was performed at the same time point. The dyes were also used to stain pig lens capsules in vitro by incubating the lenses for 1 minute with 3 concentrations (0.5, 1.5, and 2.5 mg/mL) of dye, after which the staining properties were evaluated.RESULTS: No significant cytotoxicity was detected for patent blue and methyl blue at any tested concentration. However, aniline blue exerted significant cytotoxicity at concentrations of 1.5 mg/mL or higher and trypan blue at 2.5 mg/mL or higher. Capsule staining of the tested triarylmethane dyes was suitable for performing capsulorhexis, but only at higher concentrations than with trypan blue.CONCLUSIONS: High concentrations and long incubation times of trypan blue and aniline blue showed significant cytotoxicity to human cultured endothelial cells in contrast to patent blue and methyl blue. All tested dyes were able to stain lens capsules sufficiently for capsulorhexis creation.FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.

KW - Aniline Compounds/toxicity

KW - Animals

KW - Benzenesulfonates/toxicity

KW - Cataract Extraction

KW - Cells, Cultured

KW - Coloring Agents/toxicity

KW - Endothelium, Corneal/drug effects

KW - Fluoresceins/metabolism

KW - Formazans

KW - Humans

KW - Lens Capsule, Crystalline/anatomy & histology

KW - Rosaniline Dyes/toxicity

KW - Staining and Labeling/methods

KW - Swine

KW - Tetrazolium Salts

KW - Trypan Blue/toxicity

U2 - 10.1016/j.jcrs.2010.12.051

DO - 10.1016/j.jcrs.2010.12.051

M3 - SCORING: Journal article

C2 - 21596258

VL - 37

SP - 1147

EP - 1153

JO - J CATARACT REFR SURG

JF - J CATARACT REFR SURG

SN - 0886-3350

IS - 6

ER -