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Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin

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Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin. / Chua, Melvin Lee Kiang; Somaiah, Navita; Bourne, Sara; Daley, Frances; A'hern, Roger; Nuta, Otilia; Davies, Sue; Herskind, Carsten; Pearson, Ann; Warrington, Jim; Helyer, Sarah; Owen, Roger; Yarnold, John; Rothkamm, Kai.

in: RADIOTHER ONCOL, Jahrgang 99, Nr. 2, 05.2011, S. 225-30.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Chua, MLK, Somaiah, N, Bourne, S, Daley, F, A'hern, R, Nuta, O, Davies, S, Herskind, C, Pearson, A, Warrington, J, Helyer, S, Owen, R, Yarnold, J & Rothkamm, K 2011, 'Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin', RADIOTHER ONCOL, Jg. 99, Nr. 2, S. 225-30. https://doi.org/10.1016/j.radonc.2011.04.009

APA

Chua, M. L. K., Somaiah, N., Bourne, S., Daley, F., A'hern, R., Nuta, O., Davies, S., Herskind, C., Pearson, A., Warrington, J., Helyer, S., Owen, R., Yarnold, J., & Rothkamm, K. (2011). Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin. RADIOTHER ONCOL, 99(2), 225-30. https://doi.org/10.1016/j.radonc.2011.04.009

Vancouver

Chua MLK, Somaiah N, Bourne S, Daley F, A'hern R, Nuta O et al. Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin. RADIOTHER ONCOL. 2011 Mai;99(2):225-30. https://doi.org/10.1016/j.radonc.2011.04.009

Bibtex

@article{0f198003b8be41dcbbd1cf9462297f07,
title = "Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin",
abstract = "PURPOSE: The aim of this study was to compare inter-individual and inter-cell type variation in DNA double-strand break (DSB) repair following in vivo irradiation of human skin.MATERIALS AND METHODS: Duplicate 4mm core biopsies of irradiated and unirradiated skin were collected from 35 patients 24h after 4Gy exposure using 6MeV electrons. Residual DSB were quantified by scoring 53BP1 foci in dermal fibroblasts, endothelial cells, superficial keratinocytes and basal epidermal cells.RESULTS: Coefficients of inter-individual variation for levels of residual foci 24h after in vivo irradiation of skin were 39.9% in dermal fibroblasts, 44.3% in endothelial cells, 32.9% in superficial keratinocytes and 46.4% in basal epidermal cells (p<0.001, ANOVA). In contrast, the coefficient of inter-cell type variation for residual foci levels was only 11.3% in human skin between the different epidermal and dermal cells (p=0.034, ANOVA). Foci levels between the different skin cell types were correlated (Pearson's R=0.855-0.955, p<0.001).CONCLUSIONS: Patient-specific factors appear to be more important than cell type-specific factors in determining residual foci levels following in vivo irradiation of human skin.",
keywords = "Aged, Analysis of Variance, Biopsy, Breast Neoplasms/radiotherapy, DNA Breaks, Double-Stranded/radiation effects, Dose-Response Relationship, Radiation, Endothelial Cells/radiation effects, Female, Fibroblasts/radiation effects, Humans, Immunoenzyme Techniques, Keratinocytes/radiation effects, Radiation Injuries/genetics, Skin/cytology",
author = "Chua, {Melvin Lee Kiang} and Navita Somaiah and Sara Bourne and Frances Daley and Roger A'hern and Otilia Nuta and Sue Davies and Carsten Herskind and Ann Pearson and Jim Warrington and Sarah Helyer and Roger Owen and John Yarnold and Kai Rothkamm",
note = "Copyright {\textcopyright} 2011 Elsevier Ireland Ltd. All rights reserved.",
year = "2011",
month = may,
doi = "10.1016/j.radonc.2011.04.009",
language = "English",
volume = "99",
pages = "225--30",
journal = "RADIOTHER ONCOL",
issn = "0167-8140",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Inter-individual and inter-cell type variation in residual DNA damage after in vivo irradiation of human skin

AU - Chua, Melvin Lee Kiang

AU - Somaiah, Navita

AU - Bourne, Sara

AU - Daley, Frances

AU - A'hern, Roger

AU - Nuta, Otilia

AU - Davies, Sue

AU - Herskind, Carsten

AU - Pearson, Ann

AU - Warrington, Jim

AU - Helyer, Sarah

AU - Owen, Roger

AU - Yarnold, John

AU - Rothkamm, Kai

N1 - Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

PY - 2011/5

Y1 - 2011/5

N2 - PURPOSE: The aim of this study was to compare inter-individual and inter-cell type variation in DNA double-strand break (DSB) repair following in vivo irradiation of human skin.MATERIALS AND METHODS: Duplicate 4mm core biopsies of irradiated and unirradiated skin were collected from 35 patients 24h after 4Gy exposure using 6MeV electrons. Residual DSB were quantified by scoring 53BP1 foci in dermal fibroblasts, endothelial cells, superficial keratinocytes and basal epidermal cells.RESULTS: Coefficients of inter-individual variation for levels of residual foci 24h after in vivo irradiation of skin were 39.9% in dermal fibroblasts, 44.3% in endothelial cells, 32.9% in superficial keratinocytes and 46.4% in basal epidermal cells (p<0.001, ANOVA). In contrast, the coefficient of inter-cell type variation for residual foci levels was only 11.3% in human skin between the different epidermal and dermal cells (p=0.034, ANOVA). Foci levels between the different skin cell types were correlated (Pearson's R=0.855-0.955, p<0.001).CONCLUSIONS: Patient-specific factors appear to be more important than cell type-specific factors in determining residual foci levels following in vivo irradiation of human skin.

AB - PURPOSE: The aim of this study was to compare inter-individual and inter-cell type variation in DNA double-strand break (DSB) repair following in vivo irradiation of human skin.MATERIALS AND METHODS: Duplicate 4mm core biopsies of irradiated and unirradiated skin were collected from 35 patients 24h after 4Gy exposure using 6MeV electrons. Residual DSB were quantified by scoring 53BP1 foci in dermal fibroblasts, endothelial cells, superficial keratinocytes and basal epidermal cells.RESULTS: Coefficients of inter-individual variation for levels of residual foci 24h after in vivo irradiation of skin were 39.9% in dermal fibroblasts, 44.3% in endothelial cells, 32.9% in superficial keratinocytes and 46.4% in basal epidermal cells (p<0.001, ANOVA). In contrast, the coefficient of inter-cell type variation for residual foci levels was only 11.3% in human skin between the different epidermal and dermal cells (p=0.034, ANOVA). Foci levels between the different skin cell types were correlated (Pearson's R=0.855-0.955, p<0.001).CONCLUSIONS: Patient-specific factors appear to be more important than cell type-specific factors in determining residual foci levels following in vivo irradiation of human skin.

KW - Aged

KW - Analysis of Variance

KW - Biopsy

KW - Breast Neoplasms/radiotherapy

KW - DNA Breaks, Double-Stranded/radiation effects

KW - Dose-Response Relationship, Radiation

KW - Endothelial Cells/radiation effects

KW - Female

KW - Fibroblasts/radiation effects

KW - Humans

KW - Immunoenzyme Techniques

KW - Keratinocytes/radiation effects

KW - Radiation Injuries/genetics

KW - Skin/cytology

U2 - 10.1016/j.radonc.2011.04.009

DO - 10.1016/j.radonc.2011.04.009

M3 - SCORING: Journal article

C2 - 21620495

VL - 99

SP - 225

EP - 230

JO - RADIOTHER ONCOL

JF - RADIOTHER ONCOL

SN - 0167-8140

IS - 2

ER -