Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells
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Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells. / Fortmann, Ingmar; Dammann, Marie-Theres; Siller, Bastian; Humberg, Alexander; Demmert, Martin; Tüshaus, Ludger; Lindert, Judith; van Zandbergen, Vera; Pagel, Julia; Rupp, Jan; Herting, Egbert; Härtel, Christoph.
in: FRONT IMMUNOL, Jahrgang 12, 2021, S. 666447.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells
AU - Fortmann, Ingmar
AU - Dammann, Marie-Theres
AU - Siller, Bastian
AU - Humberg, Alexander
AU - Demmert, Martin
AU - Tüshaus, Ludger
AU - Lindert, Judith
AU - van Zandbergen, Vera
AU - Pagel, Julia
AU - Rupp, Jan
AU - Herting, Egbert
AU - Härtel, Christoph
N1 - Copyright © 2021 Fortmann, Dammann, Siller, Humberg, Demmert, Tüshaus, Lindert, van Zandbergen, Pagel, Rupp, Herting and Härtel.
PY - 2021
Y1 - 2021
N2 - OBJECTIVE: To provide epidemiological data of infants < 90 days of age with suspected late-onset sepsis (LOS) and evaluate distinct immunological specificities. We hypothesized that previously healthy infants < 3 months of age with sepsis have a yet undefined immunological predisposition; e.g. differences in lymphocyte subsets including regulatory T cells.METHODS: We performed an exploratory, single center study between January 1st, 2019 and June 1st, 2021. Routine diagnostics included conventional culture (blood, cerebrospinal fluid, urine), PCR and inflammatory markers in infants < 90 days of age with suspected sepsis. We additionally analyzed lymphocyte subsets and CD4+ CD25+ forkhead box protein (FoxP3)+ Tregs at admission for sepsis workup as compared to age-matched controls.RESULTS: A convenience sample cohort of n= 51 infants with sepsis workup was enrolled. Invasive bacterial infection (IBI) was diagnosed in 25 (49.0%) patients including two infants with a rhinovirus co-infection and viral infection in 14 (27.5%) neonates. No infectious cause was found in 12 cases. Infants with suspected LOS displayed a decreased abundance of CD4+ FoxP3+ T cells as compared to controls, which was most pronounced in the subgroup of infants with IBI. We also noticed elevated HLA-DR-positive CD3+ cells in infants with LOS and a higher CD4/CD8-ratio in infants with viral infection as compared to healthy controls. Infants with viral infections had a higher number of natural killer cells as compared to infants with IBI.CONCLUSION: Our exploratory data support the concept of a potential immaturity state and failed immune tolerance development for young infants with LOS. Future large-scale studies are needed to elucidate pre-sepsis conditions and to target the microbiome-immunity interplay as a potential risk pattern.
AB - OBJECTIVE: To provide epidemiological data of infants < 90 days of age with suspected late-onset sepsis (LOS) and evaluate distinct immunological specificities. We hypothesized that previously healthy infants < 3 months of age with sepsis have a yet undefined immunological predisposition; e.g. differences in lymphocyte subsets including regulatory T cells.METHODS: We performed an exploratory, single center study between January 1st, 2019 and June 1st, 2021. Routine diagnostics included conventional culture (blood, cerebrospinal fluid, urine), PCR and inflammatory markers in infants < 90 days of age with suspected sepsis. We additionally analyzed lymphocyte subsets and CD4+ CD25+ forkhead box protein (FoxP3)+ Tregs at admission for sepsis workup as compared to age-matched controls.RESULTS: A convenience sample cohort of n= 51 infants with sepsis workup was enrolled. Invasive bacterial infection (IBI) was diagnosed in 25 (49.0%) patients including two infants with a rhinovirus co-infection and viral infection in 14 (27.5%) neonates. No infectious cause was found in 12 cases. Infants with suspected LOS displayed a decreased abundance of CD4+ FoxP3+ T cells as compared to controls, which was most pronounced in the subgroup of infants with IBI. We also noticed elevated HLA-DR-positive CD3+ cells in infants with LOS and a higher CD4/CD8-ratio in infants with viral infection as compared to healthy controls. Infants with viral infections had a higher number of natural killer cells as compared to infants with IBI.CONCLUSION: Our exploratory data support the concept of a potential immaturity state and failed immune tolerance development for young infants with LOS. Future large-scale studies are needed to elucidate pre-sepsis conditions and to target the microbiome-immunity interplay as a potential risk pattern.
KW - Age of Onset
KW - Bacterial Infections/microbiology
KW - Cohort Studies
KW - Communicable Diseases
KW - Female
KW - Forkhead Transcription Factors/blood
KW - Gestational Age
KW - Humans
KW - Immune Tolerance
KW - Infant
KW - Infant, Newborn
KW - Lymphocyte Subsets/cytology
KW - Male
KW - Sepsis/immunology
KW - T-Lymphocytes, Regulatory/immunology
U2 - 10.3389/fimmu.2021.666447
DO - 10.3389/fimmu.2021.666447
M3 - SCORING: Journal article
C2 - 34512621
VL - 12
SP - 666447
JO - FRONT IMMUNOL
JF - FRONT IMMUNOL
SN - 1664-3224
ER -
