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Human and Murine T-Helper Cell Recovery from Organs and Tissues

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Human and Murine T-Helper Cell Recovery from Organs and Tissues. / Siracusa, Francesco; Lahmann, Annette; Vanni, Anna; Cortesi, Filippo; Mazzoni, Alessio.

in: Methods Mol Biol, Jahrgang 2285, 2021, S. 1-25.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Siracusa, F, Lahmann, A, Vanni, A, Cortesi, F & Mazzoni, A 2021, 'Human and Murine T-Helper Cell Recovery from Organs and Tissues', Methods Mol Biol, Jg. 2285, S. 1-25. https://doi.org/10.1007/978-1-0716-1311-5_1

APA

Siracusa, F., Lahmann, A., Vanni, A., Cortesi, F., & Mazzoni, A. (2021). Human and Murine T-Helper Cell Recovery from Organs and Tissues. Methods Mol Biol, 2285, 1-25. https://doi.org/10.1007/978-1-0716-1311-5_1

Vancouver

Siracusa F, Lahmann A, Vanni A, Cortesi F, Mazzoni A. Human and Murine T-Helper Cell Recovery from Organs and Tissues. Methods Mol Biol. 2021;2285:1-25. https://doi.org/10.1007/978-1-0716-1311-5_1

Bibtex

@article{132a4cdc9a9140538e213abd4b0bffc1,
title = "Human and Murine T-Helper Cell Recovery from Organs and Tissues",
abstract = "Isolation of cells from organs and tissues represents a challenge for many researchers. Cell yield, purity, and cell death are common problems associated with it, which greatly affect experimental results in terms of reproducibility and biological observations. Here, we describe state-of-the-art protocols of how to isolate CD4+ T cells from both human and murine organs and tissues, reducing at minimum cell death, while increasing at the same time cell yield and purity.",
keywords = "Animals, Biomarkers/metabolism, Cell Separation, Cell Survival, Enzymes/metabolism, Flow Cytometry, Humans, Immunomagnetic Separation, Lymphoid Tissue/cytology, Mice, Inbred C57BL, Phenotype, Research Design, T-Lymphocytes, Helper-Inducer/immunology, Workflow",
author = "Francesco Siracusa and Annette Lahmann and Anna Vanni and Filippo Cortesi and Alessio Mazzoni",
year = "2021",
doi = "10.1007/978-1-0716-1311-5_1",
language = "English",
volume = "2285",
pages = "1--25",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Human and Murine T-Helper Cell Recovery from Organs and Tissues

AU - Siracusa, Francesco

AU - Lahmann, Annette

AU - Vanni, Anna

AU - Cortesi, Filippo

AU - Mazzoni, Alessio

PY - 2021

Y1 - 2021

N2 - Isolation of cells from organs and tissues represents a challenge for many researchers. Cell yield, purity, and cell death are common problems associated with it, which greatly affect experimental results in terms of reproducibility and biological observations. Here, we describe state-of-the-art protocols of how to isolate CD4+ T cells from both human and murine organs and tissues, reducing at minimum cell death, while increasing at the same time cell yield and purity.

AB - Isolation of cells from organs and tissues represents a challenge for many researchers. Cell yield, purity, and cell death are common problems associated with it, which greatly affect experimental results in terms of reproducibility and biological observations. Here, we describe state-of-the-art protocols of how to isolate CD4+ T cells from both human and murine organs and tissues, reducing at minimum cell death, while increasing at the same time cell yield and purity.

KW - Animals

KW - Biomarkers/metabolism

KW - Cell Separation

KW - Cell Survival

KW - Enzymes/metabolism

KW - Flow Cytometry

KW - Humans

KW - Immunomagnetic Separation

KW - Lymphoid Tissue/cytology

KW - Mice, Inbred C57BL

KW - Phenotype

KW - Research Design

KW - T-Lymphocytes, Helper-Inducer/immunology

KW - Workflow

U2 - 10.1007/978-1-0716-1311-5_1

DO - 10.1007/978-1-0716-1311-5_1

M3 - SCORING: Journal article

C2 - 33928539

VL - 2285

SP - 1

EP - 25

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -