High expression of survivin and down-regulation of Stat-3 characterize the feto-maternal interface in failing murine pregnancies during the implantation period
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High expression of survivin and down-regulation of Stat-3 characterize the feto-maternal interface in failing murine pregnancies during the implantation period. / Garcia, Mariana G; Tirado-Gonzalez, Irene; Handjiski, Bori; Tometten, Mareike; Orsal, Arif S; Hajos, Silvia E; Fernández, Nelson; Arck, Petra C; Blois, Sandra M.
in: PLACENTA, Jahrgang 28, Nr. 7, 07.2007, S. 650-7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - High expression of survivin and down-regulation of Stat-3 characterize the feto-maternal interface in failing murine pregnancies during the implantation period
AU - Garcia, Mariana G
AU - Tirado-Gonzalez, Irene
AU - Handjiski, Bori
AU - Tometten, Mareike
AU - Orsal, Arif S
AU - Hajos, Silvia E
AU - Fernández, Nelson
AU - Arck, Petra C
AU - Blois, Sandra M
PY - 2007/7
Y1 - 2007/7
N2 - The materno-fetal interface has for long been considered as an immune privileged biological site and thus understanding the mechanisms underlying fetal survival have been the focus of intense research. In adults, survivin and Stat-3 proteins are involved in tolerance as well as the induction of apoptosis. However, the role of these molecules in pregnancy and development has not been addressed. We have evaluated the expression of survivin and Stat-3 in allogeneic mouse models of low abortions (CBA/J x Balb/c), abortion prone (CBA/J x DBA/2J) and stress-triggered abortions from DBA/2J-mated CBA/J mice. We show that survivin is over-expressed in abortion-prone mating on gestation day 7.5. This effect was also found in stress-exposed mice, whereas expression was low in normal pregnancy mice. The phosphorylated Stat-3 (p-Stat-3) was down regulated in high abortion mating compared with low abortion mating, CBA/J x Balb/c. The level of apoptosis was similar in the three groups studied. Our results suggest that high expression of survivin and low expression of p-Stat-3 are involved in pregnancy loss in mice.
AB - The materno-fetal interface has for long been considered as an immune privileged biological site and thus understanding the mechanisms underlying fetal survival have been the focus of intense research. In adults, survivin and Stat-3 proteins are involved in tolerance as well as the induction of apoptosis. However, the role of these molecules in pregnancy and development has not been addressed. We have evaluated the expression of survivin and Stat-3 in allogeneic mouse models of low abortions (CBA/J x Balb/c), abortion prone (CBA/J x DBA/2J) and stress-triggered abortions from DBA/2J-mated CBA/J mice. We show that survivin is over-expressed in abortion-prone mating on gestation day 7.5. This effect was also found in stress-exposed mice, whereas expression was low in normal pregnancy mice. The phosphorylated Stat-3 (p-Stat-3) was down regulated in high abortion mating compared with low abortion mating, CBA/J x Balb/c. The level of apoptosis was similar in the three groups studied. Our results suggest that high expression of survivin and low expression of p-Stat-3 are involved in pregnancy loss in mice.
KW - Abortion, Spontaneous/metabolism
KW - Animals
KW - Apoptosis
KW - Blotting, Western
KW - Decidua/chemistry
KW - Down-Regulation
KW - Embryo Implantation
KW - Female
KW - Humans
KW - Immunohistochemistry
KW - Inhibitor of Apoptosis Proteins
KW - Maternal-Fetal Exchange
KW - Mice
KW - Mice, Inbred Strains
KW - Microtubule-Associated Proteins/analysis
KW - Placenta/chemistry
KW - Pregnancy
KW - Repressor Proteins
KW - STAT3 Transcription Factor/analysis
KW - Survivin
KW - Up-Regulation
U2 - 10.1016/j.placenta.2006.09.010
DO - 10.1016/j.placenta.2006.09.010
M3 - SCORING: Journal article
C2 - 17113145
VL - 28
SP - 650
EP - 657
JO - PLACENTA
JF - PLACENTA
SN - 0143-4004
IS - 7
ER -
