Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.
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Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes. / Haberlandt, Christian; Derouiche, Amin; Wyczynski, Alexandra; Haseleu, Julia; Pohle, Jörg; Karram, Khalad; Trotter, Jacqueline; Seifert, Gerald; Frotscher, Michael; Steinhäuser, Christian; Jabs, Ronald.
in: PLOS ONE, Jahrgang 6, Nr. 3, 3, 2011, S. 17575.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.
AU - Haberlandt, Christian
AU - Derouiche, Amin
AU - Wyczynski, Alexandra
AU - Haseleu, Julia
AU - Pohle, Jörg
AU - Karram, Khalad
AU - Trotter, Jacqueline
AU - Seifert, Gerald
AU - Frotscher, Michael
AU - Steinhäuser, Christian
AU - Jabs, Ronald
PY - 2011
Y1 - 2011
N2 - NG2 cells, the fourth type of glia in the mammalian CNS, receive synaptic input from neurons. The function of this innervation is unknown yet. Postsynaptic changes in intracellular Ca(2+)-concentration ([Ca(2+)](i)) might be a possible consequence. We employed transgenic mice with fluorescently labeled NG2 cells to address this issue. To identify Ca(2+)-signaling pathways we combined patch-clamp recordings, Ca(2+)-imaging, mRNA-transcript analysis and focal pressure-application of various substances to identified NG2-cells in acute hippocampal slices. We show that activation of voltage-gated Ca(2+)-channels, Ca(2+)-permeable AMPA-receptors, and group I metabotropic glutamate-receptors provoke [Ca(2+)](i)-elevations in NG2 cells. The Ca(2+)-influx is amplified by Ca(2+)-induced Ca(2+)-release. Minimal electrical stimulation of presynaptic neurons caused postsynaptic currents but no somatic [Ca(2+)](i) elevations, suggesting that [Ca(2+)](i) elevations in NG2 cells might be restricted to their processes. Local Ca(2+)-signaling might provoke transmitter release or changes in cell motility. To identify structural prerequisites for such a scenario, we used electron microscopy, immunostaining, mRNA-transcript analysis, and time lapse imaging. We found that NG2 cells form symmetric and asymmetric synapses with presynaptic neurons and show immunoreactivity for vesicular glutamate transporter 1. The processes are actin-based, contain ezrin but not glial filaments, microtubules or endoplasmic reticulum. Furthermore, we demonstrate that NG2 cell processes in situ are highly motile. Our findings demonstrate that gray matter NG2 cells are endowed with the cellular machinery for two-way communication with neighboring cells.
AB - NG2 cells, the fourth type of glia in the mammalian CNS, receive synaptic input from neurons. The function of this innervation is unknown yet. Postsynaptic changes in intracellular Ca(2+)-concentration ([Ca(2+)](i)) might be a possible consequence. We employed transgenic mice with fluorescently labeled NG2 cells to address this issue. To identify Ca(2+)-signaling pathways we combined patch-clamp recordings, Ca(2+)-imaging, mRNA-transcript analysis and focal pressure-application of various substances to identified NG2-cells in acute hippocampal slices. We show that activation of voltage-gated Ca(2+)-channels, Ca(2+)-permeable AMPA-receptors, and group I metabotropic glutamate-receptors provoke [Ca(2+)](i)-elevations in NG2 cells. The Ca(2+)-influx is amplified by Ca(2+)-induced Ca(2+)-release. Minimal electrical stimulation of presynaptic neurons caused postsynaptic currents but no somatic [Ca(2+)](i) elevations, suggesting that [Ca(2+)](i) elevations in NG2 cells might be restricted to their processes. Local Ca(2+)-signaling might provoke transmitter release or changes in cell motility. To identify structural prerequisites for such a scenario, we used electron microscopy, immunostaining, mRNA-transcript analysis, and time lapse imaging. We found that NG2 cells form symmetric and asymmetric synapses with presynaptic neurons and show immunoreactivity for vesicular glutamate transporter 1. The processes are actin-based, contain ezrin but not glial filaments, microtubules or endoplasmic reticulum. Furthermore, we demonstrate that NG2 cell processes in situ are highly motile. Our findings demonstrate that gray matter NG2 cells are endowed with the cellular machinery for two-way communication with neighboring cells.
KW - Animals
KW - Humans
KW - Cells, Cultured
KW - Mice
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Microscopy, Fluorescence
KW - Mice, Transgenic
KW - Microscopy, Electron
KW - Electrophysiology
KW - Calcium/metabolism
KW - Signal Transduction/physiology
KW - Neuroglia/metabolism/ultrastructure
KW - Vesicular Glutamate Transport Protein 1/metabolism
KW - Animals
KW - Humans
KW - Cells, Cultured
KW - Mice
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Microscopy, Fluorescence
KW - Mice, Transgenic
KW - Microscopy, Electron
KW - Electrophysiology
KW - Calcium/metabolism
KW - Signal Transduction/physiology
KW - Neuroglia/metabolism/ultrastructure
KW - Vesicular Glutamate Transport Protein 1/metabolism
U2 - 10.1371/journal.pone.0017575
DO - 10.1371/journal.pone.0017575
M3 - SCORING: Journal article
VL - 6
SP - 17575
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 3
M1 - 3
ER -
