Granule cell dispersion in temporal lobe epilepsy is associated with changes in dendritic orientation and spine distribution.
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Granule cell dispersion in temporal lobe epilepsy is associated with changes in dendritic orientation and spine distribution. / Freiman, Thomas M; Eismann-Schweimler, Jessica; Frotscher, Michael.
in: EXP NEUROL, Jahrgang 229, Nr. 2, 2, 2011, S. 332-338.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Granule cell dispersion in temporal lobe epilepsy is associated with changes in dendritic orientation and spine distribution.
AU - Freiman, Thomas M
AU - Eismann-Schweimler, Jessica
AU - Frotscher, Michael
PY - 2011
Y1 - 2011
N2 - Granule cell dispersion is a characteristic feature of Ammon's horn sclerosis in temporal lobe epilepsy. It was recently shown that granule cell dispersion is associated with decreased expression of the extracellular matrix protein Reelin. Reelin controls neuronal lamination and the differentiation of dendrites and spines. Here, we studied dendritic orientation and the distribution of dendritic spines on granule cells in surgical specimens of patients suffering from temporal lobe epilepsy. In this material, we compared granule cells in dentate areas showing granule cell dispersion with granule cells in areas exhibiting a normal, densely packed granule cell layer. Granule cells (GC) were Golgi-stained and analyzed using a computer-based camera lucida system and were categorized as being located proximal or distal to the hilus (GCprox, GCdist). We found that GCprox in a densely packed granule cell layer exhibited a mainly vertically oriented dendritic arbor with a small bifurcation angle (17°) between branching dendrites. In contrast, GCdist in a densely packed granular layer showed a wider bifurcation angle (35°), suggesting a widening of the dendritic field during the migratory process to superficial positions. Granule cells in the dispersed granule cell layer showed an even wider bifurcation angle of their apical dendrites (GCprox: 40°; GCdist: 58°) and also exhibited recurrent basal dendrites. Spine density on dendrites of GCprox in dispersed areas was increased compared to GCprox in the normal, compact granule cell layer. In contrast, dendrites of GCdist extending into the molecular layer showed a reduced spine density in dispersed areas. The results are discussed in view of other findings on neuronal reorganization in the epileptic dentate gyrus.
AB - Granule cell dispersion is a characteristic feature of Ammon's horn sclerosis in temporal lobe epilepsy. It was recently shown that granule cell dispersion is associated with decreased expression of the extracellular matrix protein Reelin. Reelin controls neuronal lamination and the differentiation of dendrites and spines. Here, we studied dendritic orientation and the distribution of dendritic spines on granule cells in surgical specimens of patients suffering from temporal lobe epilepsy. In this material, we compared granule cells in dentate areas showing granule cell dispersion with granule cells in areas exhibiting a normal, densely packed granule cell layer. Granule cells (GC) were Golgi-stained and analyzed using a computer-based camera lucida system and were categorized as being located proximal or distal to the hilus (GCprox, GCdist). We found that GCprox in a densely packed granule cell layer exhibited a mainly vertically oriented dendritic arbor with a small bifurcation angle (17°) between branching dendrites. In contrast, GCdist in a densely packed granular layer showed a wider bifurcation angle (35°), suggesting a widening of the dendritic field during the migratory process to superficial positions. Granule cells in the dispersed granule cell layer showed an even wider bifurcation angle of their apical dendrites (GCprox: 40°; GCdist: 58°) and also exhibited recurrent basal dendrites. Spine density on dendrites of GCprox in dispersed areas was increased compared to GCprox in the normal, compact granule cell layer. In contrast, dendrites of GCdist extending into the molecular layer showed a reduced spine density in dispersed areas. The results are discussed in view of other findings on neuronal reorganization in the epileptic dentate gyrus.
KW - Adult
KW - Humans
KW - Male
KW - Female
KW - Middle Aged
KW - Hippocampus/pathology
KW - Dendrites/pathology
KW - Dendritic Spines/pathology
KW - Epilepsy, Temporal Lobe/pathology
KW - Neurons/pathology
KW - Silver Staining
KW - Adult
KW - Humans
KW - Male
KW - Female
KW - Middle Aged
KW - Hippocampus/pathology
KW - Dendrites/pathology
KW - Dendritic Spines/pathology
KW - Epilepsy, Temporal Lobe/pathology
KW - Neurons/pathology
KW - Silver Staining
M3 - SCORING: Journal article
VL - 229
SP - 332
EP - 338
JO - EXP NEUROL
JF - EXP NEUROL
SN - 0014-4886
IS - 2
M1 - 2
ER -
