Developmental refinement of vesicle cycling at Schaffer collateral synapses
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Developmental refinement of vesicle cycling at Schaffer collateral synapses. / Rose, Tobias; Schoenenberger, Philipp; Jezek, Karel; Oertner, Thomas G.
in: NEURON, Jahrgang 77, Nr. 6, 20.03.2013, S. 1109-21.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Developmental refinement of vesicle cycling at Schaffer collateral synapses
AU - Rose, Tobias
AU - Schoenenberger, Philipp
AU - Jezek, Karel
AU - Oertner, Thomas G
N1 - Copyright © 2013 Elsevier Inc. All rights reserved.
PY - 2013/3/20
Y1 - 2013/3/20
N2 - At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called "resting pool." Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.
AB - At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called "resting pool." Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.
KW - Animals
KW - Animals, Newborn
KW - CA1 Region, Hippocampal
KW - Endocytosis
KW - Organ Culture Techniques
KW - Rats
KW - Rats, Wistar
KW - Synapses
KW - Synaptic Vesicles
U2 - 10.1016/j.neuron.2013.01.021
DO - 10.1016/j.neuron.2013.01.021
M3 - SCORING: Journal article
C2 - 23522046
VL - 77
SP - 1109
EP - 1121
JO - NEURON
JF - NEURON
SN - 0896-6273
IS - 6
ER -