Detection of invasive Trichosporon asahii in patient blood by a fungal PCR array
Standard
Detection of invasive Trichosporon asahii in patient blood by a fungal PCR array. / Weber, Jasmin K; Scharf, Sebastian; Walther, Grit; Flüh, Greta; MacKenzie, Colin R; Kondakci, Mustafa; Henrich, Birgit; Kohns Vasconcelos, Malte.
in: Access microbiology, Jahrgang 3, Nr. 12, 000285, 2021.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › Case Report › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Detection of invasive Trichosporon asahii in patient blood by a fungal PCR array
AU - Weber, Jasmin K
AU - Scharf, Sebastian
AU - Walther, Grit
AU - Flüh, Greta
AU - MacKenzie, Colin R
AU - Kondakci, Mustafa
AU - Henrich, Birgit
AU - Kohns Vasconcelos, Malte
N1 - © 2021 The Authors.
PY - 2021
Y1 - 2021
N2 - Rare invasive fungal infections are increasingly emerging in hosts with predisposing factors such as immunodeficiency. Their timely diagnosis remains difficult, as their clinical picture may initially mimic infections with more common fungal species and species identification may be difficult with routine methods or may require time-consuming subcultures. This often results in ineffective drug administration and fatal outcomes. We report on a patient in their early twenties with mixed cellularity classical Hodgkin lymphoma with a disseminated Trichosporon asahii (T. asahii) infection. Even though pathogen detection and identification was possible via the standard procedure consisting of culture followed by matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry, the patient passed away in the course of multi organ failure. Herein, we report on a retrospectively applied experimental diagnostic fungal PCR-analysis used on an EDTA blood sample and consisting of two pan-fungal reactions and seven branch-specific reactions. Regarding invasive T. asahii infection, this PCR array could considerably shorten time to diagnosis and switch to a targeted therapy with triazoles.
AB - Rare invasive fungal infections are increasingly emerging in hosts with predisposing factors such as immunodeficiency. Their timely diagnosis remains difficult, as their clinical picture may initially mimic infections with more common fungal species and species identification may be difficult with routine methods or may require time-consuming subcultures. This often results in ineffective drug administration and fatal outcomes. We report on a patient in their early twenties with mixed cellularity classical Hodgkin lymphoma with a disseminated Trichosporon asahii (T. asahii) infection. Even though pathogen detection and identification was possible via the standard procedure consisting of culture followed by matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry, the patient passed away in the course of multi organ failure. Herein, we report on a retrospectively applied experimental diagnostic fungal PCR-analysis used on an EDTA blood sample and consisting of two pan-fungal reactions and seven branch-specific reactions. Regarding invasive T. asahii infection, this PCR array could considerably shorten time to diagnosis and switch to a targeted therapy with triazoles.
U2 - 10.1099/acmi.0.000285
DO - 10.1099/acmi.0.000285
M3 - Case report
C2 - 35024550
VL - 3
IS - 12
M1 - 000285
ER -
