Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents

Standard

Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents. / Romm, H; Ainsbury, E; Barnard, S; Barrios, L; Barquinero, J F; Beinke, C; Deperas, M; Gregoire, E; Koivistoinen, A; Lindholm, C; Moquet, J; Oestreicher, U; Puig, R; Rothkamm, K; Sommer, S; Thierens, H; Vandersickel, V; Vral, A; Wojcik, A.

in: MUTAT RES-FUND MOL M, Jahrgang 756, Nr. 1-2, 30.08.2013, S. 174-83.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

Harvard

Romm, H, Ainsbury, E, Barnard, S, Barrios, L, Barquinero, JF, Beinke, C, Deperas, M, Gregoire, E, Koivistoinen, A, Lindholm, C, Moquet, J, Oestreicher, U, Puig, R, Rothkamm, K, Sommer, S, Thierens, H, Vandersickel, V, Vral, A & Wojcik, A 2013, 'Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents', MUTAT RES-FUND MOL M, Jg. 756, Nr. 1-2, S. 174-83. https://doi.org/10.1016/j.mrgentox.2013.05.013

APA

Romm, H., Ainsbury, E., Barnard, S., Barrios, L., Barquinero, J. F., Beinke, C., Deperas, M., Gregoire, E., Koivistoinen, A., Lindholm, C., Moquet, J., Oestreicher, U., Puig, R., Rothkamm, K., Sommer, S., Thierens, H., Vandersickel, V., Vral, A., & Wojcik, A. (2013). Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents. MUTAT RES-FUND MOL M, 756(1-2), 174-83. https://doi.org/10.1016/j.mrgentox.2013.05.013

Vancouver

Romm H, Ainsbury E, Barnard S, Barrios L, Barquinero JF, Beinke C et al. Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents. MUTAT RES-FUND MOL M. 2013 Aug 30;756(1-2):174-83. https://doi.org/10.1016/j.mrgentox.2013.05.013

Bibtex

@article{a8a1043536ec4ea8a0902e33cd61e282,
title = "Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents",
abstract = "Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage in order to rapidly identify individuals who require clinical treatment. The manual dicentric assay is a highly suitable technique, but it is also very time consuming and requires well trained scorers. In the framework of the MULTIBIODOSE EU FP7 project, semi-automated dicentric scoring has been established in six European biodosimetry laboratories. Whole blood was irradiated with a Co-60 gamma source resulting in 8 different doses between 0 and 4.5Gy and then shipped to the six participating laboratories. To investigate two different scoring strategies, cell cultures were set up with short term (2-3h) or long term (24h) colcemid treatment. Three classifiers for automatic dicentric detection were applied, two of which were developed specifically for these two different culture techniques. The automation procedure included metaphase finding, capture of cells at high resolution and detection of dicentric candidates. The automatically detected dicentric candidates were then evaluated by a trained human scorer, which led to the term 'semi-automated' being applied to the analysis. The six participating laboratories established at least one semi-automated calibration curve each, using the appropriate classifier for their colcemid treatment time. There was no significant difference between the calibration curves established, regardless of the classifier used. The ratio of false positive to true positive dicentric candidates was dose dependent. The total staff effort required for analysing 150 metaphases using the semi-automated approach was 2 min as opposed to 60 min for manual scoring of 50 metaphases. Semi-automated dicentric scoring is a useful tool in a large scale radiation accident as it enables high throughput screening of samples for fast triage of potentially exposed individuals. Furthermore, the results from the participating laboratories were comparable which supports networking between laboratories for this assay.",
keywords = "Automation, Chromosome Aberrations/radiation effects, Chromosomes, Human/radiation effects, Cobalt Radioisotopes, Dose-Response Relationship, Radiation, Europe, Gamma Rays/adverse effects, Humans, Laboratories/standards, Lymphocytes/radiation effects, Radiation Monitoring/methods, Radioactive Hazard Release/prevention & control",
author = "H Romm and E Ainsbury and S Barnard and L Barrios and Barquinero, {J F} and C Beinke and M Deperas and E Gregoire and A Koivistoinen and C Lindholm and J Moquet and U Oestreicher and R Puig and K Rothkamm and S Sommer and H Thierens and V Vandersickel and A Vral and A Wojcik",
note = "Copyright {\textcopyright} 2013 Elsevier B.V. All rights reserved.",
year = "2013",
month = aug,
day = "30",
doi = "10.1016/j.mrgentox.2013.05.013",
language = "English",
volume = "756",
pages = "174--83",
number = "1-2",

}

RIS

TY - JOUR

T1 - Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents

AU - Romm, H

AU - Ainsbury, E

AU - Barnard, S

AU - Barrios, L

AU - Barquinero, J F

AU - Beinke, C

AU - Deperas, M

AU - Gregoire, E

AU - Koivistoinen, A

AU - Lindholm, C

AU - Moquet, J

AU - Oestreicher, U

AU - Puig, R

AU - Rothkamm, K

AU - Sommer, S

AU - Thierens, H

AU - Vandersickel, V

AU - Vral, A

AU - Wojcik, A

N1 - Copyright © 2013 Elsevier B.V. All rights reserved.

PY - 2013/8/30

Y1 - 2013/8/30

N2 - Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage in order to rapidly identify individuals who require clinical treatment. The manual dicentric assay is a highly suitable technique, but it is also very time consuming and requires well trained scorers. In the framework of the MULTIBIODOSE EU FP7 project, semi-automated dicentric scoring has been established in six European biodosimetry laboratories. Whole blood was irradiated with a Co-60 gamma source resulting in 8 different doses between 0 and 4.5Gy and then shipped to the six participating laboratories. To investigate two different scoring strategies, cell cultures were set up with short term (2-3h) or long term (24h) colcemid treatment. Three classifiers for automatic dicentric detection were applied, two of which were developed specifically for these two different culture techniques. The automation procedure included metaphase finding, capture of cells at high resolution and detection of dicentric candidates. The automatically detected dicentric candidates were then evaluated by a trained human scorer, which led to the term 'semi-automated' being applied to the analysis. The six participating laboratories established at least one semi-automated calibration curve each, using the appropriate classifier for their colcemid treatment time. There was no significant difference between the calibration curves established, regardless of the classifier used. The ratio of false positive to true positive dicentric candidates was dose dependent. The total staff effort required for analysing 150 metaphases using the semi-automated approach was 2 min as opposed to 60 min for manual scoring of 50 metaphases. Semi-automated dicentric scoring is a useful tool in a large scale radiation accident as it enables high throughput screening of samples for fast triage of potentially exposed individuals. Furthermore, the results from the participating laboratories were comparable which supports networking between laboratories for this assay.

AB - Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage in order to rapidly identify individuals who require clinical treatment. The manual dicentric assay is a highly suitable technique, but it is also very time consuming and requires well trained scorers. In the framework of the MULTIBIODOSE EU FP7 project, semi-automated dicentric scoring has been established in six European biodosimetry laboratories. Whole blood was irradiated with a Co-60 gamma source resulting in 8 different doses between 0 and 4.5Gy and then shipped to the six participating laboratories. To investigate two different scoring strategies, cell cultures were set up with short term (2-3h) or long term (24h) colcemid treatment. Three classifiers for automatic dicentric detection were applied, two of which were developed specifically for these two different culture techniques. The automation procedure included metaphase finding, capture of cells at high resolution and detection of dicentric candidates. The automatically detected dicentric candidates were then evaluated by a trained human scorer, which led to the term 'semi-automated' being applied to the analysis. The six participating laboratories established at least one semi-automated calibration curve each, using the appropriate classifier for their colcemid treatment time. There was no significant difference between the calibration curves established, regardless of the classifier used. The ratio of false positive to true positive dicentric candidates was dose dependent. The total staff effort required for analysing 150 metaphases using the semi-automated approach was 2 min as opposed to 60 min for manual scoring of 50 metaphases. Semi-automated dicentric scoring is a useful tool in a large scale radiation accident as it enables high throughput screening of samples for fast triage of potentially exposed individuals. Furthermore, the results from the participating laboratories were comparable which supports networking between laboratories for this assay.

KW - Automation

KW - Chromosome Aberrations/radiation effects

KW - Chromosomes, Human/radiation effects

KW - Cobalt Radioisotopes

KW - Dose-Response Relationship, Radiation

KW - Europe

KW - Gamma Rays/adverse effects

KW - Humans

KW - Laboratories/standards

KW - Lymphocytes/radiation effects

KW - Radiation Monitoring/methods

KW - Radioactive Hazard Release/prevention & control

U2 - 10.1016/j.mrgentox.2013.05.013

DO - 10.1016/j.mrgentox.2013.05.013

M3 - SCORING: Journal article

C2 - 23707243

VL - 756

SP - 174

EP - 183

IS - 1-2

ER -